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000275851 0247_ $$2ISSN$$a0177-3593
000275851 0247_ $$2ISSN$$a0372-9672
000275851 0247_ $$2ISSN$$a1431-6730
000275851 0247_ $$2ISSN$$a1437-4315
000275851 037__ $$aDZNE-2025-00086
000275851 041__ $$aEnglish
000275851 1001_ $$00000-0003-0925-0308$$aVogt, Arend$$b0
000275851 245__ $$aSimultaneous spectral illumination of microplates for high-throughput optogenetics and photobiology.
000275851 260__ $$c2024
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000275851 520__ $$aThe biophysical characterization and engineering of optogenetic tools and photobiological systems has been hampered by the lack of efficient methods for spectral illumination of microplates for high-throughput analysis of action spectra. Current methods to determine action spectra only allow the sequential spectral illumination of individual wells. Here we present the open-source RainbowCap-system, which combines LEDs and optical filters in a standard 96-well microplate format for simultaneous and spectrally defined illumination. The RainbowCap provides equal photon flux for each wavelength, with the output of the LEDs narrowed by optical bandpass filters. We validated the RainbowCap for photoactivatable G protein-coupled receptors (opto-GPCRs) and enzymes for the control of intracellular downstream signaling. The simultaneous, spectrally defined illumination provides minimal interruption during time-series measurements, while resolving 10 nm differences in the action spectra of optogenetic proteins under identical experimental conditions. The RainbowCap is also suitable for studying the spectral dependence of light-regulated gene expression in bacteria, which requires illumination over several hours. In summary, the RainbowCap provides high-throughput spectral illumination of microplates, while its modular, customizable design allows easy adaptation to a wide range of optogenetic and photobiological applications.
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000275851 650_7 $$2Other$$aGPCR
000275851 650_7 $$2Other$$aaction spectra
000275851 650_7 $$2Other$$acyclic mononucleotides
000275851 650_7 $$2Other$$aphotoactivated nucleotidyl cyclases
000275851 650_7 $$2Other$$arhodopsin
000275851 650_7 $$2Other$$asignal transduction
000275851 650_7 $$2NLM Chemicals$$aReceptors, G-Protein-Coupled
000275851 650_2 $$2MeSH$$aOptogenetics: methods
000275851 650_2 $$2MeSH$$aOptogenetics: instrumentation
000275851 650_2 $$2MeSH$$aPhotobiology
000275851 650_2 $$2MeSH$$aReceptors, G-Protein-Coupled: metabolism
000275851 650_2 $$2MeSH$$aReceptors, G-Protein-Coupled: genetics
000275851 650_2 $$2MeSH$$aHigh-Throughput Screening Assays
000275851 650_2 $$2MeSH$$aLight
000275851 650_2 $$2MeSH$$aHumans
000275851 7001_ $$00000-0002-1810-6036$$aPaulat, Raik$$b1
000275851 7001_ $$00000-0001-8775-024X$$aParthier, Daniel$$b2
000275851 7001_ $$aJust, Verena$$b3
000275851 7001_ $$00000-0001-5079-6893$$aSzczepek, Michal$$b4
000275851 7001_ $$00000-0001-5028-2075$$aScheerer, Patrick$$b5
000275851 7001_ $$aXu, Qianzhao$$b6
000275851 7001_ $$00000-0002-7382-2772$$aMöglich, Andreas$$b7
000275851 7001_ $$0P:(DE-2719)2810725$$aSchmitz, Dietmar$$b8
000275851 7001_ $$0P:(DE-2719)2810914$$aRost, Benjamin R$$b9
000275851 7001_ $$00000-0002-0965-7530$$aWenger, Nikolaus$$b10
000275851 773__ $$0PERI:(DE-600)1466062-3$$a10.1515/hsz-2023-0205$$gVol. 405, no. 11-12, p. 751 - 763$$n11-12$$p751 - 763$$t (0018-4888)$$v405$$x0018-4888$$y2024
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