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000275866 1001_ $$00000-0002-3957-3848$$aMorton, Lorena$$b0
000275866 245__ $$aPericytes and Extracellular Vesicle Interactions in Neurovascular Adaptation to Chronic Arterial Hypertension.
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000275866 520__ $$aChronic arterial hypertension restructures the vascular architecture of the brain, leading to a series of pathological responses that culminate in cerebral small-vessel disease. Pericytes respond dynamically to vascular challenges; however, how they manifest under the continuous strain of hypertension has not been elucidated.In this study, we characterized pericyte behavior alongside hypertensive states in the spontaneously hypertensive stroke-prone rat model, focusing on their phenotypic and metabolic transformation. Flow cytometry was used to characterize pericytes by their expression of platelet-derived growth factor receptor β, neuroglial antigen 2, cluster of differentiation 13-alanyl aminopeptidase, and antigen Kiel 67. Microvessels were isolated for gene expression profiling and in vitro pericyte expansion. Immunofluorescence validated the cell culture model. Plasma-derived extracellular vesicles from hypertensive rodents were applied as a treatment to assess their effects on pericyte function and detailed metabolic assessments on enriched pericytes measured oxidative phosphorylation and glycolysis. Our results reveal a shift in platelet-derived growth factor receptor β+ pericytes toward increased neuroglial antigen 2 and cluster of differentiation 13-alanyl aminopeptidase coexpression, indicative of their critical role in vascular stabilization and inflammatory responses within the hypertensive milieu. Significant alterations were found within key pathways including angiogenesis, blood-brain barrier integrity, hypoxia, and inflammation. Circulating extracellular vesicles from hypertensive rodents distinctly influenced pericyte mitochondrial function, evidencing their dual role as carriers of disease pathology and potential therapeutic agents. Furthermore, a shift toward glycolytic metabolism in hypertensive pericytes was confirmed, coupled with ATP production dysregulation.Our findings demonstrate that cerebral pericytes undergo phenotypic and metabolic reprogramming in response to hypertension, with hypertensive-derived plasma-derived extracellular vesicles impairing their mitochondrial function. Importantly, plasma-derived extracellular vesicles from normotensive controls restore this function, suggesting their potential as both therapeutic agents and precision biomarkers for hypertensive vascular complications. Further investigation into plasma-derived extracellular vesicle cargo is essential to further explore their therapeutic potential in vascular health.
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000275866 650_7 $$2Other$$ablood–brain barrier
000275866 650_7 $$2Other$$aextracellular vesicles
000275866 650_7 $$2Other$$ahypertension
000275866 650_7 $$2Other$$amitochondrial membrane potential
000275866 650_7 $$2Other$$apericytes
000275866 650_7 $$2Other$$aspontaneously hypertensive stroke‐prone rat (SHRSP)
000275866 650_7 $$2Other$$avascular remodeling
000275866 650_2 $$2MeSH$$aPericytes: metabolism
000275866 650_2 $$2MeSH$$aPericytes: pathology
000275866 650_2 $$2MeSH$$aAnimals
000275866 650_2 $$2MeSH$$aExtracellular Vesicles: metabolism
000275866 650_2 $$2MeSH$$aHypertension: physiopathology
000275866 650_2 $$2MeSH$$aHypertension: metabolism
000275866 650_2 $$2MeSH$$aRats, Inbred SHR
000275866 650_2 $$2MeSH$$aMale
000275866 650_2 $$2MeSH$$aRats
000275866 650_2 $$2MeSH$$aDisease Models, Animal
000275866 650_2 $$2MeSH$$aChronic Disease
000275866 650_2 $$2MeSH$$aCells, Cultured
000275866 650_2 $$2MeSH$$aAdaptation, Physiological
000275866 650_2 $$2MeSH$$aBlood-Brain Barrier: metabolism
000275866 650_2 $$2MeSH$$aBlood-Brain Barrier: pathology
000275866 7001_ $$00000-0002-2993-7679$$aGarza, Alejandra P$$b1
000275866 7001_ $$0P:(DE-2719)9000903$$aDebska-Vielhaber, Grazyna$$b2
000275866 7001_ $$00009-0003-2930-2635$$aVillafuerte, Luis E$$b3
000275866 7001_ $$0P:(DE-2719)2813348$$aHenneicke, Solveig$$b4
000275866 7001_ $$0P:(DE-2719)9000797$$aUlbrich, Philipp$$b5$$udzne
000275866 7001_ $$00000-0003-2571-3501$$aMeuth, Sven G$$b6
000275866 7001_ $$0P:(DE-2719)2812631$$aSchreiber, Stefanie$$b7
000275866 7001_ $$00000-0002-9900-8605$$aDunay, Ildiko R$$b8
000275866 773__ $$0PERI:(DE-600)2653953-6$$a10.1161/JAHA.124.038457$$gVol. 14, no. 1, p. e038457$$n1$$pe038457$$tJournal of the American Heart Association$$v14$$x2047-9980$$y2025
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