Home > Publications Database > MRI-based morphometric structural changes correlate with histopathology in experimental autoimmune encephalomyelitis. > print

001     275886
005     20250120165733.0
024 7 _ |a 10.1016/j.jns.2024.123358
|2 doi
024 7 _ |a pmid:39729930
|2 pmid
024 7 _ |a 0022-510X
|2 ISSN
024 7 _ |a 1878-5883
|2 ISSN
037 _ _ |a DZNE-2025-00121
041 _ _ |a English
082 _ _ |a 610
100 1 _ |a Lowinski, Anna
|b 0
245 _ _ |a MRI-based morphometric structural changes correlate with histopathology in experimental autoimmune encephalomyelitis.
260 _ _ |a Amsterdam [u.a.]
|c 2025
|b Elsevier Science
336 7 _ |a article
|2 DRIVER
336 7 _ |a Output Types/Journal article
|2 DataCite
336 7 _ |a Journal Article
|b journal
|m journal
|0 PUB:(DE-HGF)16
|s 1737366129_5001
|2 PUB:(DE-HGF)
336 7 _ |a ARTICLE
|2 BibTeX
336 7 _ |a JOURNAL_ARTICLE
|2 ORCID
336 7 _ |a Journal Article
|0 0
|2 EndNote
520 _ _ |a Magnetic resonance imaging (MRI) and neurohistopathology are important correlates for evaluation of disease progression in multiple sclerosis (MS). Here we used experimental autoimmune encephalomyelitis (EAE) as an animal model of MS to determine the correlation between clinical EAE severity, MRI and histopathological parameters.N = 11 female C57BL/6J mice were immunized with human myelin oligodendrocyte glycoprotein 1-125, while N = 9 remained non-immunized. Mice were scanned longitudinally over a period of 13 weeks using a 11.7 Tesla (T) Bruker BioSpec® preclinical MRI instrument, and regional volume changes of the lumbar spinal cord were analyzed using Voxel-Guided Morphometry (VGM). Following the final in vivo T1-weighted MRI scan, the lumbar spinal cord of each mouse was subjected to an ex vivo MRI scan using T1-, T2*- and diffusion tensor imaging (DTI)-weighted sequences. Tissue sections were then stained for immune cell infiltration, demyelination, astrogliosis, and axonal damage using hematoxylin-eosin staining and immunohistochemistry.While in vivo MRI VGM detected an overall increase in volume over time, no differences were observed between EAE animals and controls. Ex vivo MRI showed a generalized atrophy of the spinal cord, which was pronounced in the anterolateral tract. The most striking correlation was observed between EAE score, white matter atrophy in ex vivo T1-weighted scans and histological parameters.The data demonstrate that ex vivo MRI is a valuable tool to assess white matter atrophy in EAE, which was shown to be directly linked to the severity of EAE and spinal cord histopathology.
536 _ _ |a 899 - ohne Topic (POF4-899)
|0 G:(DE-HGF)POF4-899
|c POF4-899
|f POF IV
|x 0
588 _ _ |a Dataset connected to CrossRef, PubMed, , Journals: pub.dzne.de
650 _ 7 |a Experimental autoimmune encephalomyelitis
|2 Other
650 _ 7 |a Magnetic resonance imaging
|2 Other
650 _ 7 |a Multiple sclerosis
|2 Other
650 _ 7 |a T1
|2 Other
650 _ 7 |a T2
|2 Other
650 _ 7 |a T2*
|2 Other
650 _ 7 |a Voxel-guided morphometry
|2 Other
650 _ 7 |a Myelin-Oligodendrocyte Glycoprotein
|2 NLM Chemicals
650 _ 2 |a Encephalomyelitis, Autoimmune, Experimental: pathology
|2 MeSH
650 _ 2 |a Encephalomyelitis, Autoimmune, Experimental: diagnostic imaging
|2 MeSH
650 _ 2 |a Animals
|2 MeSH
650 _ 2 |a Female
|2 MeSH
650 _ 2 |a Mice, Inbred C57BL
|2 MeSH
650 _ 2 |a Magnetic Resonance Imaging: methods
|2 MeSH
650 _ 2 |a Spinal Cord: pathology
|2 MeSH
650 _ 2 |a Spinal Cord: diagnostic imaging
|2 MeSH
650 _ 2 |a Mice
|2 MeSH
650 _ 2 |a Disease Models, Animal
|2 MeSH
650 _ 2 |a Myelin-Oligodendrocyte Glycoprotein: immunology
|2 MeSH
650 _ 2 |a Diffusion Tensor Imaging: methods
|2 MeSH
700 1 _ |a Dabringhaus, Andreas
|b 1
700 1 _ |a Kraemer, Matthias
|b 2
700 1 _ |a Doshi, Hardik
|0 P:(DE-2719)2814350
|b 3
|u dzne
700 1 _ |a Weier, Alicia
|b 4
700 1 _ |a Hintze, Maik
|b 5
700 1 _ |a Chunder, Rittika
|b 6
700 1 _ |a Kuerten, Stefanie
|b 7
773 _ _ |a 10.1016/j.jns.2024.123358
|g Vol. 468, p. 123358 -
|0 PERI:(DE-600)1500645-1
|p 123358
|t Journal of the neurological sciences
|v 468
|y 2025
|x 0022-510X
856 4 _ |y OpenAccess
|u https://pub.dzne.de/record/275886/files/DZNE-2025-00121.pdf
856 4 _ |y OpenAccess
|x pdfa
|u https://pub.dzne.de/record/275886/files/DZNE-2025-00121.pdf?subformat=pdfa
909 C O |o oai:pub.dzne.de:275886
|p openaire
|p open_access
|p VDB
|p driver
|p dnbdelivery
910 1 _ |a Deutsches Zentrum für Neurodegenerative Erkrankungen
|0 I:(DE-588)1065079516
|k DZNE
|b 3
|6 P:(DE-2719)2814350
913 1 _ |a DE-HGF
|b Programmungebundene Forschung
|l ohne Programm
|1 G:(DE-HGF)POF4-890
|0 G:(DE-HGF)POF4-899
|3 G:(DE-HGF)POF4
|2 G:(DE-HGF)POF4-800
|4 G:(DE-HGF)POF
|v ohne Topic
|x 0
914 1 _ |y 2025
915 _ _ |a DBCoverage
|0 StatID:(DE-HGF)0200
|2 StatID
|b SCOPUS
|d 2024-12-06
915 _ _ |a DBCoverage
|0 StatID:(DE-HGF)0160
|2 StatID
|b Essential Science Indicators
|d 2024-12-06
915 _ _ |a DBCoverage
|0 StatID:(DE-HGF)1050
|2 StatID
|b BIOSIS Previews
|d 2024-12-06
915 _ _ |a DBCoverage
|0 StatID:(DE-HGF)1190
|2 StatID
|b Biological Abstracts
|d 2024-12-06
915 _ _ |a DBCoverage
|0 StatID:(DE-HGF)0600
|2 StatID
|b Ebsco Academic Search
|d 2024-12-06
915 _ _ |a JCR
|0 StatID:(DE-HGF)0100
|2 StatID
|b J NEUROL SCI : 2022
|d 2024-12-06
915 _ _ |a DBCoverage
|0 StatID:(DE-HGF)1030
|2 StatID
|b Current Contents - Life Sciences
|d 2024-12-06
915 _ _ |a WoS
|0 StatID:(DE-HGF)0113
|2 StatID
|b Science Citation Index Expanded
|d 2024-12-06
915 _ _ |a DBCoverage
|0 StatID:(DE-HGF)0150
|2 StatID
|b Web of Science Core Collection
|d 2024-12-06
915 _ _ |a IF < 5
|0 StatID:(DE-HGF)9900
|2 StatID
|d 2024-12-06
915 _ _ |a OpenAccess
|0 StatID:(DE-HGF)0510
|2 StatID
915 _ _ |a Peer Review
|0 StatID:(DE-HGF)0030
|2 StatID
|b ASC
|d 2024-12-06
915 _ _ |a DBCoverage
|0 StatID:(DE-HGF)0300
|2 StatID
|b Medline
|d 2024-12-06
915 _ _ |a Creative Commons Attribution CC BY 4.0
|0 LIC:(DE-HGF)CCBY4
|2 HGFVOC
915 _ _ |a Nationallizenz
|0 StatID:(DE-HGF)0420
|2 StatID
|d 2024-12-06
|w ger
915 _ _ |a DBCoverage
|0 StatID:(DE-HGF)0199
|2 StatID
|b Clarivate Analytics Master Journal List
|d 2024-12-06
920 1 _ |0 I:(DE-2719)1040270
|k SAMRI
|l Small Animal MRI (CRFS-SAMRI)
|x 0
920 1 _ |0 I:(DE-2719)1040000
|k CRFS
|l Core Research Facilities & Services
|x 1
980 _ _ |a journal
980 _ _ |a VDB
980 _ _ |a UNRESTRICTED
980 _ _ |a I:(DE-2719)1040270
980 _ _ |a I:(DE-2719)1040000
980 1 _ |a FullTexts

LibraryCollectionCLSMajorCLSMinorLanguageAuthor
Marc 21