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@ARTICLE{Abaei:276086,
      author       = {Abaei, Alireza and Deelchand, Dinesh K and Kassubek, Jan
                      and Roselli, Francescois and Rasche, Volker},
      title        = {{S}ub-{M}icroliter 1{H} {M}agnetic {R}esonance
                      {S}pectroscopy for {I}n {V}ivo {H}igh-{S}patial {R}esolution
                      {M}etabolite {Q}uantification in the {M}ouse {B}rain.},
      journal      = {Journal of neurochemistry},
      volume       = {169},
      number       = {1},
      issn         = {0022-3042},
      address      = {Oxford},
      publisher    = {Wiley-Blackwell},
      reportid     = {DZNE-2025-00167},
      pages        = {e16303},
      year         = {2025},
      abstract     = {Proton magnetic resonance spectroscopy (MRS) offers a
                      non-invasive, repeatable, and reproducible method for in
                      vivo metabolite profiling of the brain and other tissues.
                      However, metabolite fingerprinting by MRS requires high
                      signal-to-noise ratios for accurate metabolite
                      quantification, which has traditionally been limited to
                      large volumes of interest, compromising spatial fidelity. In
                      this study, we introduce a new optimized pipeline that
                      combines LASER MRS acquisition at 11.7 T with a cryogenic
                      coil and advanced offline pre- and post-processing. This
                      approach achieves a signal-to-noise ratio sufficient to
                      reliably quantify 19 distinct metabolites in a volume as
                      small as 0.7 μL within the mouse brain. The resulting high
                      spatial resolution and spectral quality enable the
                      identification of distinct metabolite fingerprints in small,
                      specific regions, as demonstrated by characteristic
                      differences in N-acetylaspartate, glutamate, taurine, and
                      myo-inositol between the motor and somatosensory cortices.
                      We demonstrated a decline in taurine and glutamate in the
                      primary motor cortex between 5 and 11 months of age, against
                      the stability of other metabolites. Further exploitation to
                      cortical layer-specific metabolite fingerprinting of layer
                      I-III to layer VI-V in the primary motor cortex, with the
                      latter showing reduced taurine and phosphoethanolamine
                      levels, demonstrates the potential of this pipeline for
                      detailed in vivo metabolite fingerprinting of cortical areas
                      and subareas.},
      keywords     = {Animals / Mice / Proton Magnetic Resonance Spectroscopy:
                      methods / Mice, Inbred C57BL / Male / Brain: metabolism /
                      Brain: diagnostic imaging / Taurine: metabolism / Taurine:
                      analysis / Glutamic Acid: metabolism / Glutamic Acid:
                      analysis / Female / Inositol: metabolism / cortical areas
                      (Other) / high spatial resolution (Other) / magnetic
                      resonance spectroscopy (Other) / metabolite profile (Other)
                      / Taurine (NLM Chemicals) / Glutamic Acid (NLM Chemicals) /
                      Inositol (NLM Chemicals)},
      cin          = {Clinical Study Center (Ulm) / AG Roselli},
      ddc          = {610},
      cid          = {I:(DE-2719)5000077 / I:(DE-2719)1910001},
      pnm          = {353 - Clinical and Health Care Research (POF4-353) / 352 -
                      Disease Mechanisms (POF4-352)},
      pid          = {G:(DE-HGF)POF4-353 / G:(DE-HGF)POF4-352},
      typ          = {PUB:(DE-HGF)16},
      pmc          = {pmc:PMC11742661},
      pubmed       = {pmid:39825728},
      doi          = {10.1111/jnc.16303},
      url          = {https://pub.dzne.de/record/276086},
}