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@ARTICLE{Arshad:276154,
author = {Arshad, Hamza and Eid, Shehab and Mehra, Surabhi and
Williams, Declan and Kaczmarczyk, Lech and Stuart, Erica and
Jackson, Walker Scot and Schmitt-Ulms, Gerold and Watts,
Joel C},
title = {{T}he brain interactome of a permissive prion replication
substrate.},
journal = {Neurobiology of disease},
volume = {206},
issn = {0969-9961},
address = {[Amsterdam]},
publisher = {Elsevier},
reportid = {DZNE-2025-00226},
pages = {106802},
year = {2025},
abstract = {Bank voles are susceptible to prion strains from many
different species, yet the molecular mechanisms underlying
the ability of bank vole prion protein (BVPrP) to function
as a universal prion acceptor remain unclear. Potential
differences in molecular environments and protein
interaction networks on the cell surface of brain cells may
contribute to BVPrP's unusual behavior. To test this
hypothesis, we generated knock-in mice that express
physiological levels of BVPrP (M109 isoform) and employed
mass spectrometry to compare the interactomes of mouse (Mo)
PrP and BVPrP following mild in vivo crosslinking of brain
tissue. Substantial overlap was observed between the top
interactors for BVPrP and MoPrP, with established
PrP-interactors such as neural cell adhesion molecules,
subunits of Na+/K+-ATPases, and contactin-1 being equally
present in the two interactomes. We conclude that the
molecular environments of BVPrP and MoPrP in the brains of
mice are very similar. This suggests that the unorthodox
properties of BVPrP are unlikely to be mediated by
differential interactions with other proteins.},
keywords = {Bank voles (Other) / Knock-in mice (Other) / Mass
spectrometry (Other) / Prion (Other) / Protein-protein
interactions (Other)},
cin = {AG Jackson},
ddc = {570},
cid = {I:(DE-2719)1013019},
pnm = {899 - ohne Topic (POF4-899)},
pid = {G:(DE-HGF)POF4-899},
typ = {PUB:(DE-HGF)16},
pubmed = {pmid:39800229},
doi = {10.1016/j.nbd.2025.106802},
url = {https://pub.dzne.de/record/276154},
}