% IMPORTANT: The following is UTF-8 encoded.  This means that in the presence
% of non-ASCII characters, it will not work with BibTeX 0.99 or older.
% Instead, you should use an up-to-date BibTeX implementation like “bibtex8” or
% “biber”.

@ARTICLE{BaidoeAnsah:276278,
      author       = {Baidoe-Ansah, David and Mirzapourdelavar, Hadi and Aleshin,
                      Stepan and Schott, Björn Hendrik and Seidenbecher,
                      Constanze and Kaushik, Rahul and Dityatev, Alexander},
      title        = {{N}eurocan regulates axon initial segment organization and
                      neuronal activity.},
      journal      = {Matrix biology},
      volume       = {136},
      issn         = {0945-053X},
      address      = {Amsterdam [u.a.]},
      publisher    = {Elsevier},
      reportid     = {DZNE-2025-00257},
      pages        = {22 - 35},
      year         = {2025},
      abstract     = {The neural extracellular matrix (ECM) accumulates in the
                      form of perineuronal nets (PNNs), particularly around
                      fast-spiking GABAergic interneurons in the cortex and
                      hippocampus, but also around synapses and in association
                      with the axon initial segments (AIS) and nodes of Ranvier.
                      Increasing evidence highlights the role of Neurocan (Ncan),
                      a brain-specific component of ECM, in the pathophysiology of
                      neuropsychiatric disorders like bipolar disorder and
                      schizophrenia. Ncan localizes at PNNs, perisynaptically, and
                      at the nodes of Ranvier and the AIS, highlighting its
                      potential role in regulating axonal excitability. Here, we
                      used knockdown and knockout approaches in mouse primary
                      cortical neurons in combination with immunocytochemistry,
                      Western blotting and electrophysiological techniques to
                      characterize the role of Ncan in the organization of PNNs
                      and AISs and regulation of neuronal activity. We found that
                      reduced Ncan levels led to remodeling of PNNs around neurons
                      via upregulation of aggrecan mRNA and protein levels,
                      increased expression of activity-dependent c-Fos and FosB
                      genes and elevated spontaneous synaptic activity. The latter
                      correlated with increased levels of ankyrin-G in the AIS,
                      particularly in excitatory neurons, and with the elevated
                      expression of Nav1.6 channels. Our results suggest that Ncan
                      regulates the expression of key proteins in PNNs and AISs
                      and provide new insights into its role in fine-tuning
                      neuronal functions.},
      keywords     = {Animals / Mice / Axon Initial Segment: metabolism /
                      Neurocan: metabolism / Neurocan: genetics / Mice, Knockout /
                      NAV1.6 Voltage-Gated Sodium Channel: metabolism / NAV1.6
                      Voltage-Gated Sodium Channel: genetics / Ankyrins:
                      metabolism / Ankyrins: genetics / Neurons: metabolism /
                      Proto-Oncogene Proteins c-fos: genetics / Proto-Oncogene
                      Proteins c-fos: metabolism / Aggrecans: metabolism /
                      Aggrecans: genetics / Cells, Cultured / Extracellular
                      Matrix: metabolism / Axons: metabolism / Gene Expression
                      Regulation / Extracellular matrix (Other) / neurocan (Other)
                      / neuronal excitability (Other) / perineuronal net (Other) /
                      synaptic plasticity (Other)},
      cin          = {AG Dityatev / AG Fischer},
      ddc          = {570},
      cid          = {I:(DE-2719)1310007 / I:(DE-2719)1410002},
      pnm          = {351 - Brain Function (POF4-351) / 352 - Disease Mechanisms
                      (POF4-352)},
      pid          = {G:(DE-HGF)POF4-351 / G:(DE-HGF)POF4-352},
      typ          = {PUB:(DE-HGF)16},
      pubmed       = {pmid:39788215},
      doi          = {10.1016/j.matbio.2025.01.001},
      url          = {https://pub.dzne.de/record/276278},
}