Home > Publications Database > Biflavonoid Methylchamaejasmin and Khaya grandifoliola Extract Inhibit NLRP3 Inflammasome in THP-1 Cell Model of Neuroinflammation. > print

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005     20250204165928.0
024 7 _ |a 10.1007/s12035-024-04365-4
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024 7 _ |a 1559-1182
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037 _ _ |a DZNE-2025-00259
041 _ _ |a English
082 _ _ |a 570
100 1 _ |a Owona, Brice Ayissi
|0 0000-0002-2380-527X
|b 0
|e First author
245 _ _ |a Biflavonoid Methylchamaejasmin and Khaya grandifoliola Extract Inhibit NLRP3 Inflammasome in THP-1 Cell Model of Neuroinflammation.
260 _ _ |a Totowa, NJ
|c 2025
|b Humana Press
336 7 _ |a article
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520 _ _ |a Neuroinflammation is a common hallmark of Alzheimer's disease (AD), with NLRP3 inflammasome proven to be activated in microglia of AD patients' brains. In this study, a newly isolated biflavonoid (7,7'-di-O-methylchamaejasmin/M8) and a crude extract of the plant Khaya grandifoliola (KG) were investigated for their inhibitory effect on inflammasome activation. In preliminary experiments, M8 and KG showed no cytotoxicity on human macrophage-like differentiated THP-1 cells and exhibited anti-inflammatory inhibition of nitric oxide produced following lipopolysaccharide stimulation. Furthermore, M8 and KG blocked IL-1β and IL-18 production by reducing NLRP3 inflammasome components including NFκB, NLRP3, Caspase-1, pro-IL-1β, and pro-IL-18 at the mRNA and protein levels. Regarding the formation of ASC (apoptosis-associated speck-like protein containing a CARD) specks during inflammasome activation, the size and fluorescent intensity of the existing specks were unchanged across all treatment conditions. However, M8 and KG treatments were shown to prevent further speck formation. In addition, experiments on amyloid β phagocytosis showed that M8 and KG pretreatments can restore the phagocytic activity of THP-1 cells, which was impaired following inflammasome activation. Altogether, our findings describe for the first time a promising role of biflavonoids and KG extract in preventing inflammasome activation and protecting against neuroinflammation, a key factor in AD development.
536 _ _ |a 353 - Clinical and Health Care Research (POF4-353)
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588 _ _ |a Dataset connected to CrossRef, PubMed, , Journals: pub.dzne.de
650 _ 7 |a Khaya grandifoliola
|2 Other
650 _ 7 |a 7,7′-di-O-Methylchamaejasmin
|2 Other
650 _ 7 |a Biflavonoid
|2 Other
650 _ 7 |a NLRP3 inflammasome
|2 Other
650 _ 7 |a THP-1 cells
|2 Other
650 _ 7 |a NLR Family, Pyrin Domain-Containing 3 Protein
|2 NLM Chemicals
650 _ 7 |a Inflammasomes
|2 NLM Chemicals
650 _ 7 |a Plant Extracts
|2 NLM Chemicals
650 _ 7 |a Biflavonoids
|2 NLM Chemicals
650 _ 7 |a Amyloid beta-Peptides
|2 NLM Chemicals
650 _ 7 |a Lipopolysaccharides
|2 NLM Chemicals
650 _ 7 |a Nitric Oxide
|0 31C4KY9ESH
|2 NLM Chemicals
650 _ 7 |a Interleukin-1beta
|2 NLM Chemicals
650 _ 2 |a Humans
|2 MeSH
650 _ 2 |a NLR Family, Pyrin Domain-Containing 3 Protein: metabolism
|2 MeSH
650 _ 2 |a Inflammasomes: metabolism
|2 MeSH
650 _ 2 |a Inflammasomes: drug effects
|2 MeSH
650 _ 2 |a Plant Extracts: pharmacology
|2 MeSH
650 _ 2 |a THP-1 Cells
|2 MeSH
650 _ 2 |a Biflavonoids: pharmacology
|2 MeSH
650 _ 2 |a Biflavonoids: therapeutic use
|2 MeSH
650 _ 2 |a Neuroinflammatory Diseases: drug therapy
|2 MeSH
650 _ 2 |a Neuroinflammatory Diseases: metabolism
|2 MeSH
650 _ 2 |a Neuroinflammatory Diseases: pathology
|2 MeSH
650 _ 2 |a Amyloid beta-Peptides: metabolism
|2 MeSH
650 _ 2 |a Lipopolysaccharides: pharmacology
|2 MeSH
650 _ 2 |a Meliaceae: chemistry
|2 MeSH
650 _ 2 |a Phagocytosis: drug effects
|2 MeSH
650 _ 2 |a Nitric Oxide: metabolism
|2 MeSH
650 _ 2 |a Interleukin-1beta: metabolism
|2 MeSH
700 1 _ |a Mary, Arnaud
|0 0000-0002-6597-766X
|b 1
700 1 _ |a Messi, Angelique N
|0 0000-0002-9184-2080
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700 1 _ |a Ravichandran, Kishore Aravind
|0 P:(DE-2719)9002244
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700 1 _ |a Mbing, Josephine Ngo
|0 0000-0002-1427-979X
|b 4
700 1 _ |a Pegnyemb, Emmanuel
|0 0000-0001-5431-621X
|b 5
700 1 _ |a Moundipa, Paul F
|0 0000-0002-2694-5569
|b 6
700 1 _ |a Heneka, Michael
|0 P:(DE-2719)2000008
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|e Last author
773 _ _ |a 10.1007/s12035-024-04365-4
|g Vol. 62, no. 2, p. 1605 - 1619
|0 PERI:(DE-600)2079384-4
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|t Molecular neurobiology
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|y 2025
|x 0893-7648
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