000276472 001__ 276472
000276472 005__ 20250412100028.0
000276472 0247_ $$2doi$$a10.7554/eLife.87078
000276472 0247_ $$2altmetric$$aaltmetric:150873925
000276472 0247_ $$2pmid$$apmid:39898808
000276472 037__ $$aDZNE-2025-00294
000276472 041__ $$aEnglish
000276472 082__ $$a600
000276472 1001_ $$00000-0002-3344-8683$$aThome, Christian$$b0
000276472 245__ $$aLive imaging of excitable axonal microdomains in ankyrin-G-GFP mice
000276472 260__ $$aCambridge$$beLife Sciences Publications$$c2025
000276472 3367_ $$2DRIVER$$aarticle
000276472 3367_ $$2DataCite$$aOutput Types/Journal article
000276472 3367_ $$0PUB:(DE-HGF)16$$2PUB:(DE-HGF)$$aJournal Article$$bjournal$$mjournal$$s1738760063_16407
000276472 3367_ $$2BibTeX$$aARTICLE
000276472 3367_ $$2ORCID$$aJOURNAL_ARTICLE
000276472 3367_ $$00$$2EndNote$$aJournal Article
000276472 520__ $$aThe axon initial segment (AIS) constitutes not only the site of action potential initiation, but also a hub for activity-dependent modulation of output generation. Recent studies shedding light on AIS function used predominantly post-hoc approaches since no robust murine in vivo live reporters exist. Here, we introduce a reporter line in which the AIS is intrinsically labeled by an ankyrin-G-GFP fusion protein activated by Cre recombinase, tagging the native Ank3 gene. Using confocal, superresolution, and two-photon microscopy as well as whole-cell patch-clamp recordings in vitro, ex vivo, and in vivo, we confirm that the subcellular scaffold of the AIS and electrophysiological parameters of labeled cells remain unchanged. We further uncover rapid AIS remodeling following increased network activity in this model system, as well as highly reproducible in vivo labeling of AIS over weeks. This novel reporter line allows longitudinal studies of AIS modulation and plasticity in vivo in real-time and thus provides a unique approach to study subcellular plasticity in a broad range of applications.
000276472 536__ $$0G:(DE-HGF)POF4-351$$a351 - Brain Function (POF4-351)$$cPOF4-351$$fPOF IV$$x0
000276472 588__ $$aDataset connected to CrossRef, Journals: pub.dzne.de
000276472 650_7 $$2Other$$aAIS plasticity
000276472 650_7 $$2Other$$aankryin-G
000276472 650_7 $$2Other$$aaxon initial segment
000276472 650_7 $$2Other$$alive imaging
000276472 650_7 $$2Other$$amouse
000276472 650_7 $$2Other$$aneuroscience
000276472 650_7 $$2Other$$anode of Ranvier
000276472 650_7 $$2NLM Chemicals$$aAnkyrins
000276472 650_7 $$0147336-22-9$$2NLM Chemicals$$aGreen Fluorescent Proteins
000276472 650_7 $$2NLM Chemicals$$aAnk3 protein, mouse
000276472 650_2 $$2MeSH$$aAnimals
000276472 650_2 $$2MeSH$$aAnkyrins: metabolism
000276472 650_2 $$2MeSH$$aAnkyrins: genetics
000276472 650_2 $$2MeSH$$aMice
000276472 650_2 $$2MeSH$$aGreen Fluorescent Proteins: metabolism
000276472 650_2 $$2MeSH$$aGreen Fluorescent Proteins: genetics
000276472 650_2 $$2MeSH$$aAxons: physiology
000276472 650_2 $$2MeSH$$aAxons: metabolism
000276472 650_2 $$2MeSH$$aAxon Initial Segment: metabolism
000276472 650_2 $$2MeSH$$aPatch-Clamp Techniques
000276472 650_2 $$2MeSH$$aGenes, Reporter
000276472 650_2 $$2MeSH$$aMice, Transgenic
000276472 650_2 $$2MeSH$$aAction Potentials
000276472 7001_ $$aJanssen, Jan Maximilian$$b1
000276472 7001_ $$aKarabulut, Seda$$b2
000276472 7001_ $$aAcuna, Claudio$$b3
000276472 7001_ $$aD'Este, Elisa$$b4
000276472 7001_ $$aSoyka, Stella J$$b5
000276472 7001_ $$aBaum, Konrad$$b6
000276472 7001_ $$aBock, Michael$$b7
000276472 7001_ $$00000-0003-4801-3057$$aLehmann, Nadja$$b8
000276472 7001_ $$aRoos, Johannes$$b9
000276472 7001_ $$00000-0002-5592-3326$$aStevens, Nikolas A$$b10
000276472 7001_ $$0P:(DE-2719)9001582$$aHasegawa, Masashi$$b11
000276472 7001_ $$0P:(DE-2719)9001367$$aGanea, Dan Alin$$b12$$udzne
000276472 7001_ $$0P:(DE-2719)9001347$$aBenoit, Chloé M$$b13
000276472 7001_ $$0P:(DE-2719)9001219$$aGründemann, Jan$$b14
000276472 7001_ $$00000-0002-3977-1684$$aMin, Lia Y$$b15
000276472 7001_ $$aBird, Kalynn M$$b16
000276472 7001_ $$aSchultz, Christian$$b17
000276472 7001_ $$aBennett, Vann$$b18
000276472 7001_ $$00000-0002-4207-5823$$aJenkins, Paul$$b19
000276472 7001_ $$00000-0001-8020-6604$$aEngelhardt, Maren$$b20
000276472 773__ $$0PERI:(DE-600)2687154-3$$a10.7554/eLife.87078$$gVol. 12, p. RP87078$$pRP87078$$teLife$$v12$$x2050-084X$$y2025
000276472 8564_ $$uhttps://pub.dzne.de/record/276472/files/DZNE-2025-00294.pdf$$yOpenAccess
000276472 8564_ $$uhttps://pub.dzne.de/record/276472/files/DZNE-2025-00294.pdf?subformat=pdfa$$xpdfa$$yOpenAccess
000276472 909CO $$ooai:pub.dzne.de:276472$$popenaire$$popen_access$$pVDB$$pdriver$$pdnbdelivery
000276472 9101_ $$0I:(DE-588)1065079516$$6P:(DE-2719)9001582$$aDeutsches Zentrum für Neurodegenerative Erkrankungen$$b11$$kDZNE
000276472 9101_ $$0I:(DE-HGF)0$$6P:(DE-2719)9001367$$aExternal Institute$$b12$$kExtern
000276472 9101_ $$0I:(DE-588)1065079516$$6P:(DE-2719)9001347$$aDeutsches Zentrum für Neurodegenerative Erkrankungen$$b13$$kDZNE
000276472 9101_ $$0I:(DE-588)1065079516$$6P:(DE-2719)9001219$$aDeutsches Zentrum für Neurodegenerative Erkrankungen$$b14$$kDZNE
000276472 9131_ $$0G:(DE-HGF)POF4-351$$1G:(DE-HGF)POF4-350$$2G:(DE-HGF)POF4-300$$3G:(DE-HGF)POF4$$4G:(DE-HGF)POF$$aDE-HGF$$bGesundheit$$lNeurodegenerative Diseases$$vBrain Function$$x0
000276472 9141_ $$y2025
000276472 915__ $$0StatID:(DE-HGF)0200$$2StatID$$aDBCoverage$$bSCOPUS$$d2024-12-28
000276472 915__ $$0StatID:(DE-HGF)0160$$2StatID$$aDBCoverage$$bEssential Science Indicators$$d2024-12-28
000276472 915__ $$0StatID:(DE-HGF)1050$$2StatID$$aDBCoverage$$bBIOSIS Previews$$d2024-12-28
000276472 915__ $$0StatID:(DE-HGF)1190$$2StatID$$aDBCoverage$$bBiological Abstracts$$d2024-12-28
000276472 915__ $$0StatID:(DE-HGF)0600$$2StatID$$aDBCoverage$$bEbsco Academic Search$$d2024-12-28
000276472 915__ $$0StatID:(DE-HGF)1040$$2StatID$$aDBCoverage$$bZoological Record$$d2024-12-28
000276472 915__ $$0StatID:(DE-HGF)0100$$2StatID$$aJCR$$bELIFE : 2022$$d2024-12-28
000276472 915__ $$0StatID:(DE-HGF)0501$$2StatID$$aDBCoverage$$bDOAJ Seal$$d2024-07-30T13:58:16Z
000276472 915__ $$0StatID:(DE-HGF)0500$$2StatID$$aDBCoverage$$bDOAJ$$d2024-07-30T13:58:16Z
000276472 915__ $$0StatID:(DE-HGF)0113$$2StatID$$aWoS$$bScience Citation Index Expanded$$d2024-12-28
000276472 915__ $$0StatID:(DE-HGF)0700$$2StatID$$aFees$$d2024-12-28
000276472 915__ $$0StatID:(DE-HGF)0150$$2StatID$$aDBCoverage$$bWeb of Science Core Collection$$d2024-12-28
000276472 915__ $$0StatID:(DE-HGF)0510$$2StatID$$aOpenAccess
000276472 915__ $$0StatID:(DE-HGF)0030$$2StatID$$aPeer Review$$bASC$$d2024-12-28
000276472 915__ $$0StatID:(DE-HGF)0561$$2StatID$$aArticle Processing Charges$$d2024-12-28
000276472 915__ $$0StatID:(DE-HGF)9905$$2StatID$$aIF >= 5$$bELIFE : 2022$$d2024-12-28
000276472 915__ $$0StatID:(DE-HGF)0300$$2StatID$$aDBCoverage$$bMedline$$d2024-12-28
000276472 915__ $$0LIC:(DE-HGF)CCBY4$$2HGFVOC$$aCreative Commons Attribution CC BY 4.0
000276472 915__ $$0StatID:(DE-HGF)0199$$2StatID$$aDBCoverage$$bClarivate Analytics Master Journal List$$d2024-12-28
000276472 920__ $$lyes
000276472 9201_ $$0I:(DE-2719)5000069$$kAG Gründemann$$lNeural Circuit Computations$$x0
000276472 980__ $$ajournal
000276472 980__ $$aVDB
000276472 980__ $$aUNRESTRICTED
000276472 980__ $$aI:(DE-2719)5000069
000276472 9801_ $$aFullTexts