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@ARTICLE{Thome:276472,
      author       = {Thome, Christian and Janssen, Jan Maximilian and Karabulut,
                      Seda and Acuna, Claudio and D'Este, Elisa and Soyka, Stella
                      J and Baum, Konrad and Bock, Michael and Lehmann, Nadja and
                      Roos, Johannes and Stevens, Nikolas A and Hasegawa, Masashi
                      and Ganea, Dan Alin and Benoit, Chloé M and Gründemann,
                      Jan and Min, Lia Y and Bird, Kalynn M and Schultz, Christian
                      and Bennett, Vann and Jenkins, Paul and Engelhardt, Maren},
      title        = {{L}ive imaging of excitable axonal microdomains in
                      ankyrin-{G}-{GFP} mice},
      journal      = {eLife},
      volume       = {12},
      issn         = {2050-084X},
      address      = {Cambridge},
      publisher    = {eLife Sciences Publications},
      reportid     = {DZNE-2025-00294},
      pages        = {RP87078},
      year         = {2025},
      abstract     = {The axon initial segment (AIS) constitutes not only the
                      site of action potential initiation, but also a hub for
                      activity-dependent modulation of output generation. Recent
                      studies shedding light on AIS function used predominantly
                      post-hoc approaches since no robust murine in vivo live
                      reporters exist. Here, we introduce a reporter line in which
                      the AIS is intrinsically labeled by an ankyrin-G-GFP fusion
                      protein activated by Cre recombinase, tagging the native
                      Ank3 gene. Using confocal, superresolution, and two-photon
                      microscopy as well as whole-cell patch-clamp recordings in
                      vitro, ex vivo, and in vivo, we confirm that the subcellular
                      scaffold of the AIS and electrophysiological parameters of
                      labeled cells remain unchanged. We further uncover rapid AIS
                      remodeling following increased network activity in this
                      model system, as well as highly reproducible in vivo
                      labeling of AIS over weeks. This novel reporter line allows
                      longitudinal studies of AIS modulation and plasticity in
                      vivo in real-time and thus provides a unique approach to
                      study subcellular plasticity in a broad range of
                      applications.},
      keywords     = {Animals / Ankyrins: metabolism / Ankyrins: genetics / Mice
                      / Green Fluorescent Proteins: metabolism / Green Fluorescent
                      Proteins: genetics / Axons: physiology / Axons: metabolism /
                      Axon Initial Segment: metabolism / Patch-Clamp Techniques /
                      Genes, Reporter / Mice, Transgenic / Action Potentials / AIS
                      plasticity (Other) / ankryin-G (Other) / axon initial
                      segment (Other) / live imaging (Other) / mouse (Other) /
                      neuroscience (Other) / node of Ranvier (Other) / Ankyrins
                      (NLM Chemicals) / Green Fluorescent Proteins (NLM Chemicals)
                      / Ank3 protein, mouse (NLM Chemicals)},
      cin          = {AG Gründemann},
      ddc          = {600},
      cid          = {I:(DE-2719)5000069},
      pnm          = {351 - Brain Function (POF4-351)},
      pid          = {G:(DE-HGF)POF4-351},
      typ          = {PUB:(DE-HGF)16},
      pubmed       = {pmid:39898808},
      doi          = {10.7554/eLife.87078},
      url          = {https://pub.dzne.de/record/276472},
}