Home > Publications Database > Live imaging of excitable axonal microdomains in ankyrin-G-GFP mice > print

001     276472
005     20250412100028.0
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100 1 _ |a Thome, Christian
|0 0000-0002-3344-8683
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245 _ _ |a Live imaging of excitable axonal microdomains in ankyrin-G-GFP mice
260 _ _ |a Cambridge
|c 2025
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520 _ _ |a The axon initial segment (AIS) constitutes not only the site of action potential initiation, but also a hub for activity-dependent modulation of output generation. Recent studies shedding light on AIS function used predominantly post-hoc approaches since no robust murine in vivo live reporters exist. Here, we introduce a reporter line in which the AIS is intrinsically labeled by an ankyrin-G-GFP fusion protein activated by Cre recombinase, tagging the native Ank3 gene. Using confocal, superresolution, and two-photon microscopy as well as whole-cell patch-clamp recordings in vitro, ex vivo, and in vivo, we confirm that the subcellular scaffold of the AIS and electrophysiological parameters of labeled cells remain unchanged. We further uncover rapid AIS remodeling following increased network activity in this model system, as well as highly reproducible in vivo labeling of AIS over weeks. This novel reporter line allows longitudinal studies of AIS modulation and plasticity in vivo in real-time and thus provides a unique approach to study subcellular plasticity in a broad range of applications.
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650 _ 7 |a AIS plasticity
|2 Other
650 _ 7 |a ankryin-G
|2 Other
650 _ 7 |a axon initial segment
|2 Other
650 _ 7 |a live imaging
|2 Other
650 _ 7 |a mouse
|2 Other
650 _ 7 |a neuroscience
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650 _ 7 |a node of Ranvier
|2 Other
650 _ 7 |a Ankyrins
|2 NLM Chemicals
650 _ 7 |a Green Fluorescent Proteins
|0 147336-22-9
|2 NLM Chemicals
650 _ 7 |a Ank3 protein, mouse
|2 NLM Chemicals
650 _ 2 |a Animals
|2 MeSH
650 _ 2 |a Ankyrins: metabolism
|2 MeSH
650 _ 2 |a Ankyrins: genetics
|2 MeSH
650 _ 2 |a Mice
|2 MeSH
650 _ 2 |a Green Fluorescent Proteins: metabolism
|2 MeSH
650 _ 2 |a Green Fluorescent Proteins: genetics
|2 MeSH
650 _ 2 |a Axons: physiology
|2 MeSH
650 _ 2 |a Axons: metabolism
|2 MeSH
650 _ 2 |a Axon Initial Segment: metabolism
|2 MeSH
650 _ 2 |a Patch-Clamp Techniques
|2 MeSH
650 _ 2 |a Genes, Reporter
|2 MeSH
650 _ 2 |a Mice, Transgenic
|2 MeSH
650 _ 2 |a Action Potentials
|2 MeSH
700 1 _ |a Janssen, Jan Maximilian
|b 1
700 1 _ |a Karabulut, Seda
|b 2
700 1 _ |a Acuna, Claudio
|b 3
700 1 _ |a D'Este, Elisa
|b 4
700 1 _ |a Soyka, Stella J
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700 1 _ |a Baum, Konrad
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700 1 _ |a Bock, Michael
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700 1 _ |a Lehmann, Nadja
|0 0000-0003-4801-3057
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700 1 _ |a Roos, Johannes
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700 1 _ |a Stevens, Nikolas A
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700 1 _ |a Hasegawa, Masashi
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700 1 _ |a Ganea, Dan Alin
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700 1 _ |a Benoit, Chloé M
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700 1 _ |a Gründemann, Jan
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700 1 _ |a Min, Lia Y
|0 0000-0002-3977-1684
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700 1 _ |a Bird, Kalynn M
|b 16
700 1 _ |a Schultz, Christian
|b 17
700 1 _ |a Bennett, Vann
|b 18
700 1 _ |a Jenkins, Paul
|0 0000-0002-4207-5823
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700 1 _ |a Engelhardt, Maren
|0 0000-0001-8020-6604
|b 20
773 _ _ |a 10.7554/eLife.87078
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