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@ARTICLE{Beckrge:276481,
      author       = {Beckröge, Tobias and Jux, Bettina and Seifert, Hannah and
                      Theobald, Hannah and De Domenico, Elena and Paulusch, Stefan
                      and Beyer, Marc and Schlitzer, Andreas and Mass, Elvira and
                      Kolanus, Waldemar},
      title        = {{I}mpaired primitive erythropoiesis and defective vascular
                      development in {T}rim71-{KO} embryos.},
      journal      = {Life science alliance},
      volume       = {8},
      number       = {4},
      issn         = {2575-1077},
      address      = {Heidelberg},
      publisher    = {EMBO Press},
      reportid     = {DZNE-2025-00300},
      pages        = {e202402956},
      year         = {2025},
      abstract     = {The transition of an embryo from gastrulation to
                      organogenesis requires precisely coordinated changes in gene
                      expression, but the underlying mechanisms remain unclear.
                      The RNA-binding protein Trim71 is essential for development
                      and serves as a potent regulator of post-transcriptional
                      gene expression. Here, we show that global deficiency of
                      Trim71 induces severe defects in mesoderm-derived cells at
                      the onset of organogenesis. Murine Trim71-KO embryos
                      displayed impaired primitive erythropoiesis, yolk sac
                      vasculature, heart function, and circulation, explaining the
                      embryonic lethality of these mice. Tie2 Cre Trim71
                      conditional knockout did not induce strong defects, showing
                      that Trim71 expression in endothelial cells and their
                      immediate progenitors is dispensable for embryonic survival.
                      scRNA-seq of E7.5 global Trim71-KO embryos revealed that
                      transcriptomic changes arise already at gastrulation,
                      showing a strong up-regulation of the mesodermal pioneer
                      transcription factor Eomes. We identify Eomes as a direct
                      target of Trim71-mediated mRNA repression via the NHL
                      domain, demonstrating a functional link between these
                      important regulatory genes. Taken together, our data suggest
                      that Trim71-dependent control of gene expression at
                      gastrulation establishes a framework for proper development
                      during organogenesis.},
      keywords     = {Animals / Erythropoiesis: genetics / Mice / Mice, Knockout
                      / Gene Expression Regulation, Developmental: genetics /
                      Embryo, Mammalian: metabolism / Organogenesis: genetics /
                      Yolk Sac: metabolism / Yolk Sac: blood supply / Yolk Sac:
                      embryology / T-Box Domain Proteins: genetics / T-Box Domain
                      Proteins: metabolism / Gastrulation: genetics / Endothelial
                      Cells: metabolism / Female / RNA-Binding Proteins:
                      metabolism / RNA-Binding Proteins: genetics / Mesoderm:
                      metabolism / Mesoderm: embryology / T-Box Domain Proteins
                      (NLM Chemicals) / RNA-Binding Proteins (NLM Chemicals)},
      cin          = {AG Schultze / PRECISE / AG Beyer},
      ddc          = {570},
      cid          = {I:(DE-2719)1013038 / I:(DE-2719)1013031 /
                      I:(DE-2719)1013035},
      pnm          = {354 - Disease Prevention and Healthy Aging (POF4-354) / 352
                      - Disease Mechanisms (POF4-352) / 351 - Brain Function
                      (POF4-351)},
      pid          = {G:(DE-HGF)POF4-354 / G:(DE-HGF)POF4-352 /
                      G:(DE-HGF)POF4-351},
      typ          = {PUB:(DE-HGF)16},
      pubmed       = {pmid:39909558},
      doi          = {10.26508/lsa.202402956},
      url          = {https://pub.dzne.de/record/276481},
}