000276751 001__ 276751
000276751 005__ 20250213170036.0
000276751 020__ $$a978-3-031-76549-0 (print)
000276751 020__ $$a978-3-031-76550-6 (electronic)
000276751 0247_ $$2doi$$a10.1007/978-3-031-76550-6_63
000276751 0247_ $$2pmid$$apmid:39930226
000276751 0247_ $$2ISSN$$a0065-2598
000276751 0247_ $$2ISSN$$a2214-8019
000276751 037__ $$aDZNE-2025-00309
000276751 041__ $$aEnglish
000276751 082__ $$a570
000276751 1001_ $$0P:(DE-2719)2811441$$aWagner, Felix$$b0$$eFirst author$$udzne
000276751 1112_ $$aInternational Workshop on Ambient Assisted Living$$cTorremolinos$$d2011-06-08 - 2011-06-10$$gIWAAL 2011$$wSpain
000276751 245__ $$aLive Imaging Microscopy of Human Retina Organoids: Photoreceptor Pathology.
000276751 260__ $$aCham$$bSpringer Nature Switzerland$$c2025
000276751 29510 $$aRetinal Degenerative Diseases XX / Bowes Rickman, Catherine (Editor) ; Cham : Springer Nature Switzerland, 2025, Chapter 63 ; ISSN: 0065-2598=2214-8019 ; ISBN: 978-3-031-76549-0=978-3-031-76550-6 ; doi:10.1007/978-3-031-76550-6
000276751 300__ $$a383 - 387
000276751 3367_ $$2ORCID$$aCONFERENCE_PAPER
000276751 3367_ $$033$$2EndNote$$aConference Paper
000276751 3367_ $$2BibTeX$$aINPROCEEDINGS
000276751 3367_ $$2DRIVER$$aconferenceObject
000276751 3367_ $$2DataCite$$aOutput Types/Conference Paper
000276751 3367_ $$0PUB:(DE-HGF)8$$2PUB:(DE-HGF)$$aContribution to a conference proceedings$$bcontrib$$mcontrib$$s1739454247_21758
000276751 3367_ $$0PUB:(DE-HGF)7$$2PUB:(DE-HGF)$$aContribution to a book$$mcontb
000276751 4900_ $$aAdvances in Experimental Medicine and Biology$$v1468
000276751 520__ $$aNeurodegenerative diseases involve many different (sub)cellular pathologic processes like mitochondria damage, which are still incompletely understood and present future targets for therapy development. Live imaging microscopy of dynamic pathologic changes at single cell resolution might advance studies of pathomechanisms and biomarkers. Organoid technologies may complement and facilitate animal and patient studies: We recently reported a novel pathomechanism of photoreceptor degeneration via cell extrusion, identified and validated by live imaging in human retina organoids. Here, we describe the related workflow for live imaging organoid studies. As another use-case, we describe mitochondrial alterations in photoreceptors acutely damaged by the ionophore CCCP. Our data further support a CCCP-induced experimental model for photoreceptor pathology, which can be monitored by JC-1 live dye-assisted live imaging. Thus, we demonstrate that live imaging advances studies of early subcellular pathologic events in human organoids. Such approaches might facilitate future therapeutic target identification and development for early intervention.
000276751 536__ $$0G:(DE-HGF)POF4-352$$a352 - Disease Mechanisms (POF4-352)$$cPOF4-352$$fPOF IV$$x0
000276751 588__ $$aDataset connected to CrossRef Book Series, PubMed, , Journals: pub.dzne.de
000276751 650_7 $$2Other$$aHuman
000276751 650_7 $$2Other$$aLive imaging
000276751 650_7 $$2Other$$aMitochondria
000276751 650_7 $$2Other$$aNeurodegeneration
000276751 650_7 $$2Other$$aOrganoid
000276751 650_7 $$2Other$$aRetina
000276751 650_2 $$2MeSH$$aOrganoids: pathology
000276751 650_2 $$2MeSH$$aOrganoids: metabolism
000276751 650_2 $$2MeSH$$aHumans
000276751 650_2 $$2MeSH$$aPhotoreceptor Cells, Vertebrate: pathology
000276751 650_2 $$2MeSH$$aPhotoreceptor Cells, Vertebrate: metabolism
000276751 650_2 $$2MeSH$$aRetina: pathology
000276751 650_2 $$2MeSH$$aRetina: metabolism
000276751 650_2 $$2MeSH$$aMitochondria: metabolism
000276751 650_2 $$2MeSH$$aMitochondria: pathology
000276751 650_2 $$2MeSH$$aRetinal Degeneration: pathology
000276751 650_2 $$2MeSH$$aRetinal Degeneration: metabolism
000276751 7001_ $$0P:(DE-2719)2000041$$aKarl, Mike O$$b1$$eLast author$$udzne
000276751 773__ $$a10.1007/978-3-031-76550-6_63
000276751 909CO $$ooai:pub.dzne.de:276751$$pVDB
000276751 9101_ $$0I:(DE-588)1065079516$$6P:(DE-2719)2811441$$aDeutsches Zentrum für Neurodegenerative Erkrankungen$$b0$$kDZNE
000276751 9101_ $$0I:(DE-588)1065079516$$6P:(DE-2719)2000041$$aDeutsches Zentrum für Neurodegenerative Erkrankungen$$b1$$kDZNE
000276751 9131_ $$0G:(DE-HGF)POF4-352$$1G:(DE-HGF)POF4-350$$2G:(DE-HGF)POF4-300$$3G:(DE-HGF)POF4$$4G:(DE-HGF)POF$$aDE-HGF$$bGesundheit$$lNeurodegenerative Diseases$$vDisease Mechanisms$$x0
000276751 9141_ $$y2025
000276751 915__ $$0StatID:(DE-HGF)0200$$2StatID$$aDBCoverage$$bSCOPUS$$d2024-12-06
000276751 915__ $$0StatID:(DE-HGF)0300$$2StatID$$aDBCoverage$$bMedline$$d2024-12-06
000276751 9201_ $$0I:(DE-2719)1710004$$kAG Karl$$lRetina Regeneration and Degeneration$$x0
000276751 980__ $$acontrib
000276751 980__ $$aVDB
000276751 980__ $$acontb
000276751 980__ $$aI:(DE-2719)1710004
000276751 980__ $$aUNRESTRICTED