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@INPROCEEDINGS{Wagner:276751,
author = {Wagner, Felix and Karl, Mike O},
title = {{L}ive {I}maging {M}icroscopy of {H}uman {R}etina
{O}rganoids: {P}hotoreceptor {P}athology.},
volume = {1468},
address = {Cham},
publisher = {Springer Nature Switzerland},
reportid = {DZNE-2025-00309},
isbn = {978-3-031-76549-0 (print)},
series = {Advances in Experimental Medicine and Biology},
pages = {383 - 387},
year = {2025},
comment = {Retinal Degenerative Diseases XX / Bowes Rickman, Catherine
(Editor) ; Cham : Springer Nature Switzerland, 2025, Chapter
63 ; ISSN: 0065-2598=2214-8019 ; ISBN:
978-3-031-76549-0=978-3-031-76550-6 ;
doi:10.1007/978-3-031-76550-6},
booktitle = {Retinal Degenerative Diseases XX /
Bowes Rickman, Catherine (Editor) ;
Cham : Springer Nature Switzerland,
2025, Chapter 63 ; ISSN:
0065-2598=2214-8019 ; ISBN:
978-3-031-76549-0=978-3-031-76550-6 ;
doi:10.1007/978-3-031-76550-6},
abstract = {Neurodegenerative diseases involve many different
(sub)cellular pathologic processes like mitochondria damage,
which are still incompletely understood and present future
targets for therapy development. Live imaging microscopy of
dynamic pathologic changes at single cell resolution might
advance studies of pathomechanisms and biomarkers. Organoid
technologies may complement and facilitate animal and
patient studies: We recently reported a novel pathomechanism
of photoreceptor degeneration via cell extrusion, identified
and validated by live imaging in human retina organoids.
Here, we describe the related workflow for live imaging
organoid studies. As another use-case, we describe
mitochondrial alterations in photoreceptors acutely damaged
by the ionophore CCCP. Our data further support a
CCCP-induced experimental model for photoreceptor pathology,
which can be monitored by JC-1 live dye-assisted live
imaging. Thus, we demonstrate that live imaging advances
studies of early subcellular pathologic events in human
organoids. Such approaches might facilitate future
therapeutic target identification and development for early
intervention.},
month = {Jun},
date = {2011-06-08},
organization = {International Workshop on Ambient
Assisted Living, Torremolinos (Spain),
8 Jun 2011 - 10 Jun 2011},
keywords = {Organoids: pathology / Organoids: metabolism / Humans /
Photoreceptor Cells, Vertebrate: pathology / Photoreceptor
Cells, Vertebrate: metabolism / Retina: pathology / Retina:
metabolism / Mitochondria: metabolism / Mitochondria:
pathology / Retinal Degeneration: pathology / Retinal
Degeneration: metabolism / Human (Other) / Live imaging
(Other) / Mitochondria (Other) / Neurodegeneration (Other) /
Organoid (Other) / Retina (Other)},
cin = {AG Karl},
ddc = {570},
cid = {I:(DE-2719)1710004},
pnm = {352 - Disease Mechanisms (POF4-352)},
pid = {G:(DE-HGF)POF4-352},
typ = {PUB:(DE-HGF)8 / PUB:(DE-HGF)7},
pubmed = {pmid:39930226},
doi = {10.1007/978-3-031-76550-6_63},
url = {https://pub.dzne.de/record/276751},
}