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@ARTICLE{Entenmann:276752,
author = {Entenmann, Christian J and von Bronewski, Emily J and
Waldmann, Lilly and Meyer, Lea and Kersting, Katharina and
Roolfs, Laurens T and Schleker, Lasse M and Nieminen-Kelhä,
Melina and Kremenetskaia, Irina and Heppner, Frank L and
Fehlings, Michael G and Vajkoczy, Peter and Hubertus,
Vanessa},
title = {{A}nalysis of the spatiotemporal dynamics of vascular
injury and regeneration following experimental {S}pinal
{C}ord {I}njury.},
journal = {Brain and spine},
volume = {5},
issn = {2772-5294},
address = {[Amsterdam]},
publisher = {Elsevier B.V.},
reportid = {DZNE-2025-00310},
pages = {104191},
year = {2025},
abstract = {The loss of vasculature in Spinal Cord Injury (SCI)
contributes to secondary injury, expanding the injury to
unharmed spinal cord (SC) regions. Understanding these
mechanisms is crucial for developing therapeutic
interventions.Comprehensive analysis of the temporospatial
dynamics of vascular injury and regeneration following
SCI.Adult C57BL/6J mice were subjected to clip-compression
SCI (Th 6/7, 5g, 60s, n = 20) or sham injury (laminectomy, n
= 4), and sacrificed at 1, 3, 7, 14, and 28 days (d)
post-injury following intracardial fluorescein
isothiocyanate (FITC)-Lectin perfusion. Histological
analysis (CD31, FITC-Lectin, Ki-67, IgG, TER-119) assessed
vascular changes, permeability, and proliferation within the
injury epicenter (region 0 (R0), ± 0,5 mm) and two adjacent
SC regions (R1: ± 1 mm, R2: ± 2.5 mm).Perfusion loss
(FITC-Lectin+/CD31+), was most severe in R0 and R1 at d3 (p
< 0.01). Significant vascular loss in R2 started at d3 (p =
0.043). Perfusion was restored at d28 in R0 and R1, and at
d7 in R2. Vessel density (CD31+) returned to baseline
quicker (R0: d3, R1 and R2: d14). Vascular proliferation
(CD31+/Ki-67+) manifested across all regions at d3 (p <
0.01), and most notably in R2 (p < 0.01). Vascular
permeability for IgG remained disrupted until d3 in R0 and
R1 and until d14 in R2.Vascular injury is most severe
initially and spreads to the surrounding SC regions. Gradual
vascular regeneration occurs early and up to a considerable
distance from the injury epicenter, highlighting the
potential of early therapeutic interventions targeted at
vascular repair and regeneration.},
keywords = {Angiogenesis (Other) / Blood spinal cord barrier (Other) /
Revascularization (Other) / Spinal cord injury (Other) /
Vascular injury (Other) / Vascular proliferation (Other)},
cin = {AG Heppner},
ddc = {610},
cid = {I:(DE-2719)1810007},
pnm = {353 - Clinical and Health Care Research (POF4-353)},
pid = {G:(DE-HGF)POF4-353},
typ = {PUB:(DE-HGF)16},
pubmed = {pmid:39935529},
pmc = {pmc:PMC11810697},
doi = {10.1016/j.bas.2025.104191},
url = {https://pub.dzne.de/record/276752},
}