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@ARTICLE{Tutas:277313,
author = {Tutas, Janine and Tolve, Marianna and Özer-Yildiz, Ebru
and Ickert, Lotte and Klein, Ines and Silverman, Quinn and
Liebsch, Filip and Dethloff, Frederik and Giavalisco,
Patrick and Endepols, Heike and Georgomanolis, Theodoros and
Neumaier, Bernd and Drzezga, Alexander and Schwarz, Guenter
and Thorens, Bernard and Gatto, Graziana and Frezza,
Christian and Kononenko, Natalia L},
title = {{A}utophagy regulator {ATG}5 preserves cerebellar function
by safeguarding its glycolytic activity.},
journal = {Nature metabolism},
volume = {7},
number = {2},
issn = {2522-5812},
address = {[London]},
publisher = {Springer Nature},
reportid = {DZNE-2025-00376},
pages = {297 - 320},
year = {2025},
abstract = {Dysfunctions in autophagy, a cellular mechanism for
breaking down components within lysosomes, often lead to
neurodegeneration. The specific mechanisms underlying
neuronal vulnerability due to autophagy dysfunction remain
elusive. Here we show that autophagy contributes to
cerebellar Purkinje cell (PC) survival by safeguarding their
glycolytic activity. Outside the conventional housekeeping
role, autophagy is also involved in the ATG5-mediated
regulation of glucose transporter 2 (GLUT2) levels during
cerebellar maturation. Autophagy-deficient PCs exhibit GLUT2
accumulation on the plasma membrane, along with increased
glucose uptake and alterations in glycolysis. We identify
lysophosphatidic acid and serine as glycolytic intermediates
that trigger PC death and demonstrate that the deletion of
GLUT2 in ATG5-deficient mice mitigates PC neurodegeneration
and rescues their ataxic gait. Taken together, this work
reveals a mechanism for regulating GLUT2 levels in neurons
and provides insights into the neuroprotective role of
autophagy by controlling glucose homeostasis in the brain.},
keywords = {Animals / Autophagy-Related Protein 5: metabolism /
Autophagy-Related Protein 5: genetics / Mice / Glycolysis /
Autophagy / Cerebellum: metabolism / Purkinje Cells:
metabolism / Glucose: metabolism / Glucose Transporter Type
2: metabolism / Mice, Knockout / Autophagy-Related Protein 5
(NLM Chemicals) / Atg5 protein, mouse (NLM Chemicals) /
Glucose (NLM Chemicals) / Glucose Transporter Type 2 (NLM
Chemicals) / Slc2a2 protein, mouse (NLM Chemicals)},
cin = {AG Boecker},
ddc = {610},
cid = {I:(DE-2719)1011202},
pnm = {353 - Clinical and Health Care Research (POF4-353)},
pid = {G:(DE-HGF)POF4-353},
typ = {PUB:(DE-HGF)16},
pubmed = {pmid:39815080},
doi = {10.1038/s42255-024-01196-4},
url = {https://pub.dzne.de/record/277313},
}