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000277421 037__ $$aDZNE-2025-00404
000277421 041__ $$aEnglish
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000277421 1001_ $$00000-0002-6703-7997$$aKardell, Oliver$$b0
000277421 245__ $$aMulticenter Longitudinal Quality Assessment of MS-Based Proteomics in Plasma and Serum.
000277421 260__ $$aWashington, DC$$bACS Publications$$c2025
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000277421 520__ $$aAdvancing MS-based proteomics toward clinical applications evolves around developing standardized start-to-finish and fit-for-purpose workflows for clinical specimens. Steps along the method design involve the determination and optimization of several bioanalytical parameters such as selectivity, sensitivity, accuracy, and precision. In a joint effort, eight proteomics laboratories belonging to the MSCoreSys initiative including the CLINSPECT-M, MSTARS, DIASyM, and SMART-CARE consortia performed a longitudinal round-robin study to assess the analysis performance of plasma and serum as clinically relevant samples. A variety of LC-MS/MS setups including mass spectrometer models from ThermoFisher and Bruker as well as LC systems from ThermoFisher, Evosep, and Waters Corporation were used in this study. As key performance indicators, sensitivity, precision, and reproducibility were monitored over time. Protein identifications range between 300 and 400 IDs across different state-of-the-art MS instruments, with timsTOF Pro, Orbitrap Exploris 480, and Q Exactive HF-X being among the top performers. Overall, 71 proteins are reproducibly detectable in all setups in both serum and plasma samples, and 22 of these proteins are FDA-approved biomarkers, which are reproducibly quantified (CV < 20% with label-free quantification). In total, the round-robin study highlights a promising baseline for bringing MS-based measurements of serum and plasma samples closer to clinical utility.
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000277421 650_7 $$2Other$$aLC-MS/MS
000277421 650_7 $$2Other$$aR package mpwR
000277421 650_7 $$2Other$$aclinical specimen
000277421 650_7 $$2Other$$alongitudinal round-robin study
000277421 650_7 $$2Other$$aplasma
000277421 650_7 $$2Other$$aserum
000277421 650_7 $$2NLM Chemicals$$aBiomarkers
000277421 650_7 $$2NLM Chemicals$$aBlood Proteins
000277421 650_2 $$2MeSH$$aHumans
000277421 650_2 $$2MeSH$$aProteomics: methods
000277421 650_2 $$2MeSH$$aProteomics: standards
000277421 650_2 $$2MeSH$$aTandem Mass Spectrometry: standards
000277421 650_2 $$2MeSH$$aTandem Mass Spectrometry: methods
000277421 650_2 $$2MeSH$$aLongitudinal Studies
000277421 650_2 $$2MeSH$$aChromatography, Liquid: methods
000277421 650_2 $$2MeSH$$aReproducibility of Results
000277421 650_2 $$2MeSH$$aBiomarkers: blood
000277421 650_2 $$2MeSH$$aBlood Proteins: analysis
000277421 650_2 $$2MeSH$$aPlasma: chemistry
000277421 650_2 $$2MeSH$$aSerum: chemistry
000277421 7001_ $$aGronauer, Thomas$$b1
000277421 7001_ $$avon Toerne, Christine$$b2
000277421 7001_ $$aMerl-Pham, Juliane$$b3
000277421 7001_ $$aKönig, Ann-Christine$$b4
000277421 7001_ $$aBarth, Teresa K$$b5
000277421 7001_ $$aMergner, Julia$$b6
000277421 7001_ $$00000-0002-6131-7322$$aLudwig, Christina$$b7
000277421 7001_ $$aTüshaus, Johanna$$b8
000277421 7001_ $$0P:(DE-2719)9001718$$aGiesbertz, Pieter$$b9$$udzne
000277421 7001_ $$0P:(DE-2719)9001161$$aBreimann, Stephan$$b10
000277421 7001_ $$aSchweizer, Lisa$$b11
000277421 7001_ $$aMüller, Torsten$$b12
000277421 7001_ $$aKliewer, Georg$$b13
000277421 7001_ $$00000-0002-8031-6384$$aDistler, Ute$$b14
000277421 7001_ $$00000-0002-9467-1213$$aGomez-Zepeda, David$$b15
000277421 7001_ $$aPopp, Oliver$$b16
000277421 7001_ $$aQin, Di$$b17
000277421 7001_ $$aTeupser, Daniel$$b18
000277421 7001_ $$00000-0001-8597-205X$$aCox, Jürgen$$b19
000277421 7001_ $$00000-0003-2993-8249$$aImhof, Axel$$b20
000277421 7001_ $$00000-0002-9094-1677$$aKüster, Bernhard$$b21
000277421 7001_ $$0P:(DE-2719)2181459$$aLichtenthaler, Stefan F$$b22$$udzne
000277421 7001_ $$aKrijgsveld, Jeroen$$b23
000277421 7001_ $$00000-0003-3034-0017$$aTenzer, Stefan$$b24
000277421 7001_ $$aMertins, Philipp$$b25
000277421 7001_ $$00000-0002-2244-5081$$aCoscia, Fabian$$b26
000277421 7001_ $$00000-0002-1630-6827$$aHauck, Stefanie M$$b27
000277421 773__ $$0PERI:(DE-600)2065254-9$$a10.1021/acs.jproteome.4c00644$$gVol. 24, no. 3, p. 1017 - 1029$$n3$$p1017 - 1029$$tJournal of proteome research$$v24$$x1535-3893$$y2025
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