% IMPORTANT: The following is UTF-8 encoded.  This means that in the presence
% of non-ASCII characters, it will not work with BibTeX 0.99 or older.
% Instead, you should use an up-to-date BibTeX implementation like “bibtex8” or
% “biber”.

@ARTICLE{Kardell:277421,
      author       = {Kardell, Oliver and Gronauer, Thomas and von Toerne,
                      Christine and Merl-Pham, Juliane and König, Ann-Christine
                      and Barth, Teresa K and Mergner, Julia and Ludwig, Christina
                      and Tüshaus, Johanna and Giesbertz, Pieter and Breimann,
                      Stephan and Schweizer, Lisa and Müller, Torsten and
                      Kliewer, Georg and Distler, Ute and Gomez-Zepeda, David and
                      Popp, Oliver and Qin, Di and Teupser, Daniel and Cox,
                      Jürgen and Imhof, Axel and Küster, Bernhard and
                      Lichtenthaler, Stefan F and Krijgsveld, Jeroen and Tenzer,
                      Stefan and Mertins, Philipp and Coscia, Fabian and Hauck,
                      Stefanie M},
      title        = {{M}ulticenter {L}ongitudinal {Q}uality {A}ssessment of
                      {MS}-{B}ased {P}roteomics in {P}lasma and {S}erum.},
      journal      = {Journal of proteome research},
      volume       = {24},
      number       = {3},
      issn         = {1535-3893},
      address      = {Washington, DC},
      publisher    = {ACS Publications},
      reportid     = {DZNE-2025-00404},
      pages        = {1017 - 1029},
      year         = {2025},
      abstract     = {Advancing MS-based proteomics toward clinical applications
                      evolves around developing standardized start-to-finish and
                      fit-for-purpose workflows for clinical specimens. Steps
                      along the method design involve the determination and
                      optimization of several bioanalytical parameters such as
                      selectivity, sensitivity, accuracy, and precision. In a
                      joint effort, eight proteomics laboratories belonging to the
                      MSCoreSys initiative including the CLINSPECT-M, MSTARS,
                      DIASyM, and SMART-CARE consortia performed a longitudinal
                      round-robin study to assess the analysis performance of
                      plasma and serum as clinically relevant samples. A variety
                      of LC-MS/MS setups including mass spectrometer models from
                      ThermoFisher and Bruker as well as LC systems from
                      ThermoFisher, Evosep, and Waters Corporation were used in
                      this study. As key performance indicators, sensitivity,
                      precision, and reproducibility were monitored over time.
                      Protein identifications range between 300 and 400 IDs across
                      different state-of-the-art MS instruments, with timsTOF Pro,
                      Orbitrap Exploris 480, and Q Exactive HF-X being among the
                      top performers. Overall, 71 proteins are reproducibly
                      detectable in all setups in both serum and plasma samples,
                      and 22 of these proteins are FDA-approved biomarkers, which
                      are reproducibly quantified (CV < $20\%$ with label-free
                      quantification). In total, the round-robin study highlights
                      a promising baseline for bringing MS-based measurements of
                      serum and plasma samples closer to clinical utility.},
      keywords     = {Humans / Proteomics: methods / Proteomics: standards /
                      Tandem Mass Spectrometry: standards / Tandem Mass
                      Spectrometry: methods / Longitudinal Studies /
                      Chromatography, Liquid: methods / Reproducibility of Results
                      / Biomarkers: blood / Blood Proteins: analysis / Plasma:
                      chemistry / Serum: chemistry / LC-MS/MS (Other) / R package
                      mpwR (Other) / clinical specimen (Other) / longitudinal
                      round-robin study (Other) / plasma (Other) / serum (Other) /
                      Biomarkers (NLM Chemicals) / Blood Proteins (NLM Chemicals)},
      cin          = {AG Lichtenthaler / AG Steiner},
      ddc          = {540},
      cid          = {I:(DE-2719)1110006 / I:(DE-2719)1110000-1},
      pnm          = {352 - Disease Mechanisms (POF4-352)},
      pid          = {G:(DE-HGF)POF4-352},
      typ          = {PUB:(DE-HGF)16},
      pubmed       = {pmid:39918541},
      doi          = {10.1021/acs.jproteome.4c00644},
      url          = {https://pub.dzne.de/record/277421},
}