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@ARTICLE{Mangiarotti:277728,
author = {Mangiarotti, Agustin and Sabri, Elias and Schmidt, Kita
Valerie and Hoffmann, Christian and Milovanovic, Dragomir
and Lipowsky, Reinhard and Dimova, Rumiana},
title = {{L}ipid packing and cholesterol content regulate membrane
wetting and remodeling by biomolecular condensates.},
journal = {Nature Communications},
volume = {16},
number = {1},
issn = {2041-1723},
address = {[London]},
publisher = {Springer Nature},
reportid = {DZNE-2025-00449},
pages = {2756},
year = {2025},
abstract = {Biomolecular condensates play a central role in cellular
processes by interacting with membranes driving wetting
transitions and inducing mutual remodeling. While
condensates are known to locally alter membrane properties
such as lipid packing and hydration, it remains unclear how
membrane composition and phase state in turn affect
condensate affinity. Here, we show that it is not only the
membrane phase itself, but rather the degree of lipid
packing that determines the condensate affinity for
membranes. Increasing lipid chain length, saturation, or
cholesterol content, enhances lipid packing, thereby
decreasing condensate interaction. This regulatory mechanism
is consistent across various condensate-membrane systems,
highlighting the critical role of the membrane interface. In
addition, protein adsorption promotes extensive membrane
remodeling, including the formation of tubes and
double-membrane sheets. Our findings reveal a mechanism by
which membrane composition fine-tunes condensate wetting,
highlighting its potential impact on cellular functions and
organelle interactions.},
keywords = {Cholesterol: metabolism / Cholesterol: chemistry /
Wettability / Biomolecular Condensates: metabolism /
Biomolecular Condensates: chemistry / Cell Membrane:
metabolism / Membrane Lipids: metabolism / Membrane Lipids:
chemistry / Lipid Bilayers: metabolism / Lipid Bilayers:
chemistry / Lipids: chemistry / Adsorption / Cholesterol
(NLM Chemicals) / Membrane Lipids (NLM Chemicals) / Lipid
Bilayers (NLM Chemicals) / Lipids (NLM Chemicals)},
cin = {AG Milovanovic (Berlin) / AG Milovanovic (Bonn)},
ddc = {500},
cid = {I:(DE-2719)1813002 / I:(DE-2719)1013043},
pnm = {351 - Brain Function (POF4-351)},
pid = {G:(DE-HGF)POF4-351},
typ = {PUB:(DE-HGF)16},
pubmed = {pmid:40113768},
pmc = {pmc:PMC11926106},
doi = {10.1038/s41467-025-57985-2},
url = {https://pub.dzne.de/record/277728},
}