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000277733 020__ $$a978-1-0716-4365-5 (print)
000277733 020__ $$a978-1-0716-4366-2 (electronic)
000277733 0247_ $$2doi$$a10.1007/978-1-0716-4366-2_12
000277733 0247_ $$2pmid$$apmid:40111604
000277733 0247_ $$2ISSN$$a1064-3745
000277733 0247_ $$2ISSN$$a1940-6029
000277733 037__ $$aDZNE-2025-00454
000277733 041__ $$aEnglish
000277733 082__ $$a570
000277733 1001_ $$aSeifert, Gerald$$b0
000277733 245__ $$aSuper-resolution Analysis of Astrocyte Morphology Using Expansion Microscopy.
000277733 260__ $$aNew York, NY$$bSpringer US$$c2025
000277733 29510 $$aAstrocytes / Di Benedetto, Barbara (Editor) ; New York, NY : Springer US, 2025, Chapter 12 ; ISSN: 1064-3745=1940-6029 ; ISBN: 978-1-0716-4365-5=978-1-0716-4366-2 ; doi:10.1007/978-1-0716-4366-2
000277733 300__ $$a165 - 179
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000277733 3367_ $$2BibTeX$$aINBOOK
000277733 3367_ $$2DataCite$$aOutput Types/Book chapter
000277733 3367_ $$0PUB:(DE-HGF)7$$2PUB:(DE-HGF)$$aContribution to a book$$bcontb$$mcontb$$s1743594354_11065
000277733 4900_ $$aMethods in Molecular Biology$$v2896
000277733 520__ $$aAnalyzing the structure of astrocytes, their specific morphological features, and their remodeling is important for understanding how this cell type fulfils its many functions. This is because cell shape determines the propagation of intracellular signals and their subcellular compartmentalization. At the same time, it determines which other cells in the neuropil an astrocyte is closely in contact with and can most effectively exchange signals with. One experimental challenge has been that the most abundant small astrocytic processes cannot be resolved with diffraction-limited microscopy. Typically, this obstacle was overcome by using electron microscopy, but the continuous development of super-resolution microscopy has produced many alternative techniques. One is expansion microscopy (ExM) [1]. ExM, in principle, expands the tissue while preserving the relative positioning of labels that mark structures of interest (e.g., fluorescent labels), which increases the effective spatial resolution of light microscopy without improving the spatial resolution of the microscope itself. The advantage of ExM is that it requires only a little more laboratory infrastructure than immunohistochemistry combined with confocal fluorescence microscopy. We have previously applied this universal technique to the analysis of the structure of astrocytes and of their fine processes and their perisynaptic arrangement. Here, we present a comprehensive protocol for visualizing and localizing astrocytes, synaptic structures, and synaptic and astrocytic proteins in fixed brain tissue.
000277733 536__ $$0G:(DE-HGF)POF4-351$$a351 - Brain Function (POF4-351)$$cPOF4-351$$fPOF IV$$x0
000277733 588__ $$aDataset connected to CrossRef Book Series, PubMed, , Journals: pub.dzne.de
000277733 650_7 $$2Other$$aAstrocyte morphology
000277733 650_7 $$2Other$$aExpansion microscopy
000277733 650_7 $$2Other$$aPerisynaptic astrocytic processes
000277733 650_7 $$2Other$$aSuper-resolution microscopy
000277733 650_2 $$2MeSH$$aAstrocytes: cytology
000277733 650_2 $$2MeSH$$aAstrocytes: ultrastructure
000277733 650_2 $$2MeSH$$aAstrocytes: metabolism
000277733 650_2 $$2MeSH$$aAnimals
000277733 650_2 $$2MeSH$$aMice
000277733 650_2 $$2MeSH$$aMicroscopy: methods
000277733 650_2 $$2MeSH$$aMicroscopy, Fluorescence: methods
000277733 650_2 $$2MeSH$$aImage Processing, Computer-Assisted: methods
000277733 7001_ $$aSommer, Erik$$b1
000277733 7001_ $$aPasslick, Stefan$$b2
000277733 7001_ $$0P:(DE-2719)2811625$$aHenneberger, Christian$$b3$$eLast author$$udzne
000277733 773__ $$a10.1007/978-1-0716-4366-2_12
000277733 8564_ $$uhttps://pub.dzne.de/record/277733/files/DZNE-2025-00454_Restricted.pdf
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000277733 9101_ $$0I:(DE-588)1065079516$$6P:(DE-2719)2811625$$aDeutsches Zentrum für Neurodegenerative Erkrankungen$$b3$$kDZNE
000277733 9131_ $$0G:(DE-HGF)POF4-351$$1G:(DE-HGF)POF4-350$$2G:(DE-HGF)POF4-300$$3G:(DE-HGF)POF4$$4G:(DE-HGF)POF$$aDE-HGF$$bGesundheit$$lNeurodegenerative Diseases$$vBrain Function$$x0
000277733 9141_ $$y2025
000277733 915__ $$0StatID:(DE-HGF)0200$$2StatID$$aDBCoverage$$bSCOPUS$$d2024-12-19
000277733 915__ $$0StatID:(DE-HGF)0300$$2StatID$$aDBCoverage$$bMedline$$d2024-12-19
000277733 9201_ $$0I:(DE-2719)1013029$$kAG Henneberger$$lSynaptic and Glial Plasticity$$x0
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000277733 980__ $$aI:(DE-2719)1013029
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