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@INBOOK{Seifert:277733,
      author       = {Seifert, Gerald and Sommer, Erik and Passlick, Stefan and
                      Henneberger, Christian},
      title        = {{S}uper-resolution {A}nalysis of {A}strocyte {M}orphology
                      {U}sing {E}xpansion {M}icroscopy.},
      volume       = {2896},
      address      = {New York, NY},
      publisher    = {Springer US},
      reportid     = {DZNE-2025-00454},
      isbn         = {978-1-0716-4365-5 (print)},
      series       = {Methods in Molecular Biology},
      pages        = {165 - 179},
      year         = {2025},
      comment      = {Astrocytes / Di Benedetto, Barbara (Editor) ; New York, NY
                      : Springer US, 2025, Chapter 12 ; ISSN: 1064-3745=1940-6029
                      ; ISBN: 978-1-0716-4365-5=978-1-0716-4366-2 ;
                      doi:10.1007/978-1-0716-4366-2},
      booktitle     = {Astrocytes / Di Benedetto, Barbara
                       (Editor) ; New York, NY : Springer US,
                       2025, Chapter 12 ; ISSN:
                       1064-3745=1940-6029 ; ISBN:
                       978-1-0716-4365-5=978-1-0716-4366-2 ;
                       doi:10.1007/978-1-0716-4366-2},
      abstract     = {Analyzing the structure of astrocytes, their specific
                      morphological features, and their remodeling is important
                      for understanding how this cell type fulfils its many
                      functions. This is because cell shape determines the
                      propagation of intracellular signals and their subcellular
                      compartmentalization. At the same time, it determines which
                      other cells in the neuropil an astrocyte is closely in
                      contact with and can most effectively exchange signals with.
                      One experimental challenge has been that the most abundant
                      small astrocytic processes cannot be resolved with
                      diffraction-limited microscopy. Typically, this obstacle was
                      overcome by using electron microscopy, but the continuous
                      development of super-resolution microscopy has produced many
                      alternative techniques. One is expansion microscopy (ExM)
                      [1]. ExM, in principle, expands the tissue while preserving
                      the relative positioning of labels that mark structures of
                      interest (e.g., fluorescent labels), which increases the
                      effective spatial resolution of light microscopy without
                      improving the spatial resolution of the microscope itself.
                      The advantage of ExM is that it requires only a little more
                      laboratory infrastructure than immunohistochemistry combined
                      with confocal fluorescence microscopy. We have previously
                      applied this universal technique to the analysis of the
                      structure of astrocytes and of their fine processes and
                      their perisynaptic arrangement. Here, we present a
                      comprehensive protocol for visualizing and localizing
                      astrocytes, synaptic structures, and synaptic and astrocytic
                      proteins in fixed brain tissue.},
      keywords     = {Astrocytes: cytology / Astrocytes: ultrastructure /
                      Astrocytes: metabolism / Animals / Mice / Microscopy:
                      methods / Microscopy, Fluorescence: methods / Image
                      Processing, Computer-Assisted: methods / Astrocyte
                      morphology (Other) / Expansion microscopy (Other) /
                      Perisynaptic astrocytic processes (Other) / Super-resolution
                      microscopy (Other)},
      cin          = {AG Henneberger},
      ddc          = {570},
      cid          = {I:(DE-2719)1013029},
      pnm          = {351 - Brain Function (POF4-351)},
      pid          = {G:(DE-HGF)POF4-351},
      typ          = {PUB:(DE-HGF)7},
      pubmed       = {pmid:40111604},
      doi          = {10.1007/978-1-0716-4366-2_12},
      url          = {https://pub.dzne.de/record/277733},
}