TY  - JOUR
AU  - Fischer, Anna-Lisa
AU  - Schmitz, Matthias
AU  - Thom, Tobias
AU  - Zafar, Saima
AU  - Younas, Neelam
AU  - da Silva Correia, Susana
AU  - Silva-Correia, Susana
AU  - Canaslan, Sezgi
AU  - Zerr, Inga
TI  - Alpha-Synuclein Demonstrates Varying Binding Affinities With Different Tau Isoforms.
JO  - Journal of neurochemistry
VL  - 169
IS  - 4
SN  - 0022-3042
CY  - Oxford
PB  - Wiley-Blackwell
M1  - DZNE-2025-00479
SP  - e70053
PY  - 2025
AB  - The hallmark of various neurodegenerative diseases is the accumulation and aggregation of amyloidogenic proteins, such as amyloid-beta (Aβ) and tau in Alzheimer's disease (AD) and alpha-synuclein (aSyn) in synucleinopathies. Many neurodegenerative diseases express mixed pathology. For instance, Lewy bodies are reported in tauopathies and neurofibrillary tau-tangles are detected in synucleinopathies, suggesting a potential co-existence or crosstalk of misfolded aSyn and tau. In the present study, we investigated the binding characteristics of monomeric aSyn with different tau isoforms by using surface plasmon resonance (SPR) spectroscopy allowing monitoring direct protein-protein interactions and their potential co-localization using SH-SY5Y cells. The calculation of the binding parameters (association and dissociation rate constants) indicated the strongest binding affinity between aSyn and tau isoform 1N3R followed by tau 2N3R and tau 2N4R. Co-localization studies in SH-SY5Y cells, treated with aSyn and all six tau isoforms revealed an intracellular co-localization of aSyn with different isoforms of tau. Subcellular fractionation confirmed the cellular uptake and colocalization of tau and aSyn in the same compartment, showing their expression in membrane, nuclear, and cytoskeletal fractions. Understanding the intricate interplay between aSyn and tau is crucial for unraveling the pathophysiology of PD, AD, and related neurodegenerative disorders, ultimately paving the way for the development of effective treatments targeting this interaction. In conclusion, our data indicate that aSyn and tau are direct interaction partners with varying binding affinities depending on the tau isoform. This interaction may be significant for understanding the pathophysiology of dementia with mixed pathologies.
KW  - Humans
KW  - tau Proteins: metabolism
KW  - alpha-Synuclein: metabolism
KW  - Protein Isoforms: metabolism
KW  - Protein Binding: physiology
KW  - Cell Line, Tumor
KW  - Surface Plasmon Resonance
KW  - Alzheimer's disease (Other)
KW  - Parkinson's disease (Other)
KW  - alpha‐synuclein (Other)
KW  - interaction (Other)
KW  - synucleinopathies (Other)
KW  - tau (Other)
KW  - tauopathies (Other)
KW  - tau Proteins (NLM Chemicals)
KW  - alpha-Synuclein (NLM Chemicals)
KW  - Protein Isoforms (NLM Chemicals)
LB  - PUB:(DE-HGF)16
C6  - pmid:40165586
DO  - DOI:10.1111/jnc.70053
UR  - https://pub.dzne.de/record/277807
ER  -