TY - JOUR AU - Kaurani, Lalit AU - Pradhan, Ranjit AU - Schröder, Sophie AU - Burkhardt, Susanne AU - Schuetz, Anna-Lena AU - Krüger, Dennis M AU - Pena, Tonatiuh AU - Heutink, Peter AU - Sananbenesi, Farahnaz AU - Fischer, Andre TI - A role for astrocytic miR-129-5p in frontotemporal dementia. JO - Translational Psychiatry VL - 15 IS - 1 SN - 2158-3188 CY - London PB - Nature Publishing Group M1 - DZNE-2025-00518 SP - 142 PY - 2025 AB - Frontotemporal dementia is a debilitating neurodegenerative disorder characterized by frontal and temporal lobe degeneration, resulting in behavioral changes, language difficulties, and cognitive decline. In this study, smallRNA sequencing was conducted on postmortem brain tissues obtained from the frontal and temporal of FTD patients with GRN, MAPT, or C9ORF72 mutations. Our analysis identified miR-129-5p as consistently deregulated across all analyzed mutation conditions and brain regions. Functional investigations in in-vitro models revealed a novel role of miR-129-5p in astrocytes, where its loss led to neuroinflammation and impaired neuronal support functions, including reduced glutamate uptake. Depletion of miR-129-5p in astrocytes also resulted in the loss of neuronal spines and altered neuronal network activity in a cell culture system. These findings highlight miR-129-5p as a potential therapeutic target in neurodegenerative diseases and also sheds light on the role of astrocytes in Frontotemporal dementia pathogenesis. KW - Humans KW - Frontotemporal Dementia: genetics KW - Frontotemporal Dementia: metabolism KW - Frontotemporal Dementia: pathology KW - Astrocytes: metabolism KW - MicroRNAs: genetics KW - MicroRNAs: metabolism KW - tau Proteins: genetics KW - Male KW - Female KW - C9orf72 Protein: genetics KW - Progranulins: genetics KW - Middle Aged KW - Mutation KW - Aged KW - Brain: metabolism KW - Glutamic Acid: metabolism KW - MicroRNAs (NLM Chemicals) KW - tau Proteins (NLM Chemicals) KW - Mirn129 microRNA, human (NLM Chemicals) KW - MAPT protein, human (NLM Chemicals) KW - C9orf72 Protein (NLM Chemicals) KW - Progranulins (NLM Chemicals) KW - GRN protein, human (NLM Chemicals) KW - C9orf72 protein, human (NLM Chemicals) KW - Glutamic Acid (NLM Chemicals) LB - PUB:(DE-HGF)16 C6 - pmid:40216778 C2 - pmc:PMC11992244 DO - DOI:10.1038/s41398-025-03338-y UR - https://pub.dzne.de/record/277991 ER -