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@ARTICLE{Breunig:277995,
      author       = {Breunig, Markus and Hohwieler, Meike and Haderspeck, Jasmin
                      and Zweydorf, Felix and Hauff, Natalie and Pasquini,
                      Lino-Pascal and Wiegreffe, Christoph and Zimmer, Eleni and
                      Mulaw, Medhanie A and Julier, Cécile and Simon, Eric and
                      Gloeckner, Christian Johannes and Liebau, Stefan and Kleger,
                      Alexander},
      title        = {{PPDPF} is not a key regulator of human pancreas
                      development.},
      journal      = {PLoS Genetics},
      volume       = {21},
      number       = {4},
      issn         = {1553-7390},
      address      = {San Francisco, Calif.},
      publisher    = {Public Library of Science},
      reportid     = {DZNE-2025-00522},
      pages        = {e1011657},
      year         = {2025},
      abstract     = {Given their capability to differentiate into each cell type
                      of the human body, human pluripotent stem cells (hPSCs)
                      provide a unique platform for developmental studies. In the
                      current study, we employed this cell system to understand
                      the role of pancreatic progenitor differentiation and
                      proliferation factor (PPDPF), a protein that has been little
                      explored so far. While the zebrafish orthologue exdpf is
                      essential for exocrine pancreas specification, its
                      importance for mammalian and human development has not been
                      studied yet. We implemented a four times CRISPR/Cas9 nicking
                      approach to knockout PPDPF in human embryonic stem cells
                      (hESCs) and differentiated PPDPFKO/KO and PPDPFWT/WT cells
                      towards the pancreatic lineage. In contrast to data obtained
                      from zebrafish, a very modest effect of the knockout was
                      observed in the development of pancreatic progenitors in
                      vitro, not affecting lineage specification upon orthotopic
                      transplantation in vivo. The modest effect is in line with
                      the finding that genetic variants near PPDPF are associated
                      with random glucose levels in humans, but not with type 2
                      diabetes risk, supporting that dysregulation of this gene
                      may only result in minor alterations of glycaemic balance in
                      humans. In addition, PPDPF is less organ- and cell type
                      specifically expressed in higher vertebrates and its so far
                      reported functions appear highly context-dependent.},
      cin          = {AG Gloeckner},
      ddc          = {610},
      cid          = {I:(DE-2719)1210007},
      pnm          = {352 - Disease Mechanisms (POF4-352)},
      pid          = {G:(DE-HGF)POF4-352},
      typ          = {PUB:(DE-HGF)16},
      pubmed       = {pmid:40193385},
      doi          = {10.1371/journal.pgen.1011657},
      url          = {https://pub.dzne.de/record/277995},
}