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000278034 041__ $$aEnglish
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000278034 1001_ $$0P:(DE-HGF)0$$aWang, Huan$$b0
000278034 245__ $$aLive-cell quantification reveals viscoelastic regulation of synapsin condensates by α-synuclein.
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000278034 520__ $$aSynapsin and α-synuclein represent a growing list of condensate-forming proteins where the material states of condensates are directly linked to cellular functions (e.g., neurotransmission) and pathology (e.g., neurodegeneration). However, quantifying condensate material properties in living systems has been a substantial challenge. Here, we develop micropipette aspiration and whole-cell patch-clamp (MAPAC), a platform that allows direct material quantification of condensates in live cells. We find 10,000-fold variations in the viscoelasticity of synapsin condensates, regulated by the partitioning of α-synuclein, a marker for synucleinopathies. Through in vitro reconstitutions, we identify multiple molecular factors that distinctly regulate the viscosity, interfacial tension, and maturation of synapsin condensates, confirming the cellular roles of α-synuclein. Overall, our study provides unprecedented quantitative insights into the material properties of neuronal condensates and reveals a crucial role of α-synuclein in regulating condensate viscoelasticity. Furthermore, we envision MAPAC applicable to study a broad range of condensates in vivo.
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000278034 650_7 $$2NLM Chemicals$$aalpha-Synuclein
000278034 650_7 $$2NLM Chemicals$$aSynapsins
000278034 650_2 $$2MeSH$$aalpha-Synuclein: metabolism
000278034 650_2 $$2MeSH$$aalpha-Synuclein: chemistry
000278034 650_2 $$2MeSH$$aViscosity
000278034 650_2 $$2MeSH$$aHumans
000278034 650_2 $$2MeSH$$aSynapsins: metabolism
000278034 650_2 $$2MeSH$$aSynapsins: chemistry
000278034 650_2 $$2MeSH$$aElasticity
000278034 650_2 $$2MeSH$$aAnimals
000278034 650_2 $$2MeSH$$aBiomolecular Condensates: metabolism
000278034 650_2 $$2MeSH$$aBiomolecular Condensates: chemistry
000278034 650_2 $$2MeSH$$aPatch-Clamp Techniques
000278034 7001_ $$0P:(DE-2719)9000582$$aHoffmann, Christian$$b1
000278034 7001_ $$0P:(DE-2719)9002092$$aTromm, Johannes V$$b2$$udzne
000278034 7001_ $$00000-0001-6408-8273$$aSu, Xiao$$b3
000278034 7001_ $$00009-0002-9175-4440$$aElliott, Jordan$$b4
000278034 7001_ $$0P:(DE-2719)9001572$$aWang, Han$$b5$$udzne
000278034 7001_ $$00000-0003-3932-2552$$aDeng, Mengying$$b6
000278034 7001_ $$00000-0002-0859-3854$$aMcClenaghan, Conor$$b7
000278034 7001_ $$aBaum, Jean$$b8
000278034 7001_ $$00000-0002-6183-1233$$aPang, Zhiping P$$b9
000278034 7001_ $$0P:(DE-2719)9000670$$aMilovanovic, Dragomir$$b10
000278034 7001_ $$00000-0002-5214-1871$$aShi, Zheng$$b11
000278034 773__ $$0PERI:(DE-600)2810933-8$$a10.1126/sciadv.ads7627$$gVol. 11, no. 16, p. eads7627$$n16$$peads7627$$tScience advances$$v11$$x2375-2548$$y2025
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