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@ARTICLE{Wang:278034,
      author       = {Wang, Huan and Hoffmann, Christian and Tromm, Johannes V
                      and Su, Xiao and Elliott, Jordan and Wang, Han and Deng,
                      Mengying and McClenaghan, Conor and Baum, Jean and Pang,
                      Zhiping P and Milovanovic, Dragomir and Shi, Zheng},
      title        = {{L}ive-cell quantification reveals viscoelastic regulation
                      of synapsin condensates by α-synuclein.},
      journal      = {Science advances},
      volume       = {11},
      number       = {16},
      issn         = {2375-2548},
      address      = {Washington, DC [u.a.]},
      publisher    = {Assoc.},
      reportid     = {DZNE-2025-00540},
      pages        = {eads7627},
      year         = {2025},
      abstract     = {Synapsin and α-synuclein represent a growing list of
                      condensate-forming proteins where the material states of
                      condensates are directly linked to cellular functions (e.g.,
                      neurotransmission) and pathology (e.g., neurodegeneration).
                      However, quantifying condensate material properties in
                      living systems has been a substantial challenge. Here, we
                      develop micropipette aspiration and whole-cell patch-clamp
                      (MAPAC), a platform that allows direct material
                      quantification of condensates in live cells. We find
                      10,000-fold variations in the viscoelasticity of synapsin
                      condensates, regulated by the partitioning of α-synuclein,
                      a marker for synucleinopathies. Through in vitro
                      reconstitutions, we identify multiple molecular factors that
                      distinctly regulate the viscosity, interfacial tension, and
                      maturation of synapsin condensates, confirming the cellular
                      roles of α-synuclein. Overall, our study provides
                      unprecedented quantitative insights into the material
                      properties of neuronal condensates and reveals a crucial
                      role of α-synuclein in regulating condensate
                      viscoelasticity. Furthermore, we envision MAPAC applicable
                      to study a broad range of condensates in vivo.},
      keywords     = {alpha-Synuclein: metabolism / alpha-Synuclein: chemistry /
                      Viscosity / Humans / Synapsins: metabolism / Synapsins:
                      chemistry / Elasticity / Animals / Biomolecular Condensates:
                      metabolism / Biomolecular Condensates: chemistry /
                      Patch-Clamp Techniques / alpha-Synuclein (NLM Chemicals) /
                      Synapsins (NLM Chemicals)},
      cin          = {AG Milovanovic (Berlin) / AG Milovanovic (Bonn)},
      ddc          = {500},
      cid          = {I:(DE-2719)1813002 / I:(DE-2719)1013043},
      pnm          = {351 - Brain Function (POF4-351)},
      pid          = {G:(DE-HGF)POF4-351},
      typ          = {PUB:(DE-HGF)16},
      pubmed       = {pmid:40249817},
      pmc          = {pmc:PMC12007584},
      doi          = {10.1126/sciadv.ads7627},
      url          = {https://pub.dzne.de/record/278034},
}