000278658 001__ 278658
000278658 005__ 20250601001327.0
000278658 0247_ $$2doi$$a10.1128/mbio.03314-24
000278658 0247_ $$2pmid$$apmid:40167317
000278658 0247_ $$2pmc$$apmc:PMC12077180
000278658 0247_ $$2ISSN$$a2161-2129
000278658 0247_ $$2ISSN$$a2150-7511
000278658 0247_ $$2altmetric$$aaltmetric:177345968
000278658 037__ $$aDZNE-2025-00614
000278658 041__ $$aEnglish
000278658 082__ $$a570
000278658 1001_ $$aChen, Yuexuan$$b0
000278658 245__ $$aRAB5 is a host dependency factor for the generation of SARS-CoV-2 replication organelles.
000278658 260__ $$aWashington, DC$$bAmerican Society for Microbiology$$c2025
000278658 3367_ $$2DRIVER$$aarticle
000278658 3367_ $$2DataCite$$aOutput Types/Journal article
000278658 3367_ $$0PUB:(DE-HGF)16$$2PUB:(DE-HGF)$$aJournal Article$$bjournal$$mjournal$$s1748429112_1773
000278658 3367_ $$2BibTeX$$aARTICLE
000278658 3367_ $$2ORCID$$aJOURNAL_ARTICLE
000278658 3367_ $$00$$2EndNote$$aJournal Article
000278658 520__ $$aSevere acute respiratory syndrome coronavirus 2 (SARS-CoV-2) remains a threat due to the emergence of variants with increased transmissibility and enhanced escape from immune responses. Like other coronaviruses before, SARS-CoV-2 likely emerged after its transmission from bats. The successful propagation of SARS-CoV-2 in humans might have been facilitated by usurping evolutionarily conserved cellular factors to execute crucial steps in its life cycle, such as the generation of replication organelles-membrane structures where coronaviruses assemble their replication-transcription complex. In this study, we found that RAB5, which is highly conserved across mammals, is a critical host dependency factor for the replication of the SARS-CoV-2 genome. Our results also suggest that SARS-CoV-2 uses RAB5+ membranes to build replication organelles with the aid of COPB1, a component of the COP-I complex, and that the virus protein NSP6 participates in this process. Hence, targeting NSP6 represents a promising approach to interfere with SARS-CoV-2 RNA synthesis and halt its propagation.IMPORTANCEIn this study, we sought to identify the host dependency factors that severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) uses for the generation of replication organelles: cellular membranous structures that SARS-CoV-2 builds in order to support the replication and transcription of its genome. We uncovered that RAB5 is an important dependency factor for SARS-CoV-2 replication and the generation of replication organelles, and that the viral protein NSP6 participates in this process. Hence, NSP6 represents a promising target to halt SARS-CoV-2 replication.
000278658 536__ $$0G:(DE-HGF)POF4-351$$a351 - Brain Function (POF4-351)$$cPOF4-351$$fPOF IV$$x0
000278658 588__ $$aDataset connected to CrossRef, PubMed, , Journals: pub.dzne.de
000278658 650_7 $$2Other$$aCOP-I
000278658 650_7 $$2Other$$aCOPB1
000278658 650_7 $$2Other$$aNSP6
000278658 650_7 $$2Other$$aRAB5
000278658 650_7 $$2Other$$aSARS-CoV-2
000278658 650_7 $$2Other$$areplication organelle
000278658 650_7 $$0EC 3.6.5.2$$2NLM Chemicals$$arab5 GTP-Binding Proteins
000278658 650_7 $$0EC 3.6.1.-$$2NLM Chemicals$$aRAB5C protein, human
000278658 650_2 $$2MeSH$$aHumans
000278658 650_2 $$2MeSH$$arab5 GTP-Binding Proteins: metabolism
000278658 650_2 $$2MeSH$$arab5 GTP-Binding Proteins: genetics
000278658 650_2 $$2MeSH$$aVirus Replication
000278658 650_2 $$2MeSH$$aSARS-CoV-2: physiology
000278658 650_2 $$2MeSH$$aSARS-CoV-2: genetics
000278658 650_2 $$2MeSH$$aAnimals
000278658 650_2 $$2MeSH$$aCOVID-19: virology
000278658 650_2 $$2MeSH$$aHost-Pathogen Interactions
000278658 650_2 $$2MeSH$$aVero Cells
000278658 650_2 $$2MeSH$$aChlorocebus aethiops
000278658 650_2 $$2MeSH$$aViral Replication Compartments: metabolism
000278658 7001_ $$0P:(DE-2719)9003510$$aKlute, Susanne$$b1$$udzne
000278658 7001_ $$0P:(DE-2719)9003481$$aSparrer, Konstantin Maria Johannes$$b2$$udzne
000278658 7001_ $$00000-0002-1923-6414$$aSerra-Moreno, Ruth$$b3
000278658 773__ $$0PERI:(DE-600)2557172-2$$a10.1128/mbio.03314-24$$gVol. 16, no. 5, p. e03314-24$$n5$$pe0331424$$tmBio$$v16$$x2161-2129$$y2025
000278658 8564_ $$uhttps://pub.dzne.de/record/278658/files/DZNE-2025-00614%20SUP.pdf
000278658 8564_ $$uhttps://pub.dzne.de/record/278658/files/DZNE-2025-00614.pdf$$yOpenAccess
000278658 8564_ $$uhttps://pub.dzne.de/record/278658/files/DZNE-2025-00614%20SUP.pdf?subformat=pdfa$$xpdfa
000278658 8564_ $$uhttps://pub.dzne.de/record/278658/files/DZNE-2025-00614.pdf?subformat=pdfa$$xpdfa$$yOpenAccess
000278658 909CO $$ooai:pub.dzne.de:278658$$pdnbdelivery$$pdriver$$pVDB$$popen_access$$popenaire
000278658 9101_ $$0I:(DE-HGF)0$$6P:(DE-2719)9003510$$aExternal Institute$$b1$$kExtern
000278658 9101_ $$0I:(DE-588)1065079516$$6P:(DE-2719)9003481$$aDeutsches Zentrum für Neurodegenerative Erkrankungen$$b2$$kDZNE
000278658 9131_ $$0G:(DE-HGF)POF4-351$$1G:(DE-HGF)POF4-350$$2G:(DE-HGF)POF4-300$$3G:(DE-HGF)POF4$$4G:(DE-HGF)POF$$aDE-HGF$$bGesundheit$$lNeurodegenerative Diseases$$vBrain Function$$x0
000278658 9141_ $$y2025
000278658 915__ $$0StatID:(DE-HGF)0200$$2StatID$$aDBCoverage$$bSCOPUS$$d2024-12-18
000278658 915__ $$0StatID:(DE-HGF)0160$$2StatID$$aDBCoverage$$bEssential Science Indicators$$d2024-12-18
000278658 915__ $$0StatID:(DE-HGF)1050$$2StatID$$aDBCoverage$$bBIOSIS Previews$$d2024-12-18
000278658 915__ $$0StatID:(DE-HGF)1190$$2StatID$$aDBCoverage$$bBiological Abstracts$$d2024-12-18
000278658 915__ $$0LIC:(DE-HGF)CCBY4$$2HGFVOC$$aCreative Commons Attribution CC BY 4.0
000278658 915__ $$0StatID:(DE-HGF)0100$$2StatID$$aJCR$$bMBIO : 2022$$d2024-12-18
000278658 915__ $$0StatID:(DE-HGF)0501$$2StatID$$aDBCoverage$$bDOAJ Seal$$d2023-03-14T07:11:37Z
000278658 915__ $$0StatID:(DE-HGF)0500$$2StatID$$aDBCoverage$$bDOAJ$$d2023-03-14T07:11:37Z
000278658 915__ $$0StatID:(DE-HGF)0113$$2StatID$$aWoS$$bScience Citation Index Expanded$$d2024-12-18
000278658 915__ $$0StatID:(DE-HGF)0700$$2StatID$$aFees$$d2024-12-18
000278658 915__ $$0StatID:(DE-HGF)0150$$2StatID$$aDBCoverage$$bWeb of Science Core Collection$$d2024-12-18
000278658 915__ $$0StatID:(DE-HGF)0510$$2StatID$$aOpenAccess
000278658 915__ $$0StatID:(DE-HGF)0030$$2StatID$$aPeer Review$$bDOAJ : Anonymous peer review$$d2023-03-14T07:11:37Z
000278658 915__ $$0StatID:(DE-HGF)0561$$2StatID$$aArticle Processing Charges$$d2024-12-18
000278658 915__ $$0StatID:(DE-HGF)9905$$2StatID$$aIF >= 5$$bMBIO : 2022$$d2024-12-18
000278658 915__ $$0StatID:(DE-HGF)0300$$2StatID$$aDBCoverage$$bMedline$$d2024-12-18
000278658 915__ $$0StatID:(DE-HGF)0199$$2StatID$$aDBCoverage$$bClarivate Analytics Master Journal List$$d2024-12-18
000278658 9201_ $$0I:(DE-2719)1910003$$kAG Sparrer$$lNeurovirology and Neuroinflammation$$x0
000278658 980__ $$ajournal
000278658 980__ $$aVDB
000278658 980__ $$aUNRESTRICTED
000278658 980__ $$aI:(DE-2719)1910003
000278658 9801_ $$aFullTexts