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@ARTICLE{Emery:278929,
author = {Emery, Brett Addison and Hu, Xin and Klütsch, Diana and
Khanzada, Shahrukh and Larsson, Ludvig and Dumitru, Ionut
and Frisén, Jonas and Lundeberg, Joakim and Kempermann,
Gerd and Amin, Hayder},
title = {{MEA}-seq{X}: {H}igh-{R}esolution {P}rofiling of
{L}arge-{S}cale {E}lectrophysiological and {T}ranscriptional
{N}etwork {D}ynamics.},
journal = {Advanced science},
volume = {12},
number = {20},
issn = {2198-3844},
address = {Weinheim},
publisher = {Wiley-VCH},
reportid = {DZNE-2025-00655},
pages = {2412373},
year = {2025},
abstract = {Concepts of brain function imply congruence and mutual
causal influence between molecular events and neuronal
activity. Decoding entangled information from concurrent
molecular and electrophysiological network events demands
innovative methodology bridging scales and modalities. The
MEA-seqX platform, integrating high-density microelectrode
arrays, spatial transcriptomics, optical imaging, and
advanced computational strategies, enables the simultaneous
recording and analysis of molecular and electrical network
activities at mesoscale spatial resolution. Applied to a
mouse hippocampal model of experience-dependent plasticity,
MEA-seqX unveils massively enhanced nested dynamics between
transcription and function. Graph-theoretic analysis reveals
an increase in densely connected bimodal hubs, marking the
first observation of coordinated hippocampal circuitry
dynamics at molecular and functional levels. This platform
also identifies different cell types based on their distinct
bimodal profiles. Machine-learning algorithms accurately
predict network-wide electrophysiological activity features
from spatial gene expression, demonstrating a previously
inaccessible convergence across modalities, time, and
scales.},
keywords = {Animals / Mice / Hippocampus: physiology / Hippocampus:
metabolism / Gene Regulatory Networks: genetics /
Electrophysiological Phenomena: physiology / Gene Expression
Profiling: methods / Neuronal Plasticity: physiology /
Neurons: physiology / Machine Learning / AI
machine‐learning (Other) / connectome (Other) /
experience‐dependent plasticity (Other) / large‐scale
neural recordings (Other) / predictive modeling (Other) /
spatial transcriptomics (Other) / spatiotemporal dynamics
(Other)},
cin = {AG Amin / AG Kempermann},
ddc = {624},
cid = {I:(DE-2719)1710010 / I:(DE-2719)1710001},
pnm = {351 - Brain Function (POF4-351) / 352 - Disease Mechanisms
(POF4-352)},
pid = {G:(DE-HGF)POF4-351 / G:(DE-HGF)POF4-352},
typ = {PUB:(DE-HGF)16},
pubmed = {pmid:40304297},
pmc = {pmc:PMC12120740},
doi = {10.1002/advs.202412373},
url = {https://pub.dzne.de/record/278929},
}