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000279042 1001_ $$aLourbopoulos, Athanasios$$b0
000279042 245__ $$aAn Improved Method for Sampling and Quantitative Protein Analytics of Cerebrospinal Fluid of Individual Mice.
000279042 260__ $$aBethesda, Md.$$bThe American Society for Biochemistry and Molecular Biology$$c2025
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000279042 520__ $$aThe mouse is the species most commonly used in preclinical research, but protein analytics of murine cerebrospinal fluid (CSF) remains challenging because of low sample volumes (often <10 μl) and frequent contaminations with blood. We developed an improved CSF sampling method that allows routine collection of larger volumes (20-30 μl) of pure CSF from individual mice, enabling multiple protein analytical assays from a single sample. Based on cell counts and hemoglobin ELISAs, we provide an easy quality control workflow for obtaining cell- and blood-free murine CSF. Through mass spectrometry-based proteomics using an absolutely quantified external standard, we estimated concentrations for hundreds of mouse CSF proteins. While repeated CSF sampling from the same mouse was possible, it induced CSF proteome changes. Applying the improved method, we found that the mouse CSF proteome remains largely stable over time in wild-type mice, but that amyloid pathology in the 5xFAD mouse model of Alzheimer's disease massively changes the CSF proteome. Neurofilament light chain and TREM2, markers of neurodegeneration and activated microglia, respectively, were strongly upregulated and validated using immunoassays. In conclusion, our refined murine CSF collection method overcomes previous limitations, allowing multiple quantitative protein analyses for applications in biomedicine.
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000279042 650_7 $$2Other$$aCSF collection
000279042 650_7 $$2Other$$aaging
000279042 650_7 $$2Other$$acerebrospinal fluid
000279042 650_7 $$2Other$$amethod
000279042 650_7 $$2Other$$amouse
000279042 650_7 $$2Other$$aneurodegeneration
000279042 650_7 $$2Other$$aquantitative proteomics
000279042 650_7 $$2Other$$atrauma
000279042 650_7 $$2NLM Chemicals$$aCerebrospinal Fluid Proteins
000279042 650_7 $$2NLM Chemicals$$aProteome
000279042 650_7 $$2NLM Chemicals$$aBiomarkers
000279042 650_7 $$2NLM Chemicals$$aNeurofilament Proteins
000279042 650_7 $$2NLM Chemicals$$aneurofilament protein L
000279042 650_2 $$2MeSH$$aAnimals
000279042 650_2 $$2MeSH$$aMice
000279042 650_2 $$2MeSH$$aProteomics: methods
000279042 650_2 $$2MeSH$$aAlzheimer Disease: cerebrospinal fluid
000279042 650_2 $$2MeSH$$aCerebrospinal Fluid Proteins
000279042 650_2 $$2MeSH$$aProteome: metabolism
000279042 650_2 $$2MeSH$$aMice, Inbred C57BL
000279042 650_2 $$2MeSH$$aDisease Models, Animal
000279042 650_2 $$2MeSH$$aBiomarkers: cerebrospinal fluid
000279042 650_2 $$2MeSH$$aMice, Transgenic
000279042 650_2 $$2MeSH$$aNeurofilament Proteins: cerebrospinal fluid
000279042 7001_ $$0P:(DE-2719)2810938$$aMüller, Stephan A$$b1$$udzne
000279042 7001_ $$0P:(DE-2719)2813355$$aJocher, Georg$$b2$$udzne
000279042 7001_ $$aWick, Manfred$$b3
000279042 7001_ $$aPlesnila, Nikolaus$$b4
000279042 7001_ $$0P:(DE-2719)2181459$$aLichtenthaler, Stefan F$$b5$$eLast author$$udzne
000279042 773__ $$0PERI:(DE-600)2071375-7$$a10.1016/j.mcpro.2025.100958$$gVol. 24, no. 5, p. 100958 -$$n5$$p100958$$tMolecular & cellular proteomics$$v24$$x1535-9476$$y2025
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