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@ARTICLE{Lourbopoulos:279042,
      author       = {Lourbopoulos, Athanasios and Müller, Stephan A and Jocher,
                      Georg and Wick, Manfred and Plesnila, Nikolaus and
                      Lichtenthaler, Stefan F},
      title        = {{A}n {I}mproved {M}ethod for {S}ampling and {Q}uantitative
                      {P}rotein {A}nalytics of {C}erebrospinal {F}luid of
                      {I}ndividual {M}ice.},
      journal      = {Molecular $\&$ cellular proteomics},
      volume       = {24},
      number       = {5},
      issn         = {1535-9476},
      address      = {Bethesda, Md.},
      publisher    = {The American Society for Biochemistry and Molecular
                      Biology},
      reportid     = {DZNE-2025-00672},
      pages        = {100958},
      year         = {2025},
      abstract     = {The mouse is the species most commonly used in preclinical
                      research, but protein analytics of murine cerebrospinal
                      fluid (CSF) remains challenging because of low sample
                      volumes (often <10 μl) and frequent contaminations with
                      blood. We developed an improved CSF sampling method that
                      allows routine collection of larger volumes (20-30 μl) of
                      pure CSF from individual mice, enabling multiple protein
                      analytical assays from a single sample. Based on cell counts
                      and hemoglobin ELISAs, we provide an easy quality control
                      workflow for obtaining cell- and blood-free murine CSF.
                      Through mass spectrometry-based proteomics using an
                      absolutely quantified external standard, we estimated
                      concentrations for hundreds of mouse CSF proteins. While
                      repeated CSF sampling from the same mouse was possible, it
                      induced CSF proteome changes. Applying the improved method,
                      we found that the mouse CSF proteome remains largely stable
                      over time in wild-type mice, but that amyloid pathology in
                      the 5xFAD mouse model of Alzheimer's disease massively
                      changes the CSF proteome. Neurofilament light chain and
                      TREM2, markers of neurodegeneration and activated microglia,
                      respectively, were strongly upregulated and validated using
                      immunoassays. In conclusion, our refined murine CSF
                      collection method overcomes previous limitations, allowing
                      multiple quantitative protein analyses for applications in
                      biomedicine.},
      keywords     = {Animals / Mice / Proteomics: methods / Alzheimer Disease:
                      cerebrospinal fluid / Cerebrospinal Fluid Proteins /
                      Proteome: metabolism / Mice, Inbred C57BL / Disease Models,
                      Animal / Biomarkers: cerebrospinal fluid / Mice, Transgenic
                      / Neurofilament Proteins: cerebrospinal fluid / CSF
                      collection (Other) / aging (Other) / cerebrospinal fluid
                      (Other) / method (Other) / mouse (Other) / neurodegeneration
                      (Other) / quantitative proteomics (Other) / trauma (Other) /
                      Cerebrospinal Fluid Proteins (NLM Chemicals) / Proteome (NLM
                      Chemicals) / Biomarkers (NLM Chemicals) / Neurofilament
                      Proteins (NLM Chemicals) / neurofilament protein L (NLM
                      Chemicals)},
      cin          = {AG Lichtenthaler},
      ddc          = {610},
      cid          = {I:(DE-2719)1110006},
      pnm          = {352 - Disease Mechanisms (POF4-352)},
      pid          = {G:(DE-HGF)POF4-352},
      typ          = {PUB:(DE-HGF)16},
      pubmed       = {pmid:40157722},
      pmc          = {pmc:PMC12090247},
      doi          = {10.1016/j.mcpro.2025.100958},
      url          = {https://pub.dzne.de/record/279042},
}