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@ARTICLE{Gavoci:279358,
      author       = {Gavoci, Antoneta and Zhiti, Anxhela and Rusková, Michaela
                      and Magiera, Maria M and Wang, Mengzhe and Ziegler, Karin A
                      and Hausrat, Torben J and Ugwuja, Anselm I and Chakraborty,
                      Shreyangi and Engelhardt, Stefan and Kneussel, Matthias and
                      Balastik, Martin and Janke, Carsten and Misgeld, Thomas and
                      Brill, Monika S},
      title        = {{P}olyglutamylation of microtubules drives neuronal
                      remodeling.},
      journal      = {Nature Communications},
      volume       = {16},
      number       = {1},
      issn         = {2041-1723},
      address      = {[London]},
      publisher    = {Springer Nature},
      reportid     = {DZNE-2025-00735},
      pages        = {5384},
      year         = {2025},
      abstract     = {Developmental remodeling shapes neural circuits via
                      activity-dependent pruning of synapses and axons. Regulation
                      of the cytoskeleton is critical for this process, as
                      microtubule loss via enzymatic severing is an early step of
                      pruning across many circuits and species. However, how
                      microtubule-severing enzymes, such as spastin, are activated
                      in specific neuronal compartments remains unknown. Here, we
                      reveal that polyglutamylation, a post-translational tubulin
                      modification enriched in neurons, plays an instructive role
                      in developmental remodeling by tagging microtubules for
                      severing. Motor neuron-specific gene deletion of enzymes
                      that add or remove tubulin polyglutamylation-TTLL
                      glutamylases vs. CCP deglutamylases-accelerates or delays
                      neuromuscular synapse remodeling in a
                      neurotransmission-dependent manner. This mechanism is not
                      specific to peripheral synapses but also operates in central
                      circuits, e.g., the hippocampus. Thus, tubulin
                      polyglutamylation acts as a cytoskeletal rheostat of
                      remodeling that shapes neuronal morphology and
                      connectivity.},
      keywords     = {Microtubules: metabolism / Animals / Tubulin: metabolism /
                      Peptide Synthases: metabolism / Peptide Synthases: genetics
                      / Motor Neurons: metabolism / Hippocampus: metabolism /
                      Hippocampus: cytology / Neuronal Plasticity: physiology /
                      Synapses: metabolism / Synaptic Transmission / Neurons:
                      metabolism / Polyglutamic Acid: metabolism / Neuromuscular
                      Junction: metabolism / Protein Processing,
                      Post-Translational / Mice / Spastin: metabolism / Tubulin
                      (NLM Chemicals) / Peptide Synthases (NLM Chemicals) /
                      tubulin polyglutamylase (NLM Chemicals) / Polyglutamic Acid
                      (NLM Chemicals) / Spastin (NLM Chemicals)},
      cin          = {AG Misgeld},
      ddc          = {500},
      cid          = {I:(DE-2719)1110000-4},
      pnm          = {351 - Brain Function (POF4-351)},
      pid          = {G:(DE-HGF)POF4-351},
      typ          = {PUB:(DE-HGF)16},
      pubmed       = {pmid:40562742},
      pmc          = {pmc:PMC12198417},
      doi          = {10.1038/s41467-025-60855-6},
      url          = {https://pub.dzne.de/record/279358},
}