Home > Publications Database > Gain efficiency with streamlined and automated data processing: Examples from high-throughput monoclonal antibody production. > print

001     279428
005     20250710100949.0
024 7 _ |a 10.1371/journal.pone.0326678
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037 _ _ |a DZNE-2025-00759
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082 _ _ |a 610
100 1 _ |a Kotowicz, Malwina
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245 _ _ |a Gain efficiency with streamlined and automated data processing: Examples from high-throughput monoclonal antibody production.
260 _ _ |a San Francisco, California, US
|c 2025
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520 _ _ |a Data management and sample tracking in complex biological workflows are essential steps to ensure necessary documentation and guarantee reusability of data and metadata. Currently, these steps pose challenges related to correct annotation and labeling, error detection, and safeguarding the quality of documentation. With growing acquisition of biological data and the expanding automatization of laboratory workflows, manual processing of sample data is no longer favorable, as it is time- and resource-consuming, prone to biases and errors, and lacks scalability and standardization. Thus, managing heterogeneous biological data calls for efficient and tailored systems, especially in laboratories run by biologists with limited computational expertise. Here, we showcase how to meet these challenges with a modular pipeline for data processing, facilitating the complex production of monoclonal antibodies from single B-cells. We present best practices for development of data processing pipelines concerned with extensive acquisition of biological data that undergoes continuous manipulation and analysis. Moreover, we assess the versatility of proposed design principles through a proof-of-concept data processing pipeline for automated induced pluripotent stem cell culture and differentiation. We show that our approach streamlines data management operations, speeds up experimental cycles and leads to enhanced reproducibility. Finally, adhering to the presented guidelines will promote compliance with FAIR principles upon publishing.
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650 _ 7 |a Antibodies, Monoclonal
|2 NLM Chemicals
650 _ 2 |a Antibodies, Monoclonal: biosynthesis
|2 MeSH
650 _ 2 |a Humans
|2 MeSH
650 _ 2 |a Animals
|2 MeSH
650 _ 2 |a Induced Pluripotent Stem Cells: cytology
|2 MeSH
650 _ 2 |a Induced Pluripotent Stem Cells: metabolism
|2 MeSH
650 _ 2 |a B-Lymphocytes: immunology
|2 MeSH
650 _ 2 |a B-Lymphocytes: cytology
|2 MeSH
650 _ 2 |a B-Lymphocytes: metabolism
|2 MeSH
650 _ 2 |a Reproducibility of Results
|2 MeSH
650 _ 2 |a High-Throughput Screening Assays: methods
|2 MeSH
650 _ 2 |a Cell Differentiation
|2 MeSH
650 _ 2 |a Workflow
|2 MeSH
650 _ 2 |a Automation
|2 MeSH
700 1 _ |a Shumanska, Magdalena
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700 1 _ |a Fengler, Sven
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700 1 _ |a Kurkowsky, Birgit
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700 1 _ |a Meyer-Berhorn, Anja
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700 1 _ |a Moretti, Elisa
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700 1 _ |a Blersch, Josephine
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700 1 _ |a Schmidt, Gisela
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700 1 _ |a Kreye, Jakob
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700 1 _ |a van Hoof, Scott
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700 1 _ |a Sánchez-Sendín, Elisa
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700 1 _ |a Reincke, S Momsen
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700 1 _ |a Krüger, Lars
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700 1 _ |a Prüß, Harald
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700 1 _ |a Denner, Philip
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700 1 _ |a Fava, Eugenio
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700 1 _ |a Stappert, Dominik
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773 _ _ |a 10.1371/journal.pone.0326678
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