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@MISC{Mller:279496,
      author       = {Müller, Stephan A and Lichtenthaler, Stefan},
      title        = {{D}ataset: {P}roteomics of {ADAM}17 and i{R}hom2 {KO}
                      microglia using high-performance secretome protein
                      enrichment with click sugars},
      publisher    = {PRoteomics IDEntifications Database},
      reportid     = {DZNE-2025-00823},
      year         = {2025},
      abstract     = {The cell surface receptor TREM2 is a key genetic risk
                      factor and drug target in Alzheimer’s disease (AD). In the
                      brain, TREM2 is expressed in microglia, where it undergoes
                      proteolytic cleavage, linked to AD risk, but the responsible
                      protease in microglia is unknown. Another
                      microglia-expressed AD risk factor is inactive rhomboid 2
                      (iRhom2, RHBDF2), which acts as a non-catalytic subunit of
                      the metalloprotease ADAM17. Its function in AD is unknown.
                      To determine whether loss of iRhom2 and ADAM17 leads to a
                      reduction of cleavage of additional membrane proteins beyond
                      TNF, we used the ‘high-performance secretome protein
                      enrichment with click sugars’ (hiSPECS) method for mass
                      spectrometry-based secretome analysis (Tüshaus et al,
                      2020). hiSPECS uses a metabolic labeling with click sugars,
                      which allows to culture cells in the presence of serum or
                      serum-like supplements. Therefore, we have used the murine
                      microglia of wild-type and RHBDF2/iRhom2 KO mice.},
      cin          = {AG Lichtenthaler},
      cid          = {I:(DE-2719)1110006},
      pnm          = {352 - Disease Mechanisms (POF4-352)},
      pid          = {G:(DE-HGF)POF4-352},
      typ          = {PUB:(DE-HGF)32},
      url          = {https://pub.dzne.de/record/279496},
}