000279499 001__ 279499
000279499 005__ 20250709100939.0
000279499 037__ $$aDZNE-2025-00826
000279499 1001_ $$0P:(DE-2719)2810938$$aMüller, Stephan A$$b0$$udzne
000279499 245__ $$aDataset: Endogenous retroviruses promote prion-like spreading of proteopathic seeds
000279499 260__ $$bPRoteomics IDEntifications Database$$c2023
000279499 3367_ $$2BibTeX$$aMISC
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000279499 520__ $$aPrion-like spreading of protein misfolding is characteristic for neurodegenerative diseases, but the exact mechanisms of intercellular protein aggregate dissemination remain unresolved. Evidence accumulates that endogenous retroviruses, remnants of viral germline infections that are normally epigenetically silenced, become upregulated in neurodegenerative diseases such as amyotrophic lateral sclerosis and tauopathies. Here we uncover that activation of endogenous retroviruses affects prion-like spreading of proteopathic seeds. To identify changes in the proteome of donor cells that might contribute to protein aggregate spreading, we performed mass spectrometry analyses of total cell lysates and donor EV fractions using N2a cells expressing HA epitope-tagged Sup35 NM prion protein at early (P07) and late passages (P16) post cryopreservation. Among the proteins increased in donor cells and EVs upon prolonged culture, we identified mouse endogenous MLV retrovirus proteins to be highly increased.
000279499 536__ $$0G:(DE-HGF)POF4-352$$a352 - Disease Mechanisms (POF4-352)$$cPOF4-352$$fPOF IV$$x0
000279499 7001_ $$0P:(DE-2719)2181459$$aLichtenthaler, Stefan$$b1$$udzne
000279499 7870_ $$0DZNE-2023-00808$$aLiu, Shu et.al.$$d[London] : Nature Publishing Group UK, 2023$$iRelatedTo$$r$$tReactivated endogenous retroviruses promote protein aggregate spreading.
000279499 8564_ $$uhttps://wwwdev.ebi.ac.uk/pride/archive/projects/PXD043201
000279499 909CO $$ooai:pub.dzne.de:279499$$pVDB
000279499 9101_ $$0I:(DE-588)1065079516$$6P:(DE-2719)2810938$$aDeutsches Zentrum für Neurodegenerative Erkrankungen$$b0$$kDZNE
000279499 9101_ $$0I:(DE-588)1065079516$$6P:(DE-2719)2181459$$aDeutsches Zentrum für Neurodegenerative Erkrankungen$$b1$$kDZNE
000279499 9131_ $$0G:(DE-HGF)POF4-352$$1G:(DE-HGF)POF4-350$$2G:(DE-HGF)POF4-300$$3G:(DE-HGF)POF4$$4G:(DE-HGF)POF$$aDE-HGF$$bGesundheit$$lNeurodegenerative Diseases$$vDisease Mechanisms$$x0
000279499 9201_ $$0I:(DE-2719)1110006$$kAG Lichtenthaler$$lNeuroproteomics$$x0
000279499 980__ $$adataset
000279499 980__ $$aVDB
000279499 980__ $$aI:(DE-2719)1110006
000279499 980__ $$aUNRESTRICTED