TY  - JOUR
AU  - Bremer, Anna-Sophie
AU  - Henschel, Nico
AU  - Burkard, Hannah
AU  - Bernis, Maria Eugenia
AU  - Ulas, Thomas
AU  - Sabir, Hemmen
TI  - Transcriptomic profile of microglia following inflammation-sensitized hypoxic-ischemic brain injury in neonatal rats suggests strong contribution to neutrophil chemotaxis and activation.
JO  - Journal of neuroinflammation
VL  - 22
IS  - 1
SN  - 1742-2094
CY  - London
PB  - BioMed Central
M1  - DZNE-2025-00870
SP  - 189
PY  - 2025
AB  - Inflammation-sensitized hypoxic-ischemic brain injury significantly contributes to neonatal mortality as affected neonates do not benefit from standard cooling treatments. To get further insight into inflammatory responses involved, we experimentally investigated the immune response of microglia in an inflammation-sensitized neonatal hypoxia-ischemia (HI) model.Transcriptomic analysis of microglia isolated from brains following inflammation-sensitized HI brain injury revealed a strong upregulation of leukocyte recruitment and pro-inflammatory markers. Specifically, markers associated with neutrophil-mediated immune responses and chemotaxis were upregulated in the inflammation-sensitized HI group compared to the non-inflammation-sensitized HI and control groups. Serpine 1 and Selp could be identified as specifically upregulated markers indicating an acute inflammatory condition before HI injury.Our study revealed preliminary data about a microglia population which is primed to recruit peripheral neutrophils to infiltrate the brain and mediate neutrophil immune response. We showed a contribution to neutrophil activation in case of inflammation following HI in the brain. Targeting microglia-mediated neutrophil recruitment can indicate a possible treatment approach in case of inflammation-sensitized HI brain injury.
KW  - Animals
KW  - Microglia: metabolism
KW  - Microglia: pathology
KW  - Hypoxia-Ischemia, Brain: pathology
KW  - Hypoxia-Ischemia, Brain: metabolism
KW  - Hypoxia-Ischemia, Brain: immunology
KW  - Hypoxia-Ischemia, Brain: genetics
KW  - Animals, Newborn
KW  - Rats
KW  - Neutrophils: metabolism
KW  - Neutrophils: immunology
KW  - Transcriptome: physiology
KW  - Inflammation: pathology
KW  - Inflammation: metabolism
KW  - Neutrophil Activation: physiology
KW  - Chemotaxis: physiology
KW  - Gene Expression Profiling
KW  - Rats, Sprague-Dawley
KW  - Brain inflammation (Other)
KW  - Microglia priming (Other)
KW  - Neonatal Hypoxia-Ischemia (Other)
KW  - Neutrophil recruitment (Other)
KW  - Transcriptomic analysis (Other)
LB  - PUB:(DE-HGF)16
C6  - pmid:40684206
DO  - DOI:10.1186/s12974-025-03516-1
UR  - https://pub.dzne.de/record/280026
ER  -