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000280054 1001_ $$0P:(DE-2719)2812074$$aGemünd, Ioanna$$b0$$eFirst author$$udzne
000280054 245__ $$aCharacterization of human CMV-specific CD8+ T cells using multi-layer single-cell omics.
000280054 260__ $$aCambridge, MA$$bCell Press$$c2025
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000280054 520__ $$aIn this study, we established a comprehensive workflow to collect multi-omics single-cell data using a commercially available micro-well-based platform. This included whole transcriptome, cell surface markers (targeted sequencing-based cell surface proteomics), T cell specificities, adaptive immune receptor repertoire (AIRR) profiles, and sample multiplexing. With this technique, we identified paired T cell receptor sequences for three prominent human CMV epitopes. In addition, we assessed the ability of dCODE dextramers to detect antigen-specific T cells at low frequencies by estimating sensitivities and specificities when used as reagents for single-cell multi-omics.
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000280054 650_7 $$2Other$$aBD Rhapsody
000280054 650_7 $$2Other$$aCD8(+) T cells
000280054 650_7 $$2Other$$aCMV
000280054 650_7 $$2Other$$aCP: immunology
000280054 650_7 $$2Other$$aTCR sequencing
000280054 650_7 $$2Other$$aantigen-specific T cells
000280054 650_7 $$2Other$$adCODE dextramers
000280054 650_7 $$2Other$$asingle-cell multi-omics
000280054 650_7 $$2Other$$asystems immunology
000280054 650_7 $$2NLM Chemicals$$aReceptors, Antigen, T-Cell
000280054 650_2 $$2MeSH$$aHumans
000280054 650_2 $$2MeSH$$aCytomegalovirus: immunology
000280054 650_2 $$2MeSH$$aSingle-Cell Analysis: methods
000280054 650_2 $$2MeSH$$aCD8-Positive T-Lymphocytes: immunology
000280054 650_2 $$2MeSH$$aCD8-Positive T-Lymphocytes: metabolism
000280054 650_2 $$2MeSH$$aProteomics: methods
000280054 650_2 $$2MeSH$$aTranscriptome
000280054 650_2 $$2MeSH$$aReceptors, Antigen, T-Cell: metabolism
000280054 650_2 $$2MeSH$$aCytomegalovirus Infections: immunology
000280054 693__ $$0EXP:(DE-2719)PRECISE-20190321$$5EXP:(DE-2719)PRECISE-20190321$$ePlatform for Single Cell Genomics and Epigenomics at DZNE  University of Bonn$$x0
000280054 7001_ $$0P:(DE-2719)9001512$$aBonaguro, Lorenzo$$b1$$udzne
000280054 7001_ $$0P:(DE-2719)2812750$$aBecker, Matthias$$b2$$udzne
000280054 7001_ $$0P:(DE-2719)9001774$$aMüller, Sophie$$b3$$udzne
000280054 7001_ $$0P:(DE-HGF)0$$aJoos, Clemens$$b4
000280054 7001_ $$0P:(DE-2719)9000846$$aDe Domenico, Elena$$b5$$udzne
000280054 7001_ $$0P:(DE-2719)2812913$$aAschenbrenner, Anna C$$b6$$udzne
000280054 7001_ $$0P:(DE-2719)2811660$$aSchultze, Joachim L$$b7$$udzne
000280054 7001_ $$0P:(DE-HGF)0$$aMoosmann, Andreas$$b8
000280054 7001_ $$0P:(DE-2719)2812219$$aBeyer, Marc D$$b9$$eLast author$$udzne
000280054 773__ $$0PERI:(DE-600)3091714-1$$a10.1016/j.crmeth.2025.101085$$gVol. 5, no. 7, p. 101085 -$$n7$$p101085$$tCell reports / Methods$$v5$$x2667-2375$$y2025
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