Home > Publications Database > Characterization of human CMV-specific CD8+ T cells using multi-layer single-cell omics. > print

001     280054
005     20250824001734.0
024 7 _ |a 10.1016/j.crmeth.2025.101085
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037 _ _ |a DZNE-2025-00891
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100 1 _ |a Gemünd, Ioanna
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245 _ _ |a Characterization of human CMV-specific CD8+ T cells using multi-layer single-cell omics.
260 _ _ |a Cambridge, MA
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|b Cell Press
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520 _ _ |a In this study, we established a comprehensive workflow to collect multi-omics single-cell data using a commercially available micro-well-based platform. This included whole transcriptome, cell surface markers (targeted sequencing-based cell surface proteomics), T cell specificities, adaptive immune receptor repertoire (AIRR) profiles, and sample multiplexing. With this technique, we identified paired T cell receptor sequences for three prominent human CMV epitopes. In addition, we assessed the ability of dCODE dextramers to detect antigen-specific T cells at low frequencies by estimating sensitivities and specificities when used as reagents for single-cell multi-omics.
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650 _ 7 |a BD Rhapsody
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650 _ 7 |a CD8(+) T cells
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650 _ 7 |a CMV
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650 _ 7 |a CP: immunology
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650 _ 7 |a TCR sequencing
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650 _ 7 |a antigen-specific T cells
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650 _ 7 |a dCODE dextramers
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650 _ 7 |a single-cell multi-omics
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650 _ 7 |a systems immunology
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650 _ 7 |a Receptors, Antigen, T-Cell
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650 _ 2 |a Humans
|2 MeSH
650 _ 2 |a Cytomegalovirus: immunology
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650 _ 2 |a Single-Cell Analysis: methods
|2 MeSH
650 _ 2 |a CD8-Positive T-Lymphocytes: immunology
|2 MeSH
650 _ 2 |a CD8-Positive T-Lymphocytes: metabolism
|2 MeSH
650 _ 2 |a Proteomics: methods
|2 MeSH
650 _ 2 |a Transcriptome
|2 MeSH
650 _ 2 |a Receptors, Antigen, T-Cell: metabolism
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650 _ 2 |a Cytomegalovirus Infections: immunology
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700 1 _ |a Bonaguro, Lorenzo
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700 1 _ |a Becker, Matthias
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700 1 _ |a Müller, Sophie
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700 1 _ |a Joos, Clemens
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700 1 _ |a De Domenico, Elena
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700 1 _ |a Aschenbrenner, Anna C
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700 1 _ |a Schultze, Joachim L
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700 1 _ |a Moosmann, Andreas
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700 1 _ |a Beyer, Marc D
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773 _ _ |a 10.1016/j.crmeth.2025.101085
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