000280240 001__ 280240
000280240 005__ 20250831001814.0
000280240 0247_ $$2doi$$a10.1038/s41593-025-02002-4
000280240 0247_ $$2pmid$$apmid:40629141
000280240 0247_ $$2pmc$$apmc:PMC12321584
000280240 0247_ $$2ISSN$$a1097-6256
000280240 0247_ $$2ISSN$$a1546-1726
000280240 0247_ $$2altmetric$$aaltmetric:179085987
000280240 037__ $$aDZNE-2025-00918
000280240 041__ $$aEnglish
000280240 082__ $$a610
000280240 1001_ $$aOgunmowo, Tyler H$$b0
000280240 245__ $$aIntersectin and endophilin condensates prime synaptic vesicles for release site replenishment.
000280240 260__ $$aNew York, NY$$bNature America$$c2025
000280240 3367_ $$2DRIVER$$aarticle
000280240 3367_ $$2DataCite$$aOutput Types/Journal article
000280240 3367_ $$0PUB:(DE-HGF)16$$2PUB:(DE-HGF)$$aJournal Article$$bjournal$$mjournal$$s1756201305_31524
000280240 3367_ $$2BibTeX$$aARTICLE
000280240 3367_ $$2ORCID$$aJOURNAL_ARTICLE
000280240 3367_ $$00$$2EndNote$$aJournal Article
000280240 520__ $$aFollowing synaptic vesicle fusion, vacated release sites are replenished immediately by new vesicles for subsequent neurotransmission. These replacement vesicles are assumed to be located near release sites and used by chance. Here we find in mouse hippocampal excitatory synapses that replacement vesicles are clustered near the active zone where release sites reside by intersectin-1. Specifically, intersectin-1 forms dynamic molecular condensates with endophilin A1 and sequesters vesicles around this region. In the absence of intersectin-1, fewer vesicles cluster within 20 nm of the plasma membrane, and consequently vacated sites cannot be replenished rapidly, leading to synaptic depression. Mutations in intersectin-1 that disrupt endophilin A1 binding result in similar phenotypes. In the absence of endophilin A1, intersectin-1 is mislocalized, and this replacement pool of vesicles cannot be accessed, suggesting that endophilin A1 is needed to mobilize these vesicles. Thus, our work describes the replacement zone within a synapse, where replacement vesicles are stored for replenishment of the release site.
000280240 536__ $$0G:(DE-HGF)POF4-351$$a351 - Brain Function (POF4-351)$$cPOF4-351$$fPOF IV$$x0
000280240 588__ $$aDataset connected to CrossRef, PubMed, , Journals: pub.dzne.de
000280240 650_7 $$2NLM Chemicals$$aAdaptor Proteins, Vesicular Transport
000280240 650_7 $$2NLM Chemicals$$aintersectin 1
000280240 650_7 $$2NLM Chemicals$$aendophilin A1 protein, mouse
000280240 650_7 $$2NLM Chemicals$$aAdaptor Proteins, Signal Transducing
000280240 650_2 $$2MeSH$$aAnimals
000280240 650_2 $$2MeSH$$aSynaptic Vesicles: metabolism
000280240 650_2 $$2MeSH$$aSynaptic Vesicles: ultrastructure
000280240 650_2 $$2MeSH$$aSynaptic Vesicles: physiology
000280240 650_2 $$2MeSH$$aMice
000280240 650_2 $$2MeSH$$aHippocampus: cytology
000280240 650_2 $$2MeSH$$aHippocampus: metabolism
000280240 650_2 $$2MeSH$$aAdaptor Proteins, Vesicular Transport: metabolism
000280240 650_2 $$2MeSH$$aAdaptor Proteins, Vesicular Transport: genetics
000280240 650_2 $$2MeSH$$aSynapses: metabolism
000280240 650_2 $$2MeSH$$aSynapses: ultrastructure
000280240 650_2 $$2MeSH$$aNeurons
000280240 650_2 $$2MeSH$$aAdaptor Proteins, Signal Transducing: metabolism
000280240 650_2 $$2MeSH$$aAdaptor Proteins, Signal Transducing: genetics
000280240 650_2 $$2MeSH$$aSynaptic Transmission: physiology
000280240 650_2 $$2MeSH$$aMice, Inbred C57BL
000280240 650_2 $$2MeSH$$aMutation: genetics
000280240 7001_ $$0P:(DE-2719)9000582$$aHoffmann, Christian$$b1$$eFirst author$$udzne
000280240 7001_ $$00000-0001-6245-2406$$aPatel, Chintan$$b2
000280240 7001_ $$aPepper, Renee$$b3
000280240 7001_ $$0P:(DE-2719)9001572$$aWang, Han$$b4$$eFirst author
000280240 7001_ $$aGowrisankaran, Sindhuja$$b5
000280240 7001_ $$aIdel, Johanna$$b6
000280240 7001_ $$00009-0004-9724-9853$$aHo, Annie$$b7
000280240 7001_ $$aRaychaudhuri, Sumana$$b8
000280240 7001_ $$00000-0001-5930-885X$$aMaher, Brady J$$b9
000280240 7001_ $$00000-0003-2374-9922$$aCooper, Benjamin H$$b10
000280240 7001_ $$00000-0001-6440-3763$$aMilosevic, Ira$$b11
000280240 7001_ $$0P:(DE-2719)9000670$$aMilovanovic, Dragomir$$b12
000280240 7001_ $$00000-0001-7580-8141$$aWatanabe, Shigeki$$b13
000280240 773__ $$0PERI:(DE-600)1494955-6$$a10.1038/s41593-025-02002-4$$gVol. 28, no. 8, p. 1649 - 1662$$n8$$p1649 - 1662$$tNature neuroscience$$v28$$x1097-6256$$y2025
000280240 8564_ $$uhttps://pub.dzne.de/record/280240/files/DZNE-2025-00918%20SUP%2BSRC.zip
000280240 8564_ $$uhttps://pub.dzne.de/record/280240/files/DZNE-2025-00918.pdf$$yOpenAccess
000280240 8564_ $$uhttps://pub.dzne.de/record/280240/files/DZNE-2025-00918.pdf?subformat=pdfa$$xpdfa$$yOpenAccess
000280240 909CO $$ooai:pub.dzne.de:280240$$pdnbdelivery$$pdriver$$pVDB$$popen_access$$popenaire
000280240 9101_ $$0I:(DE-588)1065079516$$6P:(DE-2719)9000582$$aDeutsches Zentrum für Neurodegenerative Erkrankungen$$b1$$kDZNE
000280240 9101_ $$0I:(DE-588)1065079516$$6P:(DE-2719)9001572$$aDeutsches Zentrum für Neurodegenerative Erkrankungen$$b4$$kDZNE
000280240 9101_ $$0I:(DE-588)1065079516$$6P:(DE-2719)9000670$$aDeutsches Zentrum für Neurodegenerative Erkrankungen$$b12$$kDZNE
000280240 9131_ $$0G:(DE-HGF)POF4-351$$1G:(DE-HGF)POF4-350$$2G:(DE-HGF)POF4-300$$3G:(DE-HGF)POF4$$4G:(DE-HGF)POF$$aDE-HGF$$bGesundheit$$lNeurodegenerative Diseases$$vBrain Function$$x0
000280240 9141_ $$y2025
000280240 915__ $$0StatID:(DE-HGF)0200$$2StatID$$aDBCoverage$$bSCOPUS$$d2025-01-07
000280240 915__ $$0StatID:(DE-HGF)0160$$2StatID$$aDBCoverage$$bEssential Science Indicators$$d2025-01-07
000280240 915__ $$0StatID:(DE-HGF)1050$$2StatID$$aDBCoverage$$bBIOSIS Previews$$d2025-01-07
000280240 915__ $$0StatID:(DE-HGF)1190$$2StatID$$aDBCoverage$$bBiological Abstracts$$d2025-01-07
000280240 915__ $$0StatID:(DE-HGF)0600$$2StatID$$aDBCoverage$$bEbsco Academic Search$$d2025-01-07
000280240 915__ $$0StatID:(DE-HGF)0100$$2StatID$$aJCR$$bNAT NEUROSCI : 2022$$d2025-01-07
000280240 915__ $$0StatID:(DE-HGF)1030$$2StatID$$aDBCoverage$$bCurrent Contents - Life Sciences$$d2025-01-07
000280240 915__ $$0StatID:(DE-HGF)3003$$2StatID$$aDEAL Nature$$d2025-01-07$$wger
000280240 915__ $$0StatID:(DE-HGF)0113$$2StatID$$aWoS$$bScience Citation Index Expanded$$d2025-01-07
000280240 915__ $$0StatID:(DE-HGF)0150$$2StatID$$aDBCoverage$$bWeb of Science Core Collection$$d2025-01-07
000280240 915__ $$0StatID:(DE-HGF)0510$$2StatID$$aOpenAccess
000280240 915__ $$0StatID:(DE-HGF)0030$$2StatID$$aPeer Review$$bASC$$d2025-01-07
000280240 915__ $$0StatID:(DE-HGF)9925$$2StatID$$aIF >= 25$$bNAT NEUROSCI : 2022$$d2025-01-07
000280240 915__ $$0StatID:(DE-HGF)0300$$2StatID$$aDBCoverage$$bMedline$$d2025-01-07
000280240 915__ $$0LIC:(DE-HGF)CCBY4$$2HGFVOC$$aCreative Commons Attribution CC BY 4.0
000280240 915__ $$0StatID:(DE-HGF)0420$$2StatID$$aNationallizenz$$d2025-01-07$$wger
000280240 915__ $$0StatID:(DE-HGF)0199$$2StatID$$aDBCoverage$$bClarivate Analytics Master Journal List$$d2025-01-07
000280240 9201_ $$0I:(DE-2719)1813002$$kAG Milovanovic (Berlin)$$lMolecular Neuroscience$$x0
000280240 9201_ $$0I:(DE-2719)1013043$$kAG Milovanovic (Bonn)$$lMolecular Neuroscience$$x1
000280240 980__ $$ajournal
000280240 980__ $$aVDB
000280240 980__ $$aUNRESTRICTED
000280240 980__ $$aI:(DE-2719)1813002
000280240 980__ $$aI:(DE-2719)1013043
000280240 9801_ $$aFullTexts