Home > Publications Database > Intersectin and endophilin condensates prime synaptic vesicles for release site replenishment. > print

001     280240
005     20250831001814.0
024 7 _ |a 10.1038/s41593-025-02002-4
|2 doi
024 7 _ |a pmid:40629141
|2 pmid
024 7 _ |a pmc:PMC12321584
|2 pmc
024 7 _ |a 1097-6256
|2 ISSN
024 7 _ |a 1546-1726
|2 ISSN
024 7 _ |a altmetric:179085987
|2 altmetric
037 _ _ |a DZNE-2025-00918
041 _ _ |a English
082 _ _ |a 610
100 1 _ |a Ogunmowo, Tyler H
|b 0
245 _ _ |a Intersectin and endophilin condensates prime synaptic vesicles for release site replenishment.
260 _ _ |a New York, NY
|c 2025
|b Nature America
336 7 _ |a article
|2 DRIVER
336 7 _ |a Output Types/Journal article
|2 DataCite
336 7 _ |a Journal Article
|b journal
|m journal
|0 PUB:(DE-HGF)16
|s 1756201305_31524
|2 PUB:(DE-HGF)
336 7 _ |a ARTICLE
|2 BibTeX
336 7 _ |a JOURNAL_ARTICLE
|2 ORCID
336 7 _ |a Journal Article
|0 0
|2 EndNote
520 _ _ |a Following synaptic vesicle fusion, vacated release sites are replenished immediately by new vesicles for subsequent neurotransmission. These replacement vesicles are assumed to be located near release sites and used by chance. Here we find in mouse hippocampal excitatory synapses that replacement vesicles are clustered near the active zone where release sites reside by intersectin-1. Specifically, intersectin-1 forms dynamic molecular condensates with endophilin A1 and sequesters vesicles around this region. In the absence of intersectin-1, fewer vesicles cluster within 20 nm of the plasma membrane, and consequently vacated sites cannot be replenished rapidly, leading to synaptic depression. Mutations in intersectin-1 that disrupt endophilin A1 binding result in similar phenotypes. In the absence of endophilin A1, intersectin-1 is mislocalized, and this replacement pool of vesicles cannot be accessed, suggesting that endophilin A1 is needed to mobilize these vesicles. Thus, our work describes the replacement zone within a synapse, where replacement vesicles are stored for replenishment of the release site.
536 _ _ |a 351 - Brain Function (POF4-351)
|0 G:(DE-HGF)POF4-351
|c POF4-351
|f POF IV
|x 0
588 _ _ |a Dataset connected to CrossRef, PubMed, , Journals: pub.dzne.de
650 _ 7 |a Adaptor Proteins, Vesicular Transport
|2 NLM Chemicals
650 _ 7 |a intersectin 1
|2 NLM Chemicals
650 _ 7 |a endophilin A1 protein, mouse
|2 NLM Chemicals
650 _ 7 |a Adaptor Proteins, Signal Transducing
|2 NLM Chemicals
650 _ 2 |a Animals
|2 MeSH
650 _ 2 |a Synaptic Vesicles: metabolism
|2 MeSH
650 _ 2 |a Synaptic Vesicles: ultrastructure
|2 MeSH
650 _ 2 |a Synaptic Vesicles: physiology
|2 MeSH
650 _ 2 |a Mice
|2 MeSH
650 _ 2 |a Hippocampus: cytology
|2 MeSH
650 _ 2 |a Hippocampus: metabolism
|2 MeSH
650 _ 2 |a Adaptor Proteins, Vesicular Transport: metabolism
|2 MeSH
650 _ 2 |a Adaptor Proteins, Vesicular Transport: genetics
|2 MeSH
650 _ 2 |a Synapses: metabolism
|2 MeSH
650 _ 2 |a Synapses: ultrastructure
|2 MeSH
650 _ 2 |a Neurons
|2 MeSH
650 _ 2 |a Adaptor Proteins, Signal Transducing: metabolism
|2 MeSH
650 _ 2 |a Adaptor Proteins, Signal Transducing: genetics
|2 MeSH
650 _ 2 |a Synaptic Transmission: physiology
|2 MeSH
650 _ 2 |a Mice, Inbred C57BL
|2 MeSH
650 _ 2 |a Mutation: genetics
|2 MeSH
700 1 _ |a Hoffmann, Christian
|0 P:(DE-2719)9000582
|b 1
|e First author
|u dzne
700 1 _ |a Patel, Chintan
|0 0000-0001-6245-2406
|b 2
700 1 _ |a Pepper, Renee
|b 3
700 1 _ |a Wang, Han
|0 P:(DE-2719)9001572
|b 4
|e First author
700 1 _ |a Gowrisankaran, Sindhuja
|b 5
700 1 _ |a Idel, Johanna
|b 6
700 1 _ |a Ho, Annie
|0 0009-0004-9724-9853
|b 7
700 1 _ |a Raychaudhuri, Sumana
|b 8
700 1 _ |a Maher, Brady J
|0 0000-0001-5930-885X
|b 9
700 1 _ |a Cooper, Benjamin H
|0 0000-0003-2374-9922
|b 10
700 1 _ |a Milosevic, Ira
|0 0000-0001-6440-3763
|b 11
700 1 _ |a Milovanovic, Dragomir
|0 P:(DE-2719)9000670
|b 12
700 1 _ |a Watanabe, Shigeki
|0 0000-0001-7580-8141
|b 13
773 _ _ |a 10.1038/s41593-025-02002-4
|g Vol. 28, no. 8, p. 1649 - 1662
|0 PERI:(DE-600)1494955-6
|n 8
|p 1649 - 1662
|t Nature neuroscience
|v 28
|y 2025
|x 1097-6256
856 4 _ |u https://pub.dzne.de/record/280240/files/DZNE-2025-00918%20SUP%2BSRC.zip
856 4 _ |y OpenAccess
|u https://pub.dzne.de/record/280240/files/DZNE-2025-00918.pdf
856 4 _ |y OpenAccess
|x pdfa
|u https://pub.dzne.de/record/280240/files/DZNE-2025-00918.pdf?subformat=pdfa
909 C O |o oai:pub.dzne.de:280240
|p openaire
|p open_access
|p VDB
|p driver
|p dnbdelivery
910 1 _ |a Deutsches Zentrum für Neurodegenerative Erkrankungen
|0 I:(DE-588)1065079516
|k DZNE
|b 1
|6 P:(DE-2719)9000582
910 1 _ |a Deutsches Zentrum für Neurodegenerative Erkrankungen
|0 I:(DE-588)1065079516
|k DZNE
|b 4
|6 P:(DE-2719)9001572
910 1 _ |a Deutsches Zentrum für Neurodegenerative Erkrankungen
|0 I:(DE-588)1065079516
|k DZNE
|b 12
|6 P:(DE-2719)9000670
913 1 _ |a DE-HGF
|b Gesundheit
|l Neurodegenerative Diseases
|1 G:(DE-HGF)POF4-350
|0 G:(DE-HGF)POF4-351
|3 G:(DE-HGF)POF4
|2 G:(DE-HGF)POF4-300
|4 G:(DE-HGF)POF
|v Brain Function
|x 0
914 1 _ |y 2025
915 _ _ |a DBCoverage
|0 StatID:(DE-HGF)0200
|2 StatID
|b SCOPUS
|d 2025-01-07
915 _ _ |a DBCoverage
|0 StatID:(DE-HGF)0160
|2 StatID
|b Essential Science Indicators
|d 2025-01-07
915 _ _ |a DBCoverage
|0 StatID:(DE-HGF)1050
|2 StatID
|b BIOSIS Previews
|d 2025-01-07
915 _ _ |a DBCoverage
|0 StatID:(DE-HGF)1190
|2 StatID
|b Biological Abstracts
|d 2025-01-07
915 _ _ |a DBCoverage
|0 StatID:(DE-HGF)0600
|2 StatID
|b Ebsco Academic Search
|d 2025-01-07
915 _ _ |a JCR
|0 StatID:(DE-HGF)0100
|2 StatID
|b NAT NEUROSCI : 2022
|d 2025-01-07
915 _ _ |a DBCoverage
|0 StatID:(DE-HGF)1030
|2 StatID
|b Current Contents - Life Sciences
|d 2025-01-07
915 _ _ |a DEAL Nature
|0 StatID:(DE-HGF)3003
|2 StatID
|d 2025-01-07
|w ger
915 _ _ |a WoS
|0 StatID:(DE-HGF)0113
|2 StatID
|b Science Citation Index Expanded
|d 2025-01-07
915 _ _ |a DBCoverage
|0 StatID:(DE-HGF)0150
|2 StatID
|b Web of Science Core Collection
|d 2025-01-07
915 _ _ |a OpenAccess
|0 StatID:(DE-HGF)0510
|2 StatID
915 _ _ |a Peer Review
|0 StatID:(DE-HGF)0030
|2 StatID
|b ASC
|d 2025-01-07
915 _ _ |a IF >= 25
|0 StatID:(DE-HGF)9925
|2 StatID
|b NAT NEUROSCI : 2022
|d 2025-01-07
915 _ _ |a DBCoverage
|0 StatID:(DE-HGF)0300
|2 StatID
|b Medline
|d 2025-01-07
915 _ _ |a Creative Commons Attribution CC BY 4.0
|0 LIC:(DE-HGF)CCBY4
|2 HGFVOC
915 _ _ |a Nationallizenz
|0 StatID:(DE-HGF)0420
|2 StatID
|d 2025-01-07
|w ger
915 _ _ |a DBCoverage
|0 StatID:(DE-HGF)0199
|2 StatID
|b Clarivate Analytics Master Journal List
|d 2025-01-07
920 1 _ |0 I:(DE-2719)1813002
|k AG Milovanovic (Berlin)
|l Molecular Neuroscience
|x 0
920 1 _ |0 I:(DE-2719)1013043
|k AG Milovanovic (Bonn)
|l Molecular Neuroscience
|x 1
980 _ _ |a journal
980 _ _ |a VDB
980 _ _ |a UNRESTRICTED
980 _ _ |a I:(DE-2719)1813002
980 _ _ |a I:(DE-2719)1013043
980 1 _ |a FullTexts

LibraryCollectionCLSMajorCLSMinorLanguageAuthor
Marc 21