000280243 001__ 280243
000280243 005__ 20250827101637.0
000280243 020__ $$a978-1-0716-4681-6 (print)
000280243 020__ $$a978-1-0716-4682-3 (electronic)
000280243 0247_ $$2doi$$a10.1007/7651_2024_574
000280243 0247_ $$2pmid$$apmid:39570548
000280243 0247_ $$2ISSN$$a1064-3745
000280243 0247_ $$2ISSN$$a1940-6029
000280243 037__ $$aDZNE-2025-00921
000280243 041__ $$aEnglish
000280243 082__ $$a570
000280243 1001_ $$0P:(DE-2719)9000564$$aGrass, Tobias$$b0$$eFirst author$$udzne
000280243 245__ $$aNeuromuscular Organoids to Study Spinal Cord Development and Disease.
000280243 260__ $$aNew York, NY$$bSpringer US$$c2025
000280243 29510 $$aOrganoids / Turksen, Kursad (Editor) ; New York, NY : Springer US, 2025, Chapter 574 ; ISSN: 1064-3745=1940-6029 ; ISBN: 978-1-0716-4681-6=978-1-0716-4682-3 ; doi:10.1007/978-1-0716-4682-3
000280243 300__ $$a197 - 219
000280243 3367_ $$2ORCID$$aBOOK_CHAPTER
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000280243 3367_ $$2DRIVER$$abookPart
000280243 3367_ $$2BibTeX$$aINBOOK
000280243 3367_ $$2DataCite$$aOutput Types/Book chapter
000280243 3367_ $$0PUB:(DE-HGF)7$$2PUB:(DE-HGF)$$aContribution to a book$$bcontb$$mcontb$$s1756201732_31524
000280243 4900_ $$aMethods in Molecular Biology$$v2951
000280243 520__ $$aMany aspects of neurodegenerative disease pathology remain unresolved. Why do certain neuronal subpopulations acquire vulnerability to stress or mutations in ubiquitously expressed genes, while others remain resilient? Do these neurons harbor intrinsic marks that make them prone to degeneration? Do these diseases have a neurodevelopmental component? Lacking this fundamental knowledge hampers the discovery of efficacious treatments. While it is well established that human organoids enable the modeling of brain-related diseases, we still lack an organoid model that recapitulates the regionalization complexity and physiology of the spinal cord. Here, we describe an advanced experimental protocol to generate neuromuscular organoids composed of a wide rostro-caudal (RC) diversity of spinal motor neurons (spMNs) and mesodermal progenitor-derived muscle cells. This model therefore allows for the robust and reproducible study of neuromuscular unit development and disease.
000280243 536__ $$0G:(DE-HGF)POF4-352$$a352 - Disease Mechanisms (POF4-352)$$cPOF4-352$$fPOF IV$$x0
000280243 588__ $$aDataset connected to CrossRef Book Series, PubMed, , Journals: pub.dzne.de
000280243 650_7 $$2Other$$aHuman induced pluripotent stem cells
000280243 650_7 $$2Other$$aNeuromesodermal progenitors
000280243 650_7 $$2Other$$aNeuromuscular spinal cord organoids
000280243 650_7 $$2Other$$aSkeletal and smooth muscle
000280243 650_7 $$2Other$$aSpinal motor neurons
000280243 650_2 $$2MeSH$$aOrganoids: cytology
000280243 650_2 $$2MeSH$$aOrganoids: metabolism
000280243 650_2 $$2MeSH$$aSpinal Cord: cytology
000280243 650_2 $$2MeSH$$aSpinal Cord: growth & development
000280243 650_2 $$2MeSH$$aSpinal Cord: pathology
000280243 650_2 $$2MeSH$$aHumans
000280243 650_2 $$2MeSH$$aMotor Neurons: cytology
000280243 650_2 $$2MeSH$$aMotor Neurons: metabolism
000280243 650_2 $$2MeSH$$aAnimals
000280243 650_2 $$2MeSH$$aMice
000280243 650_2 $$2MeSH$$aNeuromuscular Junction
000280243 7001_ $$0P:(DE-2719)9002364$$aDokuzluoglu, Zeynep$$b1$$udzne
000280243 7001_ $$0P:(DE-2719)9000726$$aRodríguez-Muela, Natalia$$b2$$eLast author$$udzne
000280243 773__ $$a10.1007/7651_2024_574
000280243 8564_ $$uhttps://pub.dzne.de/record/280243/files/DZNE-2025-00921_Restricted.pdf
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000280243 9101_ $$0I:(DE-588)1065079516$$6P:(DE-2719)9000564$$aDeutsches Zentrum für Neurodegenerative Erkrankungen$$b0$$kDZNE
000280243 9101_ $$0I:(DE-588)1065079516$$6P:(DE-2719)9002364$$aDeutsches Zentrum für Neurodegenerative Erkrankungen$$b1$$kDZNE
000280243 9101_ $$0I:(DE-588)1065079516$$6P:(DE-2719)9000726$$aDeutsches Zentrum für Neurodegenerative Erkrankungen$$b2$$kDZNE
000280243 9131_ $$0G:(DE-HGF)POF4-352$$1G:(DE-HGF)POF4-350$$2G:(DE-HGF)POF4-300$$3G:(DE-HGF)POF4$$4G:(DE-HGF)POF$$aDE-HGF$$bGesundheit$$lNeurodegenerative Diseases$$vDisease Mechanisms$$x0
000280243 9141_ $$y2025
000280243 915__ $$0StatID:(DE-HGF)0200$$2StatID$$aDBCoverage$$bSCOPUS$$d2024-12-19
000280243 915__ $$0StatID:(DE-HGF)0300$$2StatID$$aDBCoverage$$bMedline$$d2024-12-19
000280243 9201_ $$0I:(DE-2719)1713001$$kAG Rodriguez-Muela$$lSelective Neuronal Vulnerability in Neurodegenerative Diseases$$x0
000280243 980__ $$acontb
000280243 980__ $$aVDB
000280243 980__ $$aI:(DE-2719)1713001
000280243 980__ $$aUNRESTRICTED