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@MISC{Tutas:280736,
author = {Tutas, Janine and Kononenko, Natalia L. and Tutas, Janine
and Tolve, Marianna},
othercontributors = {Özer-Yildiz, Ebru and Ickert, Lotte and Klein, Ines and
Silverman, Quinn and Liebsch, Filip and Dethloff, Frederik
and Giavalisco, Patrick and Endepols, Heike and
Georgomanolis, Theodoros and Neumaier, Bernd and Drzezga,
Alexander and Schwarz, Günter and Thorens, Bernard and
Gatto, Graziana and Frezza, Christian and Kononenko, Natalia
L.},
title = {{D}ataset: {A}utophagy regulator {ATG}5 maintains
cerebellar function by preventing its excessive glycolytic
activity.},
publisher = {Zenodo},
reportid = {DZNE-2025-00957},
year = {2024},
abstract = {The uploaded metabolomic dataset contains
liquid-chromatography-mass spectrometry (LC-MS) data
associated to the publication of Janine Tutas et al. from
the Natalia Kononenko laboratory. The title of the
publication is: Autophagy regulator ATG5 preserves
cerebellar function by safeguarding its glycolytic activity.
This article is to be published in Nature Metabolism Article
Abstract Dysfunctions in autophagy, a cellular mechanism for
breaking down components within lysosomes, often lead to
neurodegeneration. The specific mechanisms underlying
neuronal vulnerability to autophagy dysfunction remain
elusive. Here, we show that autophagy contributes to the
survival of cerebellar Purkinje cells (PCs) by safeguarding
their glycolytic activity. This function of autophagy is
independent of its conventional housekeeping role and
involves ATG5-mediated regulation of glucose transporter 2
(GLUT2) levels during cerebellar maturation.
Autophagy-deficient PCs exhibit GLUT2 accumulation on the
plasma membrane, along with increased glucose uptake and
alterations in glycolysis. We identify lysophosphatidic acid
and serine as glycolytic intermediates that trigger PC death
and demonstrate that deletion of GLUT2 in ATG5-deficient
mice mitigates PC neurodegeneration and restores their
ataxic gait. Taken together, this work reveals a novel
mechanism for regulating GLUT2 levels in neurons and
provides new insights into the neuroprotective role of
autophagy by controlling glucose homeostasis in the brain.},
keywords = {ATG5 (Other) / autophagy (Other) / glycolysis (Other) /
Purkinje cells (Other) / neurodegeneration (Other)},
cin = {AG Boecker},
cid = {I:(DE-2719)1011202},
pnm = {353 - Clinical and Health Care Research (POF4-353)},
pid = {G:(DE-HGF)POF4-353},
typ = {PUB:(DE-HGF)32},
doi = {10.5281/zenodo.10635079},
url = {https://pub.dzne.de/record/280736},
}