Home > Publications Database > Disrupted Myelination in FAHN: Insights from a Patient-Specific hiPSC Neuron-Oligodendrocyte Model. > print

001     280907
005     20250905102149.0
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037 _ _ |a DZNE-2025-00991
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100 1 _ |a Efendic, Fatima
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245 _ _ |a Disrupted Myelination in FAHN: Insights from a Patient-Specific hiPSC Neuron-Oligodendrocyte Model.
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520 _ _ |a Fatty-acid-hydroxylase-associated neurodegeneration (FAHN) is a rare neurodegenerative disorder caused by loss-of-function mutations in the FA2H gene, leading to impaired enzymatic activity and resulting in myelin sheath instability, demyelination, and axonal degeneration. In this study, we established a human in vitro model using neurons and oligodendrocytes derived from induced pluripotent stem cells (hiPSCs) of a FAHN patient. This coculture system enabled the investigation of myelination processes and myelin integrity in a disease-relevant context. Analyses using immunofluorescence and Western blot revealed impaired expression and localisation of key myelin proteins in oligodendrocytes and cocultures. FA2H-deficient cells showed reduced myelination, shortened internodes, and disrupted formation of the nodes of Ranvier. Additionally, we identified autophagy defects-a hallmark of many neurodegenerative diseases-including reduced p62 expression, elevated LC3B levels, and impaired fusion of autophagosomes with lysosomes. This study presents a robust hiPSC-based model to study FAHN, offering new insights into the molecular pathology of the disease. Our findings suggest that FA2H mutations compromise both the structural integrity of myelin and the efficiency of the autophagic machinery, highlighting potential targets for future therapeutic interventions.
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650 _ 7 |a FA2H
|2 Other
650 _ 7 |a FAHN
|2 Other
650 _ 7 |a autophagy
|2 Other
650 _ 7 |a demyelination
|2 Other
650 _ 7 |a induced pluripotent stem cells
|2 Other
650 _ 7 |a myelin proteins
|2 Other
650 _ 7 |a neurons
|2 Other
650 _ 7 |a oligodendrocytes
|2 Other
650 _ 7 |a Mixed Function Oxygenases
|0 EC 1.-
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650 _ 2 |a Humans
|2 MeSH
650 _ 2 |a Induced Pluripotent Stem Cells: metabolism
|2 MeSH
650 _ 2 |a Induced Pluripotent Stem Cells: pathology
|2 MeSH
650 _ 2 |a Myelin Sheath: metabolism
|2 MeSH
650 _ 2 |a Myelin Sheath: pathology
|2 MeSH
650 _ 2 |a Neurons: metabolism
|2 MeSH
650 _ 2 |a Neurons: pathology
|2 MeSH
650 _ 2 |a Oligodendroglia: metabolism
|2 MeSH
650 _ 2 |a Oligodendroglia: pathology
|2 MeSH
650 _ 2 |a Autophagy
|2 MeSH
650 _ 2 |a Models, Biological
|2 MeSH
650 _ 2 |a Neurodegenerative Diseases: pathology
|2 MeSH
650 _ 2 |a Neurodegenerative Diseases: genetics
|2 MeSH
650 _ 2 |a Neurodegenerative Diseases: metabolism
|2 MeSH
650 _ 2 |a Mutation: genetics
|2 MeSH
650 _ 2 |a Mixed Function Oxygenases: genetics
|2 MeSH
650 _ 2 |a Mixed Function Oxygenases: metabolism
|2 MeSH
650 _ 2 |a Coculture Techniques
|2 MeSH
700 1 _ |a Hermann, Andreas
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700 1 _ |a Frech, Moritz J
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773 _ _ |a 10.3390/cells14161261
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910 1 _ |a Deutsches Zentrum für Neurodegenerative Erkrankungen
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