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@ARTICLE{Scifo:280964,
author = {Scifo, Enzo and Morsy, Sarah and Liu, Ting and Xie, Kan and
Schaaf, Kristina and Bano, Daniele and Ehninger, Dan},
title = {{P}roteomic aging signatures across mouse organs and life
stages.},
journal = {The EMBO journal},
volume = {44},
number = {16},
issn = {0261-4189},
address = {[London]},
publisher = {Nature Publishing Group UK},
reportid = {DZNE-2025-01046},
pages = {4631 - 4660},
year = {2025},
abstract = {Aging is associated with the accumulation of molecular
damage, functional decline, increasing disease prevalence,
and ultimately mortality. Although our system-wide
understanding of aging has significantly progressed at the
genomic and transcriptomic levels, the availability of
large-scale proteomic datasets remains limited. To address
this gap, we have conducted an unbiased quantitative
proteomic analysis in male C57BL/6J mice, examining eight
key organs (brain, heart, lung, liver, kidney, spleen,
skeletal muscle, and testis) across six life stages (3, 5,
8, 14, 20, and 26-month-old animals). Our results reveal
age-associated organ-specific as well as systemic proteomic
alterations, with the earliest and most extensive changes
observed in the kidney and spleen, followed by liver and
lung, while the proteomic profiles of brain, heart, testis,
and skeletal muscle remain more stable. Isolation of the
non-blood-associated proteome allowed us to identify
organ-specific aging processes, including oxidative
phosphorylation in the kidney and lipid metabolism in the
liver, alongside shared aging signatures. Trajectory and
network analyses further reveal key protein hubs linked to
age-related proteomic shifts. These results provide a
system-level resource of protein changes during aging in
mice, and identify potential molecular regulators of
age-related decline.},
keywords = {Aging (Other) / Mass Spectrometry (Other) / Mouse Organs
(Other) / Protein Trajectories (Other) / SureQuant (Other)},
cin = {AG Ehninger / AG Bano},
ddc = {570},
cid = {I:(DE-2719)1013005 / I:(DE-2719)1013003},
pnm = {352 - Disease Mechanisms (POF4-352) / 351 - Brain Function
(POF4-351)},
pid = {G:(DE-HGF)POF4-352 / G:(DE-HGF)POF4-351},
typ = {PUB:(DE-HGF)16},
pubmed = {pmid:40664891},
pmc = {pmc:PMC12361549},
doi = {10.1038/s44318-025-00509-x},
url = {https://pub.dzne.de/record/280964},
}