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000281358 1001_ $$0P:(DE-2719)9002015$$aChhabra, Akshita$$b0$$eFirst author$$udzne
000281358 245__ $$aCondensates of synaptic vesicles and synapsin-1 mediate actin sequestering and polymerization.
000281358 260__ $$a[London]$$bNature Publishing Group UK$$c2025
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000281358 520__ $$aNeuronal communication relies on precisely maintained synaptic vesicle (SV) clusters, which assemble via liquid-liquid phase separation. This process requires synapsins, the major synaptic phosphoproteins, which are known to bind actin. Reorganization of SVs, synapsins, and actin is a hallmark of synaptic activity, but the molecular details of the interactions between these components remain unclear. Here, we combine in vitro reconstitution with expansion microscopy, super-resolution imaging, and cryo-electron tomography to dissect the roles of SV-synapsin-1 condensates in the organization of the presynaptic actin cytoskeleton. Our results indicate that condensation of synapsin-1 initiates actin polymerization. This process enables SV-synapsin-actin assemblies to facilitate the mesoscale organization of SV clusters along axons, which is similar to the native presynaptic organization observed at both lamprey and mammalian synapses. Understanding the relationship between the actin network and synapsin-synaptic vesicle condensates can help elucidate how coordinated neurotransmission along the axon enables circuit function and behavior.
000281358 536__ $$0G:(DE-HGF)POF4-351$$a351 - Brain Function (POF4-351)$$cPOF4-351$$fPOF IV$$x0
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000281358 650_7 $$2Other$$aActin
000281358 650_7 $$2Other$$aLiquid-Liquid Phase Separation
000281358 650_7 $$2Other$$aPresynapses
000281358 650_7 $$2Other$$aSynapsin
000281358 650_7 $$2Other$$aSynaptic Vesicles
000281358 650_7 $$2NLM Chemicals$$aSynapsins
000281358 650_7 $$2NLM Chemicals$$aActins
000281358 650_2 $$2MeSH$$aSynaptic Vesicles: metabolism
000281358 650_2 $$2MeSH$$aSynaptic Vesicles: ultrastructure
000281358 650_2 $$2MeSH$$aSynapsins: metabolism
000281358 650_2 $$2MeSH$$aAnimals
000281358 650_2 $$2MeSH$$aActins: metabolism
000281358 650_2 $$2MeSH$$aPolymerization
000281358 650_2 $$2MeSH$$aActin Cytoskeleton: metabolism
000281358 650_2 $$2MeSH$$aSynapses: metabolism
000281358 7001_ $$0P:(DE-2719)9000582$$aHoffmann, Christian$$b1$$eFirst author
000281358 7001_ $$0P:(DE-2719)9002012$$aAguilar Perez, Gerard$$b2$$eFirst author$$udzne
000281358 7001_ $$0P:(DE-2719)9002676$$aKorobeinikov, Aleksandr A$$b3
000281358 7001_ $$00000-0002-5728-7255$$aRentsch, Jakob$$b4
000281358 7001_ $$00000-0002-4349-4886$$aHümpfer, Nadja$$b5
000281358 7001_ $$0P:(DE-2719)9001437$$aKokwaro, Linda$$b6
000281358 7001_ $$0P:(DE-2719)9002532$$aGnidovec, Luka$$b7$$udzne
000281358 7001_ $$00000-0003-4448-4230$$aPetrović, Arsen$$b8
000281358 7001_ $$00000-0001-5150-9390$$aWallace, Jaqulin N$$b9
000281358 7001_ $$0P:(DE-2719)9002092$$aTromm, Johannes Vincent$$b10
000281358 7001_ $$00000-0003-1613-9130$$aRomán-Vendrell, Cristina$$b11
000281358 7001_ $$00009-0005-6288-0546$$aJohnson, Emma C$$b12
000281358 7001_ $$0P:(DE-2719)9001749$$aRanković, Branislava$$b13
000281358 7001_ $$aPerego, Eleonora$$b14
000281358 7001_ $$00000-0002-5451-6710$$aVolpi, Tommaso$$b15
000281358 7001_ $$00000-0002-8366-7622$$aFernández-Busnadiego, Rubén$$b16
000281358 7001_ $$00000-0002-0009-1024$$aKöster, Sarah$$b17
000281358 7001_ $$00000-0002-1667-7839$$aRizzoli, Silvio O$$b18
000281358 7001_ $$00000-0003-3948-4332$$aEwers, Helge$$b19
000281358 7001_ $$00000-0001-9915-6360$$aMorgan, Jennifer R$$b20
000281358 7001_ $$0P:(DE-2719)9000670$$aMilovanovic, Dragomir$$b21$$eLast author$$udzne
000281358 773__ $$0PERI:(DE-600)1467419-1$$a10.1038/s44318-025-00516-y$$gVol. 44, no. 18, p. 5112 - 5148$$n18$$p5112 - 5148$$tThe EMBO journal$$v44$$x0261-4189$$y2025
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