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001     281358
005     20251112102900.0
024 7 _ |a 10.1038/s44318-025-00516-y
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024 7 _ |a 1460-2075
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037 _ _ |a DZNE-2025-01105
041 _ _ |a English
082 _ _ |a 570
100 1 _ |a Chhabra, Akshita
|0 P:(DE-2719)9002015
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245 _ _ |a Condensates of synaptic vesicles and synapsin-1 mediate actin sequestering and polymerization.
260 _ _ |a [London]
|c 2025
|b Nature Publishing Group UK
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520 _ _ |a Neuronal communication relies on precisely maintained synaptic vesicle (SV) clusters, which assemble via liquid-liquid phase separation. This process requires synapsins, the major synaptic phosphoproteins, which are known to bind actin. Reorganization of SVs, synapsins, and actin is a hallmark of synaptic activity, but the molecular details of the interactions between these components remain unclear. Here, we combine in vitro reconstitution with expansion microscopy, super-resolution imaging, and cryo-electron tomography to dissect the roles of SV-synapsin-1 condensates in the organization of the presynaptic actin cytoskeleton. Our results indicate that condensation of synapsin-1 initiates actin polymerization. This process enables SV-synapsin-actin assemblies to facilitate the mesoscale organization of SV clusters along axons, which is similar to the native presynaptic organization observed at both lamprey and mammalian synapses. Understanding the relationship between the actin network and synapsin-synaptic vesicle condensates can help elucidate how coordinated neurotransmission along the axon enables circuit function and behavior.
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650 _ 7 |a Actin
|2 Other
650 _ 7 |a Liquid-Liquid Phase Separation
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650 _ 7 |a Presynapses
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650 _ 7 |a Synapsin
|2 Other
650 _ 7 |a Synaptic Vesicles
|2 Other
650 _ 7 |a Synapsins
|2 NLM Chemicals
650 _ 7 |a Actins
|2 NLM Chemicals
650 _ 2 |a Synaptic Vesicles: metabolism
|2 MeSH
650 _ 2 |a Synaptic Vesicles: ultrastructure
|2 MeSH
650 _ 2 |a Synapsins: metabolism
|2 MeSH
650 _ 2 |a Animals
|2 MeSH
650 _ 2 |a Actins: metabolism
|2 MeSH
650 _ 2 |a Polymerization
|2 MeSH
650 _ 2 |a Actin Cytoskeleton: metabolism
|2 MeSH
650 _ 2 |a Synapses: metabolism
|2 MeSH
700 1 _ |a Hoffmann, Christian
|0 P:(DE-2719)9000582
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700 1 _ |a Aguilar Perez, Gerard
|0 P:(DE-2719)9002012
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700 1 _ |a Korobeinikov, Aleksandr A
|0 P:(DE-2719)9002676
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700 1 _ |a Rentsch, Jakob
|0 0000-0002-5728-7255
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700 1 _ |a Hümpfer, Nadja
|0 0000-0002-4349-4886
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700 1 _ |a Kokwaro, Linda
|0 P:(DE-2719)9001437
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700 1 _ |a Gnidovec, Luka
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700 1 _ |a Petrović, Arsen
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700 1 _ |a Wallace, Jaqulin N
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700 1 _ |a Tromm, Johannes Vincent
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700 1 _ |a Román-Vendrell, Cristina
|0 0000-0003-1613-9130
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700 1 _ |a Johnson, Emma C
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700 1 _ |a Ranković, Branislava
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700 1 _ |a Perego, Eleonora
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700 1 _ |a Volpi, Tommaso
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700 1 _ |a Fernández-Busnadiego, Rubén
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700 1 _ |a Köster, Sarah
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700 1 _ |a Rizzoli, Silvio O
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700 1 _ |a Ewers, Helge
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700 1 _ |a Morgan, Jennifer R
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700 1 _ |a Milovanovic, Dragomir
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773 _ _ |a 10.1038/s44318-025-00516-y
|g Vol. 44, no. 18, p. 5112 - 5148
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