Home > Publications Database > Condensates of synaptic vesicles and synapsin-1 mediate actin sequestering and polymerization. > print

001     281358
005     20251012002037.0
024 7 _ |a 10.1038/s44318-025-00516-y
|2 doi
024 7 _ |a pmid:40813925
|2 pmid
024 7 _ |a pmc:PMC12436662
|2 pmc
024 7 _ |a 0261-4189
|2 ISSN
024 7 _ |a 1460-2075
|2 ISSN
024 7 _ |a altmetric:180316241
|2 altmetric
037 _ _ |a DZNE-2025-01105
041 _ _ |a English
082 _ _ |a 570
100 1 _ |a Chhabra, Akshita
|0 P:(DE-2719)9002015
|b 0
|e First author
|u dzne
245 _ _ |a Condensates of synaptic vesicles and synapsin-1 mediate actin sequestering and polymerization.
260 _ _ |a [London]
|c 2025
|b Nature Publishing Group UK
336 7 _ |a article
|2 DRIVER
336 7 _ |a Output Types/Journal article
|2 DataCite
336 7 _ |a Journal Article
|b journal
|m journal
|0 PUB:(DE-HGF)16
|s 1759826604_17321
|2 PUB:(DE-HGF)
336 7 _ |a ARTICLE
|2 BibTeX
336 7 _ |a JOURNAL_ARTICLE
|2 ORCID
336 7 _ |a Journal Article
|0 0
|2 EndNote
520 _ _ |a Neuronal communication relies on precisely maintained synaptic vesicle (SV) clusters, which assemble via liquid-liquid phase separation. This process requires synapsins, the major synaptic phosphoproteins, which are known to bind actin. Reorganization of SVs, synapsins, and actin is a hallmark of synaptic activity, but the molecular details of the interactions between these components remain unclear. Here, we combine in vitro reconstitution with expansion microscopy, super-resolution imaging, and cryo-electron tomography to dissect the roles of SV-synapsin-1 condensates in the organization of the presynaptic actin cytoskeleton. Our results indicate that condensation of synapsin-1 initiates actin polymerization. This process enables SV-synapsin-actin assemblies to facilitate the mesoscale organization of SV clusters along axons, which is similar to the native presynaptic organization observed at both lamprey and mammalian synapses. Understanding the relationship between the actin network and synapsin-synaptic vesicle condensates can help elucidate how coordinated neurotransmission along the axon enables circuit function and behavior.
536 _ _ |a 351 - Brain Function (POF4-351)
|0 G:(DE-HGF)POF4-351
|c POF4-351
|f POF IV
|x 0
588 _ _ |a Dataset connected to CrossRef, PubMed, , Journals: pub.dzne.de
650 _ 7 |a Actin
|2 Other
650 _ 7 |a Liquid-Liquid Phase Separation
|2 Other
650 _ 7 |a Presynapses
|2 Other
650 _ 7 |a Synapsin
|2 Other
650 _ 7 |a Synaptic Vesicles
|2 Other
650 _ 7 |a Synapsins
|2 NLM Chemicals
650 _ 7 |a Actins
|2 NLM Chemicals
650 _ 2 |a Synaptic Vesicles: metabolism
|2 MeSH
650 _ 2 |a Synaptic Vesicles: ultrastructure
|2 MeSH
650 _ 2 |a Synapsins: metabolism
|2 MeSH
650 _ 2 |a Animals
|2 MeSH
650 _ 2 |a Actins: metabolism
|2 MeSH
650 _ 2 |a Polymerization
|2 MeSH
650 _ 2 |a Actin Cytoskeleton: metabolism
|2 MeSH
650 _ 2 |a Synapses: metabolism
|2 MeSH
700 1 _ |a Hoffmann, Christian
|0 P:(DE-2719)9000582
|b 1
|e First author
700 1 _ |a Aguilar Perez, Gerard
|0 P:(DE-2719)9002012
|b 2
|e First author
|u dzne
700 1 _ |a Korobeinikov, Aleksandr A
|0 P:(DE-2719)9002676
|b 3
700 1 _ |a Rentsch, Jakob
|0 0000-0002-5728-7255
|b 4
700 1 _ |a Hümpfer, Nadja
|0 0000-0002-4349-4886
|b 5
700 1 _ |a Kokwaro, Linda
|0 P:(DE-2719)9001437
|b 6
700 1 _ |a Gnidovec, Luka
|0 P:(DE-2719)9002532
|b 7
|u dzne
700 1 _ |a Petrović, Arsen
|0 0000-0003-4448-4230
|b 8
700 1 _ |a Wallace, Jaqulin N
|0 0000-0001-5150-9390
|b 9
700 1 _ |a Tromm, Johannes Vincent
|0 P:(DE-2719)9002092
|b 10
700 1 _ |a Román-Vendrell, Cristina
|0 0000-0003-1613-9130
|b 11
700 1 _ |a Johnson, Emma C
|0 0009-0005-6288-0546
|b 12
700 1 _ |a Ranković, Branislava
|0 P:(DE-2719)9001749
|b 13
700 1 _ |a Perego, Eleonora
|b 14
700 1 _ |a Volpi, Tommaso
|0 0000-0002-5451-6710
|b 15
700 1 _ |a Fernández-Busnadiego, Rubén
|0 0000-0002-8366-7622
|b 16
700 1 _ |a Köster, Sarah
|0 0000-0002-0009-1024
|b 17
700 1 _ |a Rizzoli, Silvio O
|0 0000-0002-1667-7839
|b 18
700 1 _ |a Ewers, Helge
|0 0000-0003-3948-4332
|b 19
700 1 _ |a Morgan, Jennifer R
|0 0000-0001-9915-6360
|b 20
700 1 _ |a Milovanovic, Dragomir
|0 P:(DE-2719)9000670
|b 21
|e Last author
|u dzne
773 _ _ |a 10.1038/s44318-025-00516-y
|g Vol. 44, no. 18, p. 5112 - 5148
|0 PERI:(DE-600)1467419-1
|n 18
|p 5112 - 5148
|t The EMBO journal
|v 44
|y 2025
|x 0261-4189
856 4 _ |u https://pub.dzne.de/record/281358/files/DZNE-2025-01105%20SUP.zip
856 4 _ |u https://pub.dzne.de/record/281358/files/DZNE-2025-01105.pdf
|y Restricted
856 4 _ |u https://pub.dzne.de/record/281358/files/DZNE-2025-01105.pdf?subformat=pdfa
|x pdfa
|y Restricted
909 C O |o oai:pub.dzne.de:281358
|p VDB
910 1 _ |a Deutsches Zentrum für Neurodegenerative Erkrankungen
|0 I:(DE-588)1065079516
|k DZNE
|b 0
|6 P:(DE-2719)9002015
910 1 _ |a Deutsches Zentrum für Neurodegenerative Erkrankungen
|0 I:(DE-588)1065079516
|k DZNE
|b 1
|6 P:(DE-2719)9000582
910 1 _ |a Deutsches Zentrum für Neurodegenerative Erkrankungen
|0 I:(DE-588)1065079516
|k DZNE
|b 2
|6 P:(DE-2719)9002012
910 1 _ |a Deutsches Zentrum für Neurodegenerative Erkrankungen
|0 I:(DE-588)1065079516
|k DZNE
|b 3
|6 P:(DE-2719)9002676
910 1 _ |a Deutsches Zentrum für Neurodegenerative Erkrankungen
|0 I:(DE-588)1065079516
|k DZNE
|b 6
|6 P:(DE-2719)9001437
910 1 _ |a Deutsches Zentrum für Neurodegenerative Erkrankungen
|0 I:(DE-588)1065079516
|k DZNE
|b 7
|6 P:(DE-2719)9002532
910 1 _ |a Deutsches Zentrum für Neurodegenerative Erkrankungen
|0 I:(DE-588)1065079516
|k DZNE
|b 10
|6 P:(DE-2719)9002092
910 1 _ |a Deutsches Zentrum für Neurodegenerative Erkrankungen
|0 I:(DE-588)1065079516
|k DZNE
|b 13
|6 P:(DE-2719)9001749
910 1 _ |a Deutsches Zentrum für Neurodegenerative Erkrankungen
|0 I:(DE-588)1065079516
|k DZNE
|b 21
|6 P:(DE-2719)9000670
913 1 _ |a DE-HGF
|b Gesundheit
|l Neurodegenerative Diseases
|1 G:(DE-HGF)POF4-350
|0 G:(DE-HGF)POF4-351
|3 G:(DE-HGF)POF4
|2 G:(DE-HGF)POF4-300
|4 G:(DE-HGF)POF
|v Brain Function
|x 0
914 1 _ |y 2025
915 _ _ |a DEAL Wiley
|0 StatID:(DE-HGF)3001
|2 StatID
|d 2024-12-30
|w ger
915 _ _ |a DBCoverage
|0 StatID:(DE-HGF)0200
|2 StatID
|b SCOPUS
|d 2024-12-30
915 _ _ |a DBCoverage
|0 StatID:(DE-HGF)0300
|2 StatID
|b Medline
|d 2024-12-30
915 _ _ |a DBCoverage
|0 StatID:(DE-HGF)0501
|2 StatID
|b DOAJ Seal
|d 2024-04-08T07:44:01Z
915 _ _ |a DBCoverage
|0 StatID:(DE-HGF)0500
|2 StatID
|b DOAJ
|d 2024-04-08T07:44:01Z
915 _ _ |a Peer Review
|0 StatID:(DE-HGF)0030
|2 StatID
|b DOAJ : Anonymous peer review, Double anonymous peer review
|d 2024-04-08T07:44:01Z
915 _ _ |a Creative Commons Attribution CC BY (No Version)
|0 LIC:(DE-HGF)CCBYNV
|2 V:(DE-HGF)
|b DOAJ
|d 2024-04-08T07:44:01Z
915 _ _ |a DBCoverage
|0 StatID:(DE-HGF)0199
|2 StatID
|b Clarivate Analytics Master Journal List
|d 2024-12-30
915 _ _ |a DBCoverage
|0 StatID:(DE-HGF)1050
|2 StatID
|b BIOSIS Previews
|d 2024-12-30
915 _ _ |a DBCoverage
|0 StatID:(DE-HGF)0160
|2 StatID
|b Essential Science Indicators
|d 2024-12-30
915 _ _ |a DBCoverage
|0 StatID:(DE-HGF)1030
|2 StatID
|b Current Contents - Life Sciences
|d 2024-12-30
915 _ _ |a DBCoverage
|0 StatID:(DE-HGF)1190
|2 StatID
|b Biological Abstracts
|d 2024-12-30
915 _ _ |a WoS
|0 StatID:(DE-HGF)0113
|2 StatID
|b Science Citation Index Expanded
|d 2024-12-30
915 _ _ |a DBCoverage
|0 StatID:(DE-HGF)0150
|2 StatID
|b Web of Science Core Collection
|d 2024-12-30
915 _ _ |a JCR
|0 StatID:(DE-HGF)0100
|2 StatID
|b EMBO J : 2022
|d 2024-12-30
915 _ _ |a DBCoverage
|0 StatID:(DE-HGF)0600
|2 StatID
|b Ebsco Academic Search
|d 2024-12-30
915 _ _ |a Peer Review
|0 StatID:(DE-HGF)0030
|2 StatID
|b ASC
|d 2024-12-30
915 _ _ |a IF >= 10
|0 StatID:(DE-HGF)9910
|2 StatID
|b EMBO J : 2022
|d 2024-12-30
915 _ _ |a Article Processing Charges
|0 StatID:(DE-HGF)0561
|2 StatID
|d 2024-12-30
915 _ _ |a Fees
|0 StatID:(DE-HGF)0700
|2 StatID
|d 2024-12-30
920 1 _ |0 I:(DE-2719)1813002
|k AG Milovanovic (Berlin)
|l Molecular Neuroscience
|x 0
980 _ _ |a journal
980 _ _ |a VDB
980 _ _ |a I:(DE-2719)1813002
980 _ _ |a UNRESTRICTED

LibraryCollectionCLSMajorCLSMinorLanguageAuthor
Marc 21