TY  - JOUR
AU  - Lorenzo-Diaz, Fabian
AU  - Klassert, Tilman E
AU  - Zubiria-Barrera, Cristina
AU  - Keles, Amelya
AU  - Gonzalez-Carracedo, Mario
AU  - Hernandez, Mariano
AU  - Slevogt, Hortense
AU  - Grünewald, Thomas
TI  - Clostridioides difficile evolution in a tertiary German hospital through a retrospective genomic characterization.
JO  - Infection
VL  - 53
IS  - 5
SN  - 0300-8126
CY  - München
PB  - Urban & Vogel
M1  - DZNE-2025-01122
SP  - 2209 - 2218
PY  - 2025
AB  - Clostridioides difficile is a major cause of healthcare-associated infections, contributing to significant morbidity and mortality. This study aimed to investigate the genomic characteristics, antimicrobial resistance (AMR) profiles, and temporal dynamics of C. difficile strains isolated from hospitalized patients in a German tertiary hospital over nearly two decades (1997-2015).Whole-genome sequencing was performed on 46 toxigenic C. difficile isolates to determine sequence types (STs) and phylogenetic relationships and these were compared to national surveillance data on C. dificile. AMR profiling was conducted to identify key resistance determinants at genetic level while epsilometer minimum inhibitory concentration (MIC) analyses were used to correlate genetic resistance markers with phenotypic resistance. Longitudinal antibiotic usage data were analysed to assess potential associations with resistance profiles and strains evolution.Five predominant STs were identified: ST1 (30
KW  - Clostridioides difficile: genetics
KW  - Clostridioides difficile: drug effects
KW  - Clostridioides difficile: classification
KW  - Clostridioides difficile: isolation & purification
KW  - Tertiary Care Centers
KW  - Humans
KW  - Germany: epidemiology
KW  - Clostridium Infections: microbiology
KW  - Clostridium Infections: epidemiology
KW  - Anti-Bacterial Agents: pharmacology
KW  - Retrospective Studies
KW  - Microbial Sensitivity Tests
KW  - Phylogeny
KW  - Whole Genome Sequencing
KW  - Cross Infection: microbiology
KW  - Cross Infection: epidemiology
KW  - Drug Resistance, Bacterial: genetics
KW  - Evolution, Molecular
KW  - Genome, Bacterial
KW  - Male
KW  - Female
KW  - Antibiotic resistance genes (Other)
KW  - CDI (Other)
KW  - Clostridioides difficile (Other)
KW  - PaLoc (Other)
KW  - WGS (Other)
KW  - Anti-Bacterial Agents (NLM Chemicals)
LB  - PUB:(DE-HGF)16
C6  - pmid:40483626
DO  - DOI:10.1007/s15010-025-02576-y
UR  - https://pub.dzne.de/record/281437
ER  -