%0 Journal Article
%A Li, Lucie Y
%A Höltje, Markus
%A Rasmussen, Helle Foverskov
%A Halle, Lennard
%A Mayrhofer, Marie
%A Blüthner, Martin
%A Prüss, Harald
%T Binding of established antinuclear antibodies to neurons depends on tissue fixation and underlying autoantigens.
%J Frontiers in immunology
%V 16
%@ 1664-3224
%C Lausanne
%I Frontiers Media
%M DZNE-2025-01206
%P 1674907
%D 2025
%X Antinuclear antibodies (ANAs) are central biomarkers in rheumatological conditions and can drive disease pathology. Much less is known about the role of ANAs in neurological symptoms, although a number of experimental studies have demonstrated direct effects on neuronal function, for example in neuropsychiatric lupus erythematosus. Moreover, it is unclear whether the ANAs detected in HEp-2 cell-based assays, the gold standard for ANA diagnostics, can also be recognized in modern screening assays for anti-neuronal autoimmunity, such as staining on rodent brain sections or neuronal cultures. In this study, we therefore conducted a comparative mapping of ANA-positive sera with well-characterized HEp-2 patterns to central nervous system (CNS) tissue, utilizing fixed and unfixed murine brain sections and primary murine neurons. We screened 74 ANA-positive sera classified into 14 individual patterns and combinations thereof. Majority of the samples reacted with fixed primary neurons (99
%K Animals
%K Neurons: immunology
%K Neurons: metabolism
%K Antibodies, Antinuclear: immunology
%K Antibodies, Antinuclear: metabolism
%K Mice
%K Autoantigens: immunology
%K Humans
%K Tissue Fixation
%K Brain: immunology
%K Brain: metabolism
%K Female
%K Male
%K ANA (Other)
%K HEp-2 (Other)
%K anti-neuronal antibodies (Other)
%K autoimmunity (Other)
%K immunofluorescence (Other)
%K Antibodies, Antinuclear (NLM Chemicals)
%K Autoantigens (NLM Chemicals)
%F PUB:(DE-HGF)16
%9 Journal Article
%$ pmid:41142797
%2 pmc:PMC12549672
%R 10.3389/fimmu.2025.1674907
%U https://pub.dzne.de/record/281825