TY  - JOUR
AU  - Li, Lucie Y
AU  - Höltje, Markus
AU  - Rasmussen, Helle Foverskov
AU  - Halle, Lennard
AU  - Mayrhofer, Marie
AU  - Blüthner, Martin
AU  - Prüss, Harald
TI  - Binding of established antinuclear antibodies to neurons depends on tissue fixation and underlying autoantigens.
JO  - Frontiers in immunology
VL  - 16
SN  - 1664-3224
CY  - Lausanne
PB  - Frontiers Media
M1  - DZNE-2025-01206
SP  - 1674907
PY  - 2025
AB  - Antinuclear antibodies (ANAs) are central biomarkers in rheumatological conditions and can drive disease pathology. Much less is known about the role of ANAs in neurological symptoms, although a number of experimental studies have demonstrated direct effects on neuronal function, for example in neuropsychiatric lupus erythematosus. Moreover, it is unclear whether the ANAs detected in HEp-2 cell-based assays, the gold standard for ANA diagnostics, can also be recognized in modern screening assays for anti-neuronal autoimmunity, such as staining on rodent brain sections or neuronal cultures. In this study, we therefore conducted a comparative mapping of ANA-positive sera with well-characterized HEp-2 patterns to central nervous system (CNS) tissue, utilizing fixed and unfixed murine brain sections and primary murine neurons. We screened 74 ANA-positive sera classified into 14 individual patterns and combinations thereof. Majority of the samples reacted with fixed primary neurons (99
KW  - Animals
KW  - Neurons: immunology
KW  - Neurons: metabolism
KW  - Antibodies, Antinuclear: immunology
KW  - Antibodies, Antinuclear: metabolism
KW  - Mice
KW  - Autoantigens: immunology
KW  - Humans
KW  - Tissue Fixation
KW  - Brain: immunology
KW  - Brain: metabolism
KW  - Female
KW  - Male
KW  - ANA (Other)
KW  - HEp-2 (Other)
KW  - anti-neuronal antibodies (Other)
KW  - autoimmunity (Other)
KW  - immunofluorescence (Other)
KW  - Antibodies, Antinuclear (NLM Chemicals)
KW  - Autoantigens (NLM Chemicals)
LB  - PUB:(DE-HGF)16
C6  - pmid:41142797
C2  - pmc:PMC12549672
DO  - DOI:10.3389/fimmu.2025.1674907
UR  - https://pub.dzne.de/record/281825
ER  -