TY  - JOUR
AU  - Mimoso, Tiago
AU  - Korobeinikov, Aleksandr
AU  - Stein, Alexander
AU  - Milovanovic, Dragomir
AU  - Rizzoli, Silvio O
AU  - Köster, Sarah
AU  - Reshetniak, Sofiia
TI  - Brain-derived synaptic vesicles have an intrinsic ability to sequester tubulin.
JO  - BMC biology
VL  - 23
IS  - 1
SN  - 1741-7007
CY  - Heidelberg
PB  - Springer
M1  - DZNE-2025-01264
SP  - 340
PY  - 2025
AB  - The presence and function of microtubules within the synaptic bouton has long been under investigation. In recent years, evidence has accumulated that connects the synaptic vesicle cluster to the local dynamics of microtubule ends. Nonetheless, one question remains open, namely whether the vesicles influence the availability of tubulin within the synaptic compartment.An analysis of previously published live imaging experiments indicates that tubulin is strongly enriched in the synaptic vesicle cluster. To analyze the vesicle-tubulin interaction directly, we isolated vesicles from the mouse brain and imaged them together with fluorescent tubulin in vitro. We found that soluble tubulin is collected by synaptic vesicles in physiological buffers, resulting in the formation of tubulin-rich regions (TRRs) on the respective vesicle clusters.We conclude that the synaptic vesicle cluster is indeed able to recruit soluble tubulin.
KW  - Animals
KW  - Tubulin: metabolism
KW  - Synaptic Vesicles: metabolism
KW  - Mice
KW  - Brain: metabolism
KW  - Microtubules: metabolism
KW  - Cytoskeleton (Other)
KW  - Microtubules (Other)
KW  - Synapse (Other)
KW  - Synaptic vesicle cluster (Other)
KW  - Synaptic vesicles (Other)
KW  - Tubulin (Other)
KW  - Tubulin (NLM Chemicals)
LB  - PUB:(DE-HGF)16
C6  - pmid:41239319
C2  - pmc:PMC12619239
DO  - DOI:10.1186/s12915-025-02464-9
UR  - https://pub.dzne.de/record/282294
ER  -