TY  - JOUR
AU  - Marcos Morgado, Barbara
AU  - Klafki, Hans-Wolfgang
AU  - Bauer, Chris
AU  - Waniek, Katharina
AU  - Esselmann, Hermann
AU  - Wirths, Oliver
AU  - Hansen, Niels
AU  - Lachmann, Ingolf
AU  - Osterloh, Dirk
AU  - Schuchhardt, Johannes
AU  - Wiltfang, Jens
TI  - Assessment of immunoprecipitation with subsequent immunoassays for the blood-based diagnosis of Alzheimer's disease.
JO  - Archiv für Psychiatrie und Nervenkrankheiten
VL  - Advance online publication
SN  - 1433-8491
CY  - Heidelberg
PB  - Springer
M1  - DZNE-2025-01274
SP  - -
PY  - 2024
N1  - ISSN 1433-8491 not unique: **2 hits**.
AB  - The Aβ42/40 ratio and the concentration of phosphorylated Tau181 in blood plasma represent attractive biomarkers for Alzheimer's disease. As a means for reducing potential matrix effects, which may interfere with plasma immunoassays, we have previously developed a pre-analytical sample workup by semi-automated immunoprecipitation. Here we test the compatibility of pre-analytical immunoprecipitations with automated Aβ1-40, Aβ1-42 and phosphorylated Tau181 immunoassays on the Lumipulse platform and compare the diagnostic performance of the respective immunoprecipitation immunoassay approaches with direct plasma measurements. 71 participants were dichotomized according to their Aβ42/40 ratios in cerebrospinal fluid into the diagnostic groups amyloid-positive (n = 32) and amyloid-negative (n = 39). The plasma Aβ1-42/1-40 ratio and phosphorylated Tau181 levels were determined on the Lumipulse G600II platform (Fujirebio) by direct measurements in EDTA-plasma or after Aβ- or Tau-immunoprecipitation, respectively. Pre-analytical immunoprecipitation of Aβ turned out to be compatible with the Lumipulse Aβ assays and resulted in a numerical, yet statistically not significant increase in the area under the ROC curve for plasma Aβ1-42/1-40. Additionally, we observed a significant increase in the standardised effect size (Cohen's D). Pre-analytical immunoprecipitation of Tau resulted in increased differences between the diagnostic groups in terms of median and mean phosphorylated Tau 181 levels. Furthermore, we observed a greater Cohen's d (p < 0.001) and a larger area under the ROC curve (p = 0.038) after Tau-IP. Our preliminary findings in a small, preselected sample indicate that pre-analytical immunoprecipitation may have the potential to improve the diagnostic performance of plasma biomarker immunoassays for Aβ1-42/1-40 and phosphorylated Tau181 to predict brain amyloid deposition.
KW  - Alzheimer’s disease (Other)
KW  - Amyloid beta (Other)
KW  - Biomarker (Other)
KW  - Blood plasma (Other)
KW  - Tau (Other)
LB  - PUB:(DE-HGF)16
C6  - pmid:38316685
DO  - DOI:10.1007/s00406-023-01751-2
UR  - https://pub.dzne.de/record/282304
ER  -