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@ARTICLE{MarcosMorgado:282304,
      author       = {Marcos Morgado, Barbara and Klafki, Hans-Wolfgang and
                      Bauer, Chris and Waniek, Katharina and Esselmann, Hermann
                      and Wirths, Oliver and Hansen, Niels and Lachmann, Ingolf
                      and Osterloh, Dirk and Schuchhardt, Johannes and Wiltfang,
                      Jens},
      title        = {{A}ssessment of immunoprecipitation with subsequent
                      immunoassays for the blood-based diagnosis of {A}lzheimer's
                      disease.},
      journal      = {Archiv für Psychiatrie und Nervenkrankheiten},
      volume       = {Advance online publication},
      issn         = {1433-8491},
      address      = {Heidelberg},
      publisher    = {Springer},
      reportid     = {DZNE-2025-01274},
      pages        = {-},
      year         = {2024},
      note         = {ISSN 1433-8491 not unique: **2 hits**.},
      abstract     = {The Aβ42/40 ratio and the concentration of phosphorylated
                      Tau181 in blood plasma represent attractive biomarkers for
                      Alzheimer's disease. As a means for reducing potential
                      matrix effects, which may interfere with plasma
                      immunoassays, we have previously developed a pre-analytical
                      sample workup by semi-automated immunoprecipitation. Here we
                      test the compatibility of pre-analytical
                      immunoprecipitations with automated Aβ1-40, Aβ1-42 and
                      phosphorylated Tau181 immunoassays on the Lumipulse platform
                      and compare the diagnostic performance of the respective
                      immunoprecipitation immunoassay approaches with direct
                      plasma measurements. 71 participants were dichotomized
                      according to their Aβ42/40 ratios in cerebrospinal fluid
                      into the diagnostic groups amyloid-positive (n = 32) and
                      amyloid-negative (n = 39). The plasma Aβ1-42/1-40 ratio and
                      phosphorylated Tau181 levels were determined on the
                      Lumipulse G600II platform (Fujirebio) by direct measurements
                      in EDTA-plasma or after Aβ- or Tau-immunoprecipitation,
                      respectively. Pre-analytical immunoprecipitation of Aβ
                      turned out to be compatible with the Lumipulse Aβ assays
                      and resulted in a numerical, yet statistically not
                      significant increase in the area under the ROC curve for
                      plasma Aβ1-42/1-40. Additionally, we observed a significant
                      increase in the standardised effect size (Cohen's D).
                      Pre-analytical immunoprecipitation of Tau resulted in
                      increased differences between the diagnostic groups in terms
                      of median and mean phosphorylated Tau 181 levels.
                      Furthermore, we observed a greater Cohen's d (p < 0.001) and
                      a larger area under the ROC curve (p = 0.038) after Tau-IP.
                      Our preliminary findings in a small, preselected sample
                      indicate that pre-analytical immunoprecipitation may have
                      the potential to improve the diagnostic performance of
                      plasma biomarker immunoassays for Aβ1-42/1-40 and
                      phosphorylated Tau181 to predict brain amyloid deposition.},
      keywords     = {Alzheimer’s disease (Other) / Amyloid beta (Other) /
                      Biomarker (Other) / Blood plasma (Other) / Tau (Other)},
      cin          = {AG Wiltfang},
      ddc          = {610},
      cid          = {I:(DE-2719)1410006},
      pnm          = {353 - Clinical and Health Care Research (POF4-353)},
      pid          = {G:(DE-HGF)POF4-353},
      typ          = {PUB:(DE-HGF)16},
      pubmed       = {pmid:38316685},
      doi          = {10.1007/s00406-023-01751-2},
      url          = {https://pub.dzne.de/record/282304},
}